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dc.creatorBuntić, Aneta
dc.creatorPavlović, Marija D.
dc.creatorAntonović, Dušan
dc.creatorŠiler-Marinković, Slavica
dc.creatorDimitrijević-Branković, Suzana
dc.date.accessioned2021-03-10T13:12:39Z
dc.date.available2021-03-10T13:12:39Z
dc.date.issued2016
dc.identifier.issn2405-8440
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3414
dc.description.abstractThis study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme application. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceHeliyon
dc.subjectBiotechnologyen
dc.subjectFood scienceen
dc.subjectMaterials scienceen
dc.subjectBioengineeringen
dc.subjectEcologyen
dc.titleUtilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilizationen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.issue8
dc.citation.other2(8): -
dc.citation.volume2
dc.identifier.doi10.1016/j.heliyon.2016.e00146
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/1323/3411.pdf
dc.identifier.pmid27626091
dc.identifier.rcubconv_5578
dc.identifier.scopus2-s2.0-84989227454
dc.identifier.wos000432010800002
dc.type.versionpublishedVersion


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