TechnoRep - Faculty of Technology and Metallurgy Repository
University of Belgrade - Faculty of Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   TechnoRep
  • Tehnološko-metalurški fakultet
  • Radovi istraživača / Researchers’ publications (TMF)
  • View Item
  •   TechnoRep
  • Tehnološko-metalurški fakultet
  • Radovi istraživača / Researchers’ publications (TMF)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization

Thumbnail
2016
3348.pdf (1.373Mb)
Authors
Prlainović, Nevena
Bezbradica, Dejan
Rogan, Jelena
Uskoković, Petar
Mijin, Dušan
Marinković, Aleksandar
article (publishedVersion)
Metadata
Show full item record
Abstract
This work examines two approaches for immobilization of lipase from Candida rugosa on oxidized multi-walled carbon nanotubes (o-MWCNTs). One method included the presence of activating agents to promote covalent bonding and the other the adsorption on o-MWCNTs to elucidate if non-specific bonding on the o-MWCNTs surface exists. The influence of the immobilization time and initial enzyme concentration on protein loading and the expressed lypolitic activity of the immobilized preparation were investigated. The results showed that the enzyme adsorbs on o-MWCNTs in a maximal amount of 37 mu g mg(-1) CNTs, while the attached amount was more than 2-times higher under covalent promoting conditions (80 mu g mg(-1) CNTs). Furthermore, similar trends were observed for the lypolitic activity, whereby preparations obtained under covalent promoting conditions had almost 3-times higher activity (560 IU g(-1) of immobilized enzyme). In addition, immobilization of the enzyme was confirmed by Fourier tr...ansformation infrared spectroscopy and thermogravimetric analysis.

Keywords:
Candida rugosa lipase / Enzyme immobilization / Carbon nanotubes / o-MWCNT / Carbodiimide chemistry
Source:
Comptes Rendus Chimie, 2016, 19, 3, 363-370
Publisher:
  • Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
Funding / projects:
  • info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172013/RS// (RS-172013)
  • info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// (RS-46010)
  • info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// (RS-172049)

DOI: 10.1016/j.crci.2015.10.008

ISSN: 1631-0748

WoS: 000385784900014

Scopus: 2-s2.0-84955495328
[ Google Scholar ]
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3351
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet

DSpace software copyright © 2002-2015  DuraSpace
About TechnoRep | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About TechnoRep | Send Feedback

OpenAIRERCUB