Приказ основних података о документу

dc.creatorBanjanac, Katarina
dc.creatorMihailović, Mladen
dc.creatorPrlainović, Nevena
dc.creatorĆorović, Marija
dc.creatorSimović, Milica
dc.creatorMarinković, Aleksandar
dc.creatorBezbradica, Dejan
dc.date.accessioned2021-03-10T13:06:44Z
dc.date.available2021-03-10T13:06:44Z
dc.date.issued2016
dc.identifier.issn0268-2575
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3323
dc.description.abstractBACKGROUND: In this study, in order to obtain the best support for the immobilization of lipase from Candida rugosa (CRL), the procedure for chemical modification of fumed silica nanoparticles (FNS) with (3-Glycidyloxypropyl) trimethoxysilane (GOPTMS) was optimized by varying the amount of GOPTMS used and the duration of support modification. To evaluate the prospects of chemical modification of FNS surface on the immobilization process, the activity and thermal stability of lipase immobilized on GFNS was compared with lipase immobilized on FNS and cyanuric chloride functionalized amino silica (CCAFNS). RESULTS: The epoxy functionalized silica nanoparticles (GFNS) with 400-500 mu mol of introduced epoxy groups per g of support were the most efficient, since immobilized preparations in 1 mol L-1 buffer had the highest activity of approximately 1495 IU g(-1) and a loading capacity of 294 mg g(-1). Lipase immobilized on GFNS exhibited 1.5-fold higher hydrolytic activity and 2-fold higher esterification activity in the synthesis of aroma esters than lipase immobilized on CCAFNS, while its thermal stability was similar to CCAFNS preparations. CONCLUSION: These results indicated that the type of modification of the FNS surface has an influence on the specificity of the immobilized lipase, since lipase immobilized on GFNS showed improved properties for ester synthesis which is a promising area for immobilized lipase application.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172013/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45019/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Chemical Technology and Biotechnology
dc.subjectbiotechnologyen
dc.subjectbiocatalysisen
dc.subjectlipaseen
dc.subjectimmobilizationen
dc.titleEpoxy-silanization - tool for improvement of silica nanoparticles as support for lipase immobilization with respect to esterification activityen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage2663
dc.citation.issue10
dc.citation.other91(10): 2654-2663
dc.citation.rankM21
dc.citation.spage2654
dc.citation.volume91
dc.identifier.doi10.1002/jctb.4870
dc.identifier.scopus2-s2.0-84953294965
dc.identifier.wos000388291900012
dc.type.versionpublishedVersion


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Приказ основних података о документу