Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization

2016
Autori
Buntić, Aneta
Pavlović, Marija D.
Antonović, Dušan
Šiler-Marinković, Slavica

Dimitrijević-Branković, Suzana

Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme app...lication. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.
Ključne reči:
Biotechnology / Food science / Materials science / Bioengineering / EcologyIzvor:
Heliyon, 2016, 2, 8Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
DOI: 10.1016/j.heliyon.2016.e00146
ISSN: 2405-8440
PubMed: 27626091
WoS: 000432010800002
Scopus: 2-s2.0-84989227454
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Buntić, Aneta AU - Pavlović, Marija D. AU - Antonović, Dušan AU - Šiler-Marinković, Slavica AU - Dimitrijević-Branković, Suzana PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3414 AB - This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme application. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization. PB - Elsevier Sci Ltd, Oxford T2 - Heliyon T1 - Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization IS - 8 VL - 2 DO - 10.1016/j.heliyon.2016.e00146 ER -
@article{ author = "Buntić, Aneta and Pavlović, Marija D. and Antonović, Dušan and Šiler-Marinković, Slavica and Dimitrijević-Branković, Suzana", year = "2016", abstract = "This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme application. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Heliyon", title = "Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization", number = "8", volume = "2", doi = "10.1016/j.heliyon.2016.e00146" }
Buntić, A., Pavlović, M. D., Antonović, D., Šiler-Marinković, S.,& Dimitrijević-Branković, S.. (2016). Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization. in Heliyon Elsevier Sci Ltd, Oxford., 2(8). https://doi.org/10.1016/j.heliyon.2016.e00146
Buntić A, Pavlović MD, Antonović D, Šiler-Marinković S, Dimitrijević-Branković S. Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization. in Heliyon. 2016;2(8). doi:10.1016/j.heliyon.2016.e00146 .
Buntić, Aneta, Pavlović, Marija D., Antonović, Dušan, Šiler-Marinković, Slavica, Dimitrijević-Branković, Suzana, "Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization" in Heliyon, 2, no. 8 (2016), https://doi.org/10.1016/j.heliyon.2016.e00146 . .