Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization

2016
Authors
Prlainović, Nevena
Bezbradica, Dejan

Rogan, Jelena

Uskoković, Petar

Mijin, Dušan

Marinković, Aleksandar

Article (Published version)

Metadata
Show full item recordAbstract
This work examines two approaches for immobilization of lipase from Candida rugosa on oxidized multi-walled carbon nanotubes (o-MWCNTs). One method included the presence of activating agents to promote covalent bonding and the other the adsorption on o-MWCNTs to elucidate if non-specific bonding on the o-MWCNTs surface exists. The influence of the immobilization time and initial enzyme concentration on protein loading and the expressed lypolitic activity of the immobilized preparation were investigated. The results showed that the enzyme adsorbs on o-MWCNTs in a maximal amount of 37 mu g mg(-1) CNTs, while the attached amount was more than 2-times higher under covalent promoting conditions (80 mu g mg(-1) CNTs). Furthermore, similar trends were observed for the lypolitic activity, whereby preparations obtained under covalent promoting conditions had almost 3-times higher activity (560 IU g(-1) of immobilized enzyme). In addition, immobilization of the enzyme was confirmed by Fourier tr...ansformation infrared spectroscopy and thermogravimetric analysis.
Keywords:
Candida rugosa lipase / Enzyme immobilization / Carbon nanotubes / o-MWCNT / Carbodiimide chemistrySource:
Comptes Rendus Chimie, 2016, 19, 3, 363-370Publisher:
- Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
Funding / projects:
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)
DOI: 10.1016/j.crci.2015.10.008
ISSN: 1631-0748
WoS: 000385784900014
Scopus: 2-s2.0-84955495328
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Prlainović, Nevena AU - Bezbradica, Dejan AU - Rogan, Jelena AU - Uskoković, Petar AU - Mijin, Dušan AU - Marinković, Aleksandar PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3351 AB - This work examines two approaches for immobilization of lipase from Candida rugosa on oxidized multi-walled carbon nanotubes (o-MWCNTs). One method included the presence of activating agents to promote covalent bonding and the other the adsorption on o-MWCNTs to elucidate if non-specific bonding on the o-MWCNTs surface exists. The influence of the immobilization time and initial enzyme concentration on protein loading and the expressed lypolitic activity of the immobilized preparation were investigated. The results showed that the enzyme adsorbs on o-MWCNTs in a maximal amount of 37 mu g mg(-1) CNTs, while the attached amount was more than 2-times higher under covalent promoting conditions (80 mu g mg(-1) CNTs). Furthermore, similar trends were observed for the lypolitic activity, whereby preparations obtained under covalent promoting conditions had almost 3-times higher activity (560 IU g(-1) of immobilized enzyme). In addition, immobilization of the enzyme was confirmed by Fourier transformation infrared spectroscopy and thermogravimetric analysis. PB - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux T2 - Comptes Rendus Chimie T1 - Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization EP - 370 IS - 3 SP - 363 VL - 19 DO - 10.1016/j.crci.2015.10.008 ER -
@article{ author = "Prlainović, Nevena and Bezbradica, Dejan and Rogan, Jelena and Uskoković, Petar and Mijin, Dušan and Marinković, Aleksandar", year = "2016", abstract = "This work examines two approaches for immobilization of lipase from Candida rugosa on oxidized multi-walled carbon nanotubes (o-MWCNTs). One method included the presence of activating agents to promote covalent bonding and the other the adsorption on o-MWCNTs to elucidate if non-specific bonding on the o-MWCNTs surface exists. The influence of the immobilization time and initial enzyme concentration on protein loading and the expressed lypolitic activity of the immobilized preparation were investigated. The results showed that the enzyme adsorbs on o-MWCNTs in a maximal amount of 37 mu g mg(-1) CNTs, while the attached amount was more than 2-times higher under covalent promoting conditions (80 mu g mg(-1) CNTs). Furthermore, similar trends were observed for the lypolitic activity, whereby preparations obtained under covalent promoting conditions had almost 3-times higher activity (560 IU g(-1) of immobilized enzyme). In addition, immobilization of the enzyme was confirmed by Fourier transformation infrared spectroscopy and thermogravimetric analysis.", publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux", journal = "Comptes Rendus Chimie", title = "Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization", pages = "370-363", number = "3", volume = "19", doi = "10.1016/j.crci.2015.10.008" }
Prlainović, N., Bezbradica, D., Rogan, J., Uskoković, P., Mijin, D.,& Marinković, A.. (2016). Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization. in Comptes Rendus Chimie Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 19(3), 363-370. https://doi.org/10.1016/j.crci.2015.10.008
Prlainović N, Bezbradica D, Rogan J, Uskoković P, Mijin D, Marinković A. Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization. in Comptes Rendus Chimie. 2016;19(3):363-370. doi:10.1016/j.crci.2015.10.008 .
Prlainović, Nevena, Bezbradica, Dejan, Rogan, Jelena, Uskoković, Petar, Mijin, Dušan, Marinković, Aleksandar, "Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization" in Comptes Rendus Chimie, 19, no. 3 (2016):363-370, https://doi.org/10.1016/j.crci.2015.10.008 . .