Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry
Само за регистроване кориснике
2016
Аутори
Simović, MilicaBezbradica, Dejan
Banjanac, Katarina
Milivojević, Ana
Fanuel, Mathieu
Rogniaux, Helene
Ropartz, David
Veličković, Dušan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Galacto-oligosaccharides (GOS) represent a diverse group of well-characterized prebiotic ingredients derived from lactose in a reaction catalyzed with beta-galactosidases. Enzymatic transgalactosylation results in a mixture of compounds of various degrees of polymerization and types of linkages. Because structure plays an important role in terms of prebiotic activity, it is of crucial importance to provide an insight into the mechanism of transgalactosylation reaction and occurrence of different types of beta-linkages during GOS synthesis. Our study proved that a novel one-step method, based on ion-mobility spectrometry tandem mass spectrometry (IMS-MS/MS), enables complete elucidation of GOS structure. It has been shown that) beta-galactosidase from Aspergillus oryzae has the highest affinity toward formation of beta-(1 - gt 3) or beta-(1 - gt 6) linkages. Additionally, it was observed that the occurrence of different linkages varies during the reaction course, indicating that tailo...ring favorable GOS structures with improved prebiotic activity can be achieved by adequate control of enzymatic synthesis.
Кључне речи:
A. oryzae / galacto-oligosaccharides / structure / IMS-MS/MS / mass spectrometryИзвор:
Journal of Agricultural and Food Chemistry, 2016, 64, 18, 3609-3615Издавач:
- Amer Chemical Soc, Washington
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-MESTD-Basic Research (BR or ON)-172049)
DOI: 10.1021/acs.jafc.6b01293
ISSN: 0021-8561
PubMed: 27109424
WoS: 000375969100016
Scopus: 2-s2.0-84969850263
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Simović, Milica AU - Bezbradica, Dejan AU - Banjanac, Katarina AU - Milivojević, Ana AU - Fanuel, Mathieu AU - Rogniaux, Helene AU - Ropartz, David AU - Veličković, Dušan PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3258 AB - Galacto-oligosaccharides (GOS) represent a diverse group of well-characterized prebiotic ingredients derived from lactose in a reaction catalyzed with beta-galactosidases. Enzymatic transgalactosylation results in a mixture of compounds of various degrees of polymerization and types of linkages. Because structure plays an important role in terms of prebiotic activity, it is of crucial importance to provide an insight into the mechanism of transgalactosylation reaction and occurrence of different types of beta-linkages during GOS synthesis. Our study proved that a novel one-step method, based on ion-mobility spectrometry tandem mass spectrometry (IMS-MS/MS), enables complete elucidation of GOS structure. It has been shown that) beta-galactosidase from Aspergillus oryzae has the highest affinity toward formation of beta-(1 - gt 3) or beta-(1 - gt 6) linkages. Additionally, it was observed that the occurrence of different linkages varies during the reaction course, indicating that tailoring favorable GOS structures with improved prebiotic activity can be achieved by adequate control of enzymatic synthesis. PB - Amer Chemical Soc, Washington T2 - Journal of Agricultural and Food Chemistry T1 - Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry EP - 3615 IS - 18 SP - 3609 VL - 64 DO - 10.1021/acs.jafc.6b01293 ER -
@article{ author = "Simović, Milica and Bezbradica, Dejan and Banjanac, Katarina and Milivojević, Ana and Fanuel, Mathieu and Rogniaux, Helene and Ropartz, David and Veličković, Dušan", year = "2016", abstract = "Galacto-oligosaccharides (GOS) represent a diverse group of well-characterized prebiotic ingredients derived from lactose in a reaction catalyzed with beta-galactosidases. Enzymatic transgalactosylation results in a mixture of compounds of various degrees of polymerization and types of linkages. Because structure plays an important role in terms of prebiotic activity, it is of crucial importance to provide an insight into the mechanism of transgalactosylation reaction and occurrence of different types of beta-linkages during GOS synthesis. Our study proved that a novel one-step method, based on ion-mobility spectrometry tandem mass spectrometry (IMS-MS/MS), enables complete elucidation of GOS structure. It has been shown that) beta-galactosidase from Aspergillus oryzae has the highest affinity toward formation of beta-(1 - gt 3) or beta-(1 - gt 6) linkages. Additionally, it was observed that the occurrence of different linkages varies during the reaction course, indicating that tailoring favorable GOS structures with improved prebiotic activity can be achieved by adequate control of enzymatic synthesis.", publisher = "Amer Chemical Soc, Washington", journal = "Journal of Agricultural and Food Chemistry", title = "Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry", pages = "3615-3609", number = "18", volume = "64", doi = "10.1021/acs.jafc.6b01293" }
Simović, M., Bezbradica, D., Banjanac, K., Milivojević, A., Fanuel, M., Rogniaux, H., Ropartz, D.,& Veličković, D.. (2016). Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry. in Journal of Agricultural and Food Chemistry Amer Chemical Soc, Washington., 64(18), 3609-3615. https://doi.org/10.1021/acs.jafc.6b01293
Simović M, Bezbradica D, Banjanac K, Milivojević A, Fanuel M, Rogniaux H, Ropartz D, Veličković D. Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry. in Journal of Agricultural and Food Chemistry. 2016;64(18):3609-3615. doi:10.1021/acs.jafc.6b01293 .
Simović, Milica, Bezbradica, Dejan, Banjanac, Katarina, Milivojević, Ana, Fanuel, Mathieu, Rogniaux, Helene, Ropartz, David, Veličković, Dušan, "Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry Tandem Mass Spectrometry" in Journal of Agricultural and Food Chemistry, 64, no. 18 (2016):3609-3615, https://doi.org/10.1021/acs.jafc.6b01293 . .