In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infec...tion in wound bed.
Кључне речи:
Polymers / Mechanical properties / Transport properties / Biomaterials
Извор:
Materials Chemistry and Physics, 2016, 175, 158-163
TY - JOUR
AU - Vuković, Jovana
AU - Babić, Marija
AU - Antić, Katarina
AU - Filipović, Jovanka M.
AU - Stojanović, Sanja T.
AU - Najman, Stevo
AU - Tomić, Simonida
PY - 2016
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3352
AB - In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed.
PB - Elsevier Science Sa, Lausanne
T2 - Materials Chemistry and Physics
T1 - In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management
EP - 163
SP - 158
VL - 175
DO - 10.1016/j.matchemphys.2016.03.009
ER -
@article{
author = "Vuković, Jovana and Babić, Marija and Antić, Katarina and Filipović, Jovanka M. and Stojanović, Sanja T. and Najman, Stevo and Tomić, Simonida",
year = "2016",
abstract = "In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Materials Chemistry and Physics",
title = "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management",
pages = "163-158",
volume = "175",
doi = "10.1016/j.matchemphys.2016.03.009"
}
Vuković, J., Babić, M., Antić, K., Filipović, J. M., Stojanović, S. T., Najman, S.,& Tomić, S.. (2016). In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics
Elsevier Science Sa, Lausanne., 175, 158-163.
https://doi.org/10.1016/j.matchemphys.2016.03.009
Vuković J, Babić M, Antić K, Filipović JM, Stojanović ST, Najman S, Tomić S. In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics. 2016;175:158-163.
doi:10.1016/j.matchemphys.2016.03.009 .
Vuković, Jovana, Babić, Marija, Antić, Katarina, Filipović, Jovanka M., Stojanović, Sanja T., Najman, Stevo, Tomić, Simonida, "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management" in Materials Chemistry and Physics, 175 (2016):158-163,
https://doi.org/10.1016/j.matchemphys.2016.03.009 . .
