Приказ основних података о документу

dc.creatorPrlainović, Nevena
dc.creatorBezbradica, Dejan
dc.creatorRogan, Jelena
dc.creatorUskoković, Petar
dc.creatorMijin, Dušan
dc.creatorMarinković, Aleksandar
dc.date.accessioned2021-03-10T13:08:34Z
dc.date.available2021-03-10T13:08:34Z
dc.date.issued2016
dc.identifier.issn1631-0748
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3351
dc.description.abstractThis work examines two approaches for immobilization of lipase from Candida rugosa on oxidized multi-walled carbon nanotubes (o-MWCNTs). One method included the presence of activating agents to promote covalent bonding and the other the adsorption on o-MWCNTs to elucidate if non-specific bonding on the o-MWCNTs surface exists. The influence of the immobilization time and initial enzyme concentration on protein loading and the expressed lypolitic activity of the immobilized preparation were investigated. The results showed that the enzyme adsorbs on o-MWCNTs in a maximal amount of 37 mu g mg(-1) CNTs, while the attached amount was more than 2-times higher under covalent promoting conditions (80 mu g mg(-1) CNTs). Furthermore, similar trends were observed for the lypolitic activity, whereby preparations obtained under covalent promoting conditions had almost 3-times higher activity (560 IU g(-1) of immobilized enzyme). In addition, immobilization of the enzyme was confirmed by Fourier transformation infrared spectroscopy and thermogravimetric analysis.en
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172013/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsopenAccess
dc.sourceComptes Rendus Chimie
dc.subjectCandida rugosa lipaseen
dc.subjectEnzyme immobilizationen
dc.subjectCarbon nanotubesen
dc.subjecto-MWCNTen
dc.subjectCarbodiimide chemistryen
dc.titleSurface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilizationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage370
dc.citation.issue3
dc.citation.other19(3): 363-370
dc.citation.rankM22
dc.citation.spage363
dc.citation.volume19
dc.identifier.doi10.1016/j.crci.2015.10.008
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/1273/3348.pdf
dc.identifier.scopus2-s2.0-84955495328
dc.identifier.wos000385784900014
dc.type.versionpublishedVersion


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Приказ основних података о документу