Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye
2016
Authors
Šekuljica, NatašaPrlainović, Nevena
Jovanović, Jelena
Stefanović, Andrea
Grbavčić, Sanja
Mijin, Dušan
Knežević-Jugović, Zorica
Article (Published version)
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Show full item recordAbstract
The problem of environmental pollution becomes more worrisome day by day, primarily due to the large amounts of wastewater contaminated with various harmful organic compounds, discharged untreated or partially clean into the environment. Feasibility of use of horseradish peroxidase (Amoracia rusticana) in the synthetic dyes decolorization was approved by many researchers. Among a number of supports used for the immobilization, it was found that natural clay, kaolin, has excellent features which are a precondition for obtaining biocatalysts with the excellent performances. For this reason, a horseradish peroxidase was immobilized onto kaolin using glutaraldehyde as a cross-linking agent. Obtained biocatalyst was applied in the decolorization of anthraquinone dye C.I. acid violet 109. Under determined optimal conditions (pH 4.0, hydrogen peroxide concentration 0.6 mM, dye concentration 30 mg L-1, temperature 24 degrees C) around 76% of dye decolorization was achieved. Reusability study s...howed that resulting biocatalyst was possible to apply four times in the desired reaction with relatively high decolorization percentage.
Keywords:
horseradish peroxidase / immobilization / wastewater / dyesSource:
Hemijska industrija, 2016, 70, 2, 217-224Publisher:
- Savez hemijskih inženjera, Beograd
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-MESTD-Basic Research (BR or ON)-172013)
DOI: 10.2298/HEMIND150220028S
ISSN: 0367-598X
WoS: 000376274600012
Scopus: 2-s2.0-84966658477
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Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Šekuljica, Nataša AU - Prlainović, Nevena AU - Jovanović, Jelena AU - Stefanović, Andrea AU - Grbavčić, Sanja AU - Mijin, Dušan AU - Knežević-Jugović, Zorica PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3251 AB - The problem of environmental pollution becomes more worrisome day by day, primarily due to the large amounts of wastewater contaminated with various harmful organic compounds, discharged untreated or partially clean into the environment. Feasibility of use of horseradish peroxidase (Amoracia rusticana) in the synthetic dyes decolorization was approved by many researchers. Among a number of supports used for the immobilization, it was found that natural clay, kaolin, has excellent features which are a precondition for obtaining biocatalysts with the excellent performances. For this reason, a horseradish peroxidase was immobilized onto kaolin using glutaraldehyde as a cross-linking agent. Obtained biocatalyst was applied in the decolorization of anthraquinone dye C.I. acid violet 109. Under determined optimal conditions (pH 4.0, hydrogen peroxide concentration 0.6 mM, dye concentration 30 mg L-1, temperature 24 degrees C) around 76% of dye decolorization was achieved. Reusability study showed that resulting biocatalyst was possible to apply four times in the desired reaction with relatively high decolorization percentage. PB - Savez hemijskih inženjera, Beograd T2 - Hemijska industrija T1 - Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye EP - 224 IS - 2 SP - 217 VL - 70 DO - 10.2298/HEMIND150220028S ER -
@article{ author = "Šekuljica, Nataša and Prlainović, Nevena and Jovanović, Jelena and Stefanović, Andrea and Grbavčić, Sanja and Mijin, Dušan and Knežević-Jugović, Zorica", year = "2016", abstract = "The problem of environmental pollution becomes more worrisome day by day, primarily due to the large amounts of wastewater contaminated with various harmful organic compounds, discharged untreated or partially clean into the environment. Feasibility of use of horseradish peroxidase (Amoracia rusticana) in the synthetic dyes decolorization was approved by many researchers. Among a number of supports used for the immobilization, it was found that natural clay, kaolin, has excellent features which are a precondition for obtaining biocatalysts with the excellent performances. For this reason, a horseradish peroxidase was immobilized onto kaolin using glutaraldehyde as a cross-linking agent. Obtained biocatalyst was applied in the decolorization of anthraquinone dye C.I. acid violet 109. Under determined optimal conditions (pH 4.0, hydrogen peroxide concentration 0.6 mM, dye concentration 30 mg L-1, temperature 24 degrees C) around 76% of dye decolorization was achieved. Reusability study showed that resulting biocatalyst was possible to apply four times in the desired reaction with relatively high decolorization percentage.", publisher = "Savez hemijskih inženjera, Beograd", journal = "Hemijska industrija", title = "Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye", pages = "224-217", number = "2", volume = "70", doi = "10.2298/HEMIND150220028S" }
Šekuljica, N., Prlainović, N., Jovanović, J., Stefanović, A., Grbavčić, S., Mijin, D.,& Knežević-Jugović, Z.. (2016). Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye. in Hemijska industrija Savez hemijskih inženjera, Beograd., 70(2), 217-224. https://doi.org/10.2298/HEMIND150220028S
Šekuljica N, Prlainović N, Jovanović J, Stefanović A, Grbavčić S, Mijin D, Knežević-Jugović Z. Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye. in Hemijska industrija. 2016;70(2):217-224. doi:10.2298/HEMIND150220028S .
Šekuljica, Nataša, Prlainović, Nevena, Jovanović, Jelena, Stefanović, Andrea, Grbavčić, Sanja, Mijin, Dušan, Knežević-Jugović, Zorica, "Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye" in Hemijska industrija, 70, no. 2 (2016):217-224, https://doi.org/10.2298/HEMIND150220028S . .