In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of... these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice.
TY - JOUR
AU - Takić-Miladinov, Dijana
AU - Tomić, Simonida
AU - Stojanović, Sanja T.
AU - Najdanović, Jelena
AU - Filipović, Jovanka
AU - Trajanović, Miroslav
AU - Najman, Stevo
PY - 2016
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3307
AB - In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice.
PB - University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos
T2 - Materials Research-Ibero-American Journal of Materials
T1 - Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid
EP - 1079
IS - 5
SP - 1070
VL - 19
DO - 10.1590/1980-5373-MR-2016-0222
ER -
@article{
author = "Takić-Miladinov, Dijana and Tomić, Simonida and Stojanović, Sanja T. and Najdanović, Jelena and Filipović, Jovanka and Trajanović, Miroslav and Najman, Stevo",
year = "2016",
abstract = "In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice.",
publisher = "University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos",
journal = "Materials Research-Ibero-American Journal of Materials",
title = "Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid",
pages = "1079-1070",
number = "5",
volume = "19",
doi = "10.1590/1980-5373-MR-2016-0222"
}
Takić-Miladinov, D., Tomić, S., Stojanović, S. T., Najdanović, J., Filipović, J., Trajanović, M.,& Najman, S.. (2016). Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid. in Materials Research-Ibero-American Journal of Materials
University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos., 19(5), 1070-1079.
https://doi.org/10.1590/1980-5373-MR-2016-0222
Takić-Miladinov D, Tomić S, Stojanović ST, Najdanović J, Filipović J, Trajanović M, Najman S. Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid. in Materials Research-Ibero-American Journal of Materials. 2016;19(5):1070-1079.
doi:10.1590/1980-5373-MR-2016-0222 .
Takić-Miladinov, Dijana, Tomić, Simonida, Stojanović, Sanja T., Najdanović, Jelena, Filipović, Jovanka, Trajanović, Miroslav, Najman, Stevo, "Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid" in Materials Research-Ibero-American Journal of Materials, 19, no. 5 (2016):1070-1079,
https://doi.org/10.1590/1980-5373-MR-2016-0222 . .
