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Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses
dc.creator | Miljković, Miona | |
dc.creator | Davidović, Slađana | |
dc.creator | Simović, Milica | |
dc.creator | Veljović, Đorđe | |
dc.creator | Mladenović, Dragana | |
dc.creator | Rajilić-Stojanović, Mirjana | |
dc.creator | Dimitrijević-Branković, Suzana | |
dc.date.accessioned | 2021-03-10T13:04:33Z | |
dc.date.available | 2021-03-10T13:04:33Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0273-2289 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3290 | |
dc.description.abstract | Sugar beet pulp (SBP) and molasses, as an agro industrial waste material, are produced in large amounts annually. Thus, a major challenge nowadays is to develop procedures that could increase the value of the generated waste. In this study, SBP as a support for cell immobilization and molasses as a source of nutrients were used for a dextransucrase (DS) production by Leuconostoc mesenteroides T3. The influence of SBP in native form (SBP-N) and after treatment with NaOH (SBP-NaOH) on DS production was investigated. The optimal medium composition for the maximum DS production was determined by varying the concentration of molasses, SBP, and sucrose. The maximum DS yield of 2.02 U/ml was obtained in the medium with 2.5 % of molasses, 2.5 % SBP-NaOH, and 4 % of sucrose concentration. Scanning electron microscopy (SEM) showed immobilization of Lc. mesenteroides T3 cells onto SBP-NaOH. According to the obtained results, the production of DS on molasses could be improved by using NaOH-treated SBP as a carrier for whole-cell immobilization. Our study reveals the basis for the development of process for DS production with additional reduction of expenses by using waste materials for obtaining the valuable biotechnological product. | en |
dc.publisher | Humana Press Inc, Totowa | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS// | |
dc.rights | restrictedAccess | |
dc.source | Applied Biochemistry and Biotechnology | |
dc.subject | Molasses | en |
dc.subject | Sugar beep pulp | en |
dc.subject | Leuconostoc mesenteroides T3 | en |
dc.subject | Immobilization | en |
dc.subject | Dextransucrase | en |
dc.title | Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 1027 | |
dc.citation.issue | 5 | |
dc.citation.other | 180(5): 1016-1027 | |
dc.citation.rank | M22 | |
dc.citation.spage | 1016 | |
dc.citation.volume | 180 | |
dc.identifier.doi | 10.1007/s12010-016-2149-x | |
dc.identifier.pmid | 27287996 | |
dc.identifier.scopus | 2-s2.0-84973620842 | |
dc.identifier.wos | 000387540700015 | |
dc.type.version | publishedVersion |