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Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization

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2016
3411.pdf (827.1Kb)
Autori
Buntić, Aneta
Pavlović, Marija D.
Antonović, Dušan
Šiler-Marinković, Slavica
Dimitrijević-Branković, Suzana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme app...lication. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.

Ključne reči:
Biotechnology / Food science / Materials science / Bioengineering / Ecology
Izvor:
Heliyon, 2016, 2, 8
Izdavač:
  • Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
  • Primena biotehnoloških metoda u održivom iskorišćenju nus-proizvoda agroindustrije (RS-31035)

DOI: 10.1016/j.heliyon.2016.e00146

ISSN: 2405-8440

PubMed: 27626091

WoS: 000432010800002

Scopus: 2-s2.0-84989227454
[ Google Scholar ]
15
10
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3414
Kolekcije
  • Radovi istraživača / Researchers’ publications (TMF)
Institucija/grupa
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Buntić, Aneta
AU  - Pavlović, Marija D.
AU  - Antonović, Dušan
AU  - Šiler-Marinković, Slavica
AU  - Dimitrijević-Branković, Suzana
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3414
AB  - This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme application. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.
PB  - Elsevier Sci Ltd, Oxford
T2  - Heliyon
T1  - Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization
IS  - 8
VL  - 2
DO  - 10.1016/j.heliyon.2016.e00146
ER  - 
@article{
author = "Buntić, Aneta and Pavlović, Marija D. and Antonović, Dušan and Šiler-Marinković, Slavica and Dimitrijević-Branković, Suzana",
year = "2016",
abstract = "This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme application. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Heliyon",
title = "Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization",
number = "8",
volume = "2",
doi = "10.1016/j.heliyon.2016.e00146"
}
Buntić, A., Pavlović, M. D., Antonović, D., Šiler-Marinković, S.,& Dimitrijević-Branković, S.. (2016). Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization. in Heliyon
Elsevier Sci Ltd, Oxford., 2(8).
https://doi.org/10.1016/j.heliyon.2016.e00146
Buntić A, Pavlović MD, Antonović D, Šiler-Marinković S, Dimitrijević-Branković S. Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization. in Heliyon. 2016;2(8).
doi:10.1016/j.heliyon.2016.e00146 .
Buntić, Aneta, Pavlović, Marija D., Antonović, Dušan, Šiler-Marinković, Slavica, Dimitrijević-Branković, Suzana, "Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization" in Heliyon, 2, no. 8 (2016),
https://doi.org/10.1016/j.heliyon.2016.e00146 . .

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