Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid
2016
Autori
Takić-Miladinov, DijanaTomić, Simonida
Stojanović, Sanja T.
Najdanović, Jelena
Filipović, Jovanka
Trajanović, Miroslav
Najman, Stevo
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of... these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice.
Ključne reči:
HEMA-based hydrogels / itaconic acid / swelling / genotoxicity / Comet assayIzvor:
Materials Research-Ibero-American Journal of Materials, 2016, 19, 5, 1070-1079Izdavač:
- University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos
Finansiranje / projekti:
- Virtuelni koštano zglobni sistem čoveka i njegova primena u pretkliničkoj i kliničkoj praksi (RS-41017)
- Sinteza i karakterizacija novih funkcionalnih polimera i polimernih nanokompozita (RS-172062)
DOI: 10.1590/1980-5373-MR-2016-0222
ISSN: 1516-1439
WoS: 000385626000014
Scopus: 2-s2.0-84989872169
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Takić-Miladinov, Dijana AU - Tomić, Simonida AU - Stojanović, Sanja T. AU - Najdanović, Jelena AU - Filipović, Jovanka AU - Trajanović, Miroslav AU - Najman, Stevo PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3307 AB - In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice. PB - University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos T2 - Materials Research-Ibero-American Journal of Materials T1 - Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid EP - 1079 IS - 5 SP - 1070 VL - 19 DO - 10.1590/1980-5373-MR-2016-0222 ER -
@article{ author = "Takić-Miladinov, Dijana and Tomić, Simonida and Stojanović, Sanja T. and Najdanović, Jelena and Filipović, Jovanka and Trajanović, Miroslav and Najman, Stevo", year = "2016", abstract = "In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice.", publisher = "University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos", journal = "Materials Research-Ibero-American Journal of Materials", title = "Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid", pages = "1079-1070", number = "5", volume = "19", doi = "10.1590/1980-5373-MR-2016-0222" }
Takić-Miladinov, D., Tomić, S., Stojanović, S. T., Najdanović, J., Filipović, J., Trajanović, M.,& Najman, S.. (2016). Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid. in Materials Research-Ibero-American Journal of Materials University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos., 19(5), 1070-1079. https://doi.org/10.1590/1980-5373-MR-2016-0222
Takić-Miladinov D, Tomić S, Stojanović ST, Najdanović J, Filipović J, Trajanović M, Najman S. Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid. in Materials Research-Ibero-American Journal of Materials. 2016;19(5):1070-1079. doi:10.1590/1980-5373-MR-2016-0222 .
Takić-Miladinov, Dijana, Tomić, Simonida, Stojanović, Sanja T., Najdanović, Jelena, Filipović, Jovanka, Trajanović, Miroslav, Najman, Stevo, "Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid" in Materials Research-Ibero-American Journal of Materials, 19, no. 5 (2016):1070-1079, https://doi.org/10.1590/1980-5373-MR-2016-0222 . .