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Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1

Authorized Users Only
2016
Authors
Mihajlovski, Katarina
Radovanović, Neda
Veljović, Đorđe
Šiler-Marinković, Slavica
Dimitrijević-Branković, Suzana
Article (Published version)
Metadata
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Abstract
Molasses and sugar beet pulp, by-products in sugar industry, are produced in large amounts annually which create disposal problems. High sugar content in sugar beet molasses enables its use for fermentations while sugar beet pulp represents an interesting cheap raw material source for enzyme production. beta-amylase production by a new strain Paenibacillus chitinolyticus CKS1 was performed using a molasses and sugar beet pulp. Two different pretreatments of SBP were investigated while scanning electron microscopy (SEM) showed immobilization of P. chitinolyticus CKS1 cells onto alkali treated SBP. The statistical model was design via Central Composite Design (CCD) under the Response Surface Methodology (RSM) using three variables (inoculum concentration, time of incubation and sugar beet pulp concentration) for obtaining maximum beta-amylase activity. An approximately 3.66-fold increase in beta-amylase production was achieved in the optimized medium as compared with the nonoptimized med...ium. A maximum beta-amylase production 2.237 U/ml was achieved under optimal conditions inoculum concentration 10%, incubation time 83.07 h and SBP concentration 3%. A high performance liquid chromatography showed that P. chitinolyticus CKS1 hydrolyzed starch to form maltose as a major product. These results indicate that beta-amylase production could be improved using these kind of processes. Also, taking into consideration cheap substrates, economic side of the process is justified.

Keywords:
Sugar beet pulp / Paenibacillus chitinolyticus CKS1 / Immobilization / beta-Amylase production production / response surface methodology (RSM) / Maltose
Source:
Industrial Crops and Products, 2016, 80, 115-122
Publisher:
  • Elsevier, Amsterdam
Funding / projects:
  • Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-31035)

DOI: 10.1016/j.indcrop.2015.11.025

ISSN: 0926-6690

WoS: 000370107600015

Scopus: 2-s2.0-84947976516
[ Google Scholar ]
20
18
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3390
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
  • Radovi istraživača (Inovacioni centar) / Researchers’ publications (Innovation Centre)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Radovanović, Neda
AU  - Veljović, Đorđe
AU  - Šiler-Marinković, Slavica
AU  - Dimitrijević-Branković, Suzana
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3390
AB  - Molasses and sugar beet pulp, by-products in sugar industry, are produced in large amounts annually which create disposal problems. High sugar content in sugar beet molasses enables its use for fermentations while sugar beet pulp represents an interesting cheap raw material source for enzyme production. beta-amylase production by a new strain Paenibacillus chitinolyticus CKS1 was performed using a molasses and sugar beet pulp. Two different pretreatments of SBP were investigated while scanning electron microscopy (SEM) showed immobilization of P. chitinolyticus CKS1 cells onto alkali treated SBP. The statistical model was design via Central Composite Design (CCD) under the Response Surface Methodology (RSM) using three variables (inoculum concentration, time of incubation and sugar beet pulp concentration) for obtaining maximum beta-amylase activity. An approximately 3.66-fold increase in beta-amylase production was achieved in the optimized medium as compared with the nonoptimized medium. A maximum beta-amylase production 2.237 U/ml was achieved under optimal conditions inoculum concentration 10%, incubation time 83.07 h and SBP concentration 3%. A high performance liquid chromatography showed that P. chitinolyticus CKS1 hydrolyzed starch to form maltose as a major product. These results indicate that beta-amylase production could be improved using these kind of processes. Also, taking into consideration cheap substrates, economic side of the process is justified.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1
EP  - 122
SP  - 115
VL  - 80
DO  - 10.1016/j.indcrop.2015.11.025
ER  - 
@article{
author = "Mihajlovski, Katarina and Radovanović, Neda and Veljović, Đorđe and Šiler-Marinković, Slavica and Dimitrijević-Branković, Suzana",
year = "2016",
abstract = "Molasses and sugar beet pulp, by-products in sugar industry, are produced in large amounts annually which create disposal problems. High sugar content in sugar beet molasses enables its use for fermentations while sugar beet pulp represents an interesting cheap raw material source for enzyme production. beta-amylase production by a new strain Paenibacillus chitinolyticus CKS1 was performed using a molasses and sugar beet pulp. Two different pretreatments of SBP were investigated while scanning electron microscopy (SEM) showed immobilization of P. chitinolyticus CKS1 cells onto alkali treated SBP. The statistical model was design via Central Composite Design (CCD) under the Response Surface Methodology (RSM) using three variables (inoculum concentration, time of incubation and sugar beet pulp concentration) for obtaining maximum beta-amylase activity. An approximately 3.66-fold increase in beta-amylase production was achieved in the optimized medium as compared with the nonoptimized medium. A maximum beta-amylase production 2.237 U/ml was achieved under optimal conditions inoculum concentration 10%, incubation time 83.07 h and SBP concentration 3%. A high performance liquid chromatography showed that P. chitinolyticus CKS1 hydrolyzed starch to form maltose as a major product. These results indicate that beta-amylase production could be improved using these kind of processes. Also, taking into consideration cheap substrates, economic side of the process is justified.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1",
pages = "122-115",
volume = "80",
doi = "10.1016/j.indcrop.2015.11.025"
}
Mihajlovski, K., Radovanović, N., Veljović, Đ., Šiler-Marinković, S.,& Dimitrijević-Branković, S.. (2016). Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1. in Industrial Crops and Products
Elsevier, Amsterdam., 80, 115-122.
https://doi.org/10.1016/j.indcrop.2015.11.025
Mihajlovski K, Radovanović N, Veljović Đ, Šiler-Marinković S, Dimitrijević-Branković S. Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1. in Industrial Crops and Products. 2016;80:115-122.
doi:10.1016/j.indcrop.2015.11.025 .
Mihajlovski, Katarina, Radovanović, Neda, Veljović, Đorđe, Šiler-Marinković, Slavica, Dimitrijević-Branković, Suzana, "Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1" in Industrial Crops and Products, 80 (2016):115-122,
https://doi.org/10.1016/j.indcrop.2015.11.025 . .

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