In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management
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2016
Authors
Vuković, JovanaBabić, Marija
Antić, Katarina
Filipović, Jovanka M.
Stojanović, Sanja T.
Najman, Stevo
Tomić, Simonida
Article (Published version)
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Show full item recordAbstract
In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infec...tion in wound bed.
Keywords:
Polymers / Mechanical properties / Transport properties / BiomaterialsSource:
Materials Chemistry and Physics, 2016, 175, 158-163Publisher:
- Elsevier Science Sa, Lausanne
Funding / projects:
- Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-172062)
- Chemical and structural designing of nanomaterials for application in medicine and tissue engineering (RS-172026)
DOI: 10.1016/j.matchemphys.2016.03.009
ISSN: 0254-0584
WoS: 000374612000020
Scopus: 2-s2.0-84977934865
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Tehnološko-metalurški fakultetTY - JOUR AU - Vuković, Jovana AU - Babić, Marija AU - Antić, Katarina AU - Filipović, Jovanka M. AU - Stojanović, Sanja T. AU - Najman, Stevo AU - Tomić, Simonida PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3352 AB - In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed. PB - Elsevier Science Sa, Lausanne T2 - Materials Chemistry and Physics T1 - In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management EP - 163 SP - 158 VL - 175 DO - 10.1016/j.matchemphys.2016.03.009 ER -
@article{ author = "Vuković, Jovana and Babić, Marija and Antić, Katarina and Filipović, Jovanka M. and Stojanović, Sanja T. and Najman, Stevo and Tomić, Simonida", year = "2016", abstract = "In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed.", publisher = "Elsevier Science Sa, Lausanne", journal = "Materials Chemistry and Physics", title = "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management", pages = "163-158", volume = "175", doi = "10.1016/j.matchemphys.2016.03.009" }
Vuković, J., Babić, M., Antić, K., Filipović, J. M., Stojanović, S. T., Najman, S.,& Tomić, S.. (2016). In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics Elsevier Science Sa, Lausanne., 175, 158-163. https://doi.org/10.1016/j.matchemphys.2016.03.009
Vuković J, Babić M, Antić K, Filipović JM, Stojanović ST, Najman S, Tomić S. In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics. 2016;175:158-163. doi:10.1016/j.matchemphys.2016.03.009 .
Vuković, Jovana, Babić, Marija, Antić, Katarina, Filipović, Jovanka M., Stojanović, Sanja T., Najman, Stevo, Tomić, Simonida, "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management" in Materials Chemistry and Physics, 175 (2016):158-163, https://doi.org/10.1016/j.matchemphys.2016.03.009 . .