Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1
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2016
Authors
Mihajlovski, Katarina
Radovanović, Neda

Veljović, Đorđe

Šiler-Marinković, Slavica

Dimitrijević-Branković, Suzana

Article (Published version)

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Molasses and sugar beet pulp, by-products in sugar industry, are produced in large amounts annually which create disposal problems. High sugar content in sugar beet molasses enables its use for fermentations while sugar beet pulp represents an interesting cheap raw material source for enzyme production. beta-amylase production by a new strain Paenibacillus chitinolyticus CKS1 was performed using a molasses and sugar beet pulp. Two different pretreatments of SBP were investigated while scanning electron microscopy (SEM) showed immobilization of P. chitinolyticus CKS1 cells onto alkali treated SBP. The statistical model was design via Central Composite Design (CCD) under the Response Surface Methodology (RSM) using three variables (inoculum concentration, time of incubation and sugar beet pulp concentration) for obtaining maximum beta-amylase activity. An approximately 3.66-fold increase in beta-amylase production was achieved in the optimized medium as compared with the nonoptimized med...ium. A maximum beta-amylase production 2.237 U/ml was achieved under optimal conditions inoculum concentration 10%, incubation time 83.07 h and SBP concentration 3%. A high performance liquid chromatography showed that P. chitinolyticus CKS1 hydrolyzed starch to form maltose as a major product. These results indicate that beta-amylase production could be improved using these kind of processes. Also, taking into consideration cheap substrates, economic side of the process is justified.
Keywords:
Sugar beet pulp / Paenibacillus chitinolyticus CKS1 / Immobilization / beta-Amylase production production / response surface methodology (RSM) / MaltoseSource:
Industrial Crops and Products, 2016, 80, 115-122Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-31035)
DOI: 10.1016/j.indcrop.2015.11.025
ISSN: 0926-6690
WoS: 000370107600015
Scopus: 2-s2.0-84947976516
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Tehnološko-metalurški fakultetTY - JOUR AU - Mihajlovski, Katarina AU - Radovanović, Neda AU - Veljović, Đorđe AU - Šiler-Marinković, Slavica AU - Dimitrijević-Branković, Suzana PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3390 AB - Molasses and sugar beet pulp, by-products in sugar industry, are produced in large amounts annually which create disposal problems. High sugar content in sugar beet molasses enables its use for fermentations while sugar beet pulp represents an interesting cheap raw material source for enzyme production. beta-amylase production by a new strain Paenibacillus chitinolyticus CKS1 was performed using a molasses and sugar beet pulp. Two different pretreatments of SBP were investigated while scanning electron microscopy (SEM) showed immobilization of P. chitinolyticus CKS1 cells onto alkali treated SBP. The statistical model was design via Central Composite Design (CCD) under the Response Surface Methodology (RSM) using three variables (inoculum concentration, time of incubation and sugar beet pulp concentration) for obtaining maximum beta-amylase activity. An approximately 3.66-fold increase in beta-amylase production was achieved in the optimized medium as compared with the nonoptimized medium. A maximum beta-amylase production 2.237 U/ml was achieved under optimal conditions inoculum concentration 10%, incubation time 83.07 h and SBP concentration 3%. A high performance liquid chromatography showed that P. chitinolyticus CKS1 hydrolyzed starch to form maltose as a major product. These results indicate that beta-amylase production could be improved using these kind of processes. Also, taking into consideration cheap substrates, economic side of the process is justified. PB - Elsevier, Amsterdam T2 - Industrial Crops and Products T1 - Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1 EP - 122 SP - 115 VL - 80 DO - 10.1016/j.indcrop.2015.11.025 ER -
@article{ author = "Mihajlovski, Katarina and Radovanović, Neda and Veljović, Đorđe and Šiler-Marinković, Slavica and Dimitrijević-Branković, Suzana", year = "2016", abstract = "Molasses and sugar beet pulp, by-products in sugar industry, are produced in large amounts annually which create disposal problems. High sugar content in sugar beet molasses enables its use for fermentations while sugar beet pulp represents an interesting cheap raw material source for enzyme production. beta-amylase production by a new strain Paenibacillus chitinolyticus CKS1 was performed using a molasses and sugar beet pulp. Two different pretreatments of SBP were investigated while scanning electron microscopy (SEM) showed immobilization of P. chitinolyticus CKS1 cells onto alkali treated SBP. The statistical model was design via Central Composite Design (CCD) under the Response Surface Methodology (RSM) using three variables (inoculum concentration, time of incubation and sugar beet pulp concentration) for obtaining maximum beta-amylase activity. An approximately 3.66-fold increase in beta-amylase production was achieved in the optimized medium as compared with the nonoptimized medium. A maximum beta-amylase production 2.237 U/ml was achieved under optimal conditions inoculum concentration 10%, incubation time 83.07 h and SBP concentration 3%. A high performance liquid chromatography showed that P. chitinolyticus CKS1 hydrolyzed starch to form maltose as a major product. These results indicate that beta-amylase production could be improved using these kind of processes. Also, taking into consideration cheap substrates, economic side of the process is justified.", publisher = "Elsevier, Amsterdam", journal = "Industrial Crops and Products", title = "Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1", pages = "122-115", volume = "80", doi = "10.1016/j.indcrop.2015.11.025" }
Mihajlovski, K., Radovanović, N., Veljović, Đ., Šiler-Marinković, S.,& Dimitrijević-Branković, S.. (2016). Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1. in Industrial Crops and Products Elsevier, Amsterdam., 80, 115-122. https://doi.org/10.1016/j.indcrop.2015.11.025
Mihajlovski K, Radovanović N, Veljović Đ, Šiler-Marinković S, Dimitrijević-Branković S. Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1. in Industrial Crops and Products. 2016;80:115-122. doi:10.1016/j.indcrop.2015.11.025 .
Mihajlovski, Katarina, Radovanović, Neda, Veljović, Đorđe, Šiler-Marinković, Slavica, Dimitrijević-Branković, Suzana, "Improved beta-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1" in Industrial Crops and Products, 80 (2016):115-122, https://doi.org/10.1016/j.indcrop.2015.11.025 . .