Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization

2016
Autori
Buntić, Aneta
Pavlović, Marija D.
Antonović, Dušan
Šiler-Marinković, Slavica

Dimitrijević-Branković, Suzana

article (publishedVersion)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme app...lication. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.
Ključne reči:
Biotechnology / Food science / Materials science / Bioengineering / EcologyIzvor:
Heliyon, 2016, 2, 8Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
DOI: 10.1016/j.heliyon.2016.e00146
ISSN: 2405-8440
PubMed: 27626091