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Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization

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2016
3411.pdf (827.1Kb)
Authors
Buntić, Aneta
Pavlović, Marija D.
Antonović, Dušan
Šiler-Marinković, Slavica
Dimitrijević-Branković, Suzana
article (publishedVersion)
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Abstract
This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme app...lication. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.

Keywords:
Biotechnology / Food science / Materials science / Bioengineering / Ecology
Source:
Heliyon, 2016, 2, 8
Publisher:
  • Elsevier Sci Ltd, Oxford
Funding / projects:
  • info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS// (RS-31035)

DOI: 10.1016/j.heliyon.2016.e00146

ISSN: 2405-8440

PubMed: 27626091

WoS: 000432010800002

Scopus: 2-s2.0-84989227454
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URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3414
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  • Radovi istraživača / Researchers’ publications (TMF)
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Tehnološko-metalurški fakultet

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