Sugar beet pulp (SBP) and molasses, as an agro industrial waste material, are produced in large amounts annually. Thus, a major challenge nowadays is to develop procedures that could increase the value of the generated waste. In this study, SBP as a support for cell immobilization and molasses as a source of nutrients were used for a dextransucrase (DS) production by Leuconostoc mesenteroides T3. The influence of SBP in native form (SBP-N) and after treatment with NaOH (SBP-NaOH) on DS production was investigated. The optimal medium composition for the maximum DS production was determined by varying the concentration of molasses, SBP, and sucrose. The maximum DS yield of 2.02 U/ml was obtained in the medium with 2.5 % of molasses, 2.5 % SBP-NaOH, and 4 % of sucrose concentration. Scanning electron microscopy (SEM) showed immobilization of Lc. mesenteroides T3 cells onto SBP-NaOH. According to the obtained results, the production of DS on molasses could be improved by using NaOH-treated... SBP as a carrier for whole-cell immobilization. Our study reveals the basis for the development of process for DS production with additional reduction of expenses by using waste materials for obtaining the valuable biotechnological product.
TY - JOUR
AU - Miljković, Miona
AU - Davidović, Slađana
AU - Simović, Milica
AU - Veljović, Đorđe
AU - Mladenović, Dragana
AU - Rajilić-Stojanović, Mirjana
AU - Dimitrijević-Branković, Suzana
PY - 2016
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3290
AB - Sugar beet pulp (SBP) and molasses, as an agro industrial waste material, are produced in large amounts annually. Thus, a major challenge nowadays is to develop procedures that could increase the value of the generated waste. In this study, SBP as a support for cell immobilization and molasses as a source of nutrients were used for a dextransucrase (DS) production by Leuconostoc mesenteroides T3. The influence of SBP in native form (SBP-N) and after treatment with NaOH (SBP-NaOH) on DS production was investigated. The optimal medium composition for the maximum DS production was determined by varying the concentration of molasses, SBP, and sucrose. The maximum DS yield of 2.02 U/ml was obtained in the medium with 2.5 % of molasses, 2.5 % SBP-NaOH, and 4 % of sucrose concentration. Scanning electron microscopy (SEM) showed immobilization of Lc. mesenteroides T3 cells onto SBP-NaOH. According to the obtained results, the production of DS on molasses could be improved by using NaOH-treated SBP as a carrier for whole-cell immobilization. Our study reveals the basis for the development of process for DS production with additional reduction of expenses by using waste materials for obtaining the valuable biotechnological product.
PB - Humana Press Inc, Totowa
T2 - Applied Biochemistry and Biotechnology
T1 - Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses
EP - 1027
IS - 5
SP - 1016
VL - 180
DO - 10.1007/s12010-016-2149-x
ER -
@article{
author = "Miljković, Miona and Davidović, Slađana and Simović, Milica and Veljović, Đorđe and Mladenović, Dragana and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2016",
abstract = "Sugar beet pulp (SBP) and molasses, as an agro industrial waste material, are produced in large amounts annually. Thus, a major challenge nowadays is to develop procedures that could increase the value of the generated waste. In this study, SBP as a support for cell immobilization and molasses as a source of nutrients were used for a dextransucrase (DS) production by Leuconostoc mesenteroides T3. The influence of SBP in native form (SBP-N) and after treatment with NaOH (SBP-NaOH) on DS production was investigated. The optimal medium composition for the maximum DS production was determined by varying the concentration of molasses, SBP, and sucrose. The maximum DS yield of 2.02 U/ml was obtained in the medium with 2.5 % of molasses, 2.5 % SBP-NaOH, and 4 % of sucrose concentration. Scanning electron microscopy (SEM) showed immobilization of Lc. mesenteroides T3 cells onto SBP-NaOH. According to the obtained results, the production of DS on molasses could be improved by using NaOH-treated SBP as a carrier for whole-cell immobilization. Our study reveals the basis for the development of process for DS production with additional reduction of expenses by using waste materials for obtaining the valuable biotechnological product.",
publisher = "Humana Press Inc, Totowa",
journal = "Applied Biochemistry and Biotechnology",
title = "Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses",
pages = "1027-1016",
number = "5",
volume = "180",
doi = "10.1007/s12010-016-2149-x"
}
Miljković, M., Davidović, S., Simović, M., Veljović, Đ., Mladenović, D., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2016). Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses. in Applied Biochemistry and Biotechnology
Humana Press Inc, Totowa., 180(5), 1016-1027.
https://doi.org/10.1007/s12010-016-2149-x
Miljković M, Davidović S, Simović M, Veljović Đ, Mladenović D, Rajilić-Stojanović M, Dimitrijević-Branković S. Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses. in Applied Biochemistry and Biotechnology. 2016;180(5):1016-1027.
doi:10.1007/s12010-016-2149-x .
Miljković, Miona, Davidović, Slađana, Simović, Milica, Veljović, Đorđe, Mladenović, Dragana, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses" in Applied Biochemistry and Biotechnology, 180, no. 5 (2016):1016-1027,
https://doi.org/10.1007/s12010-016-2149-x . .
