Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w(-1)) polyethylene glycol 4000/15% (w w(-1)) KH2PO4/70% (w w(-1)) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery.
TY - JOUR
AU - Antov, Mirjana
AU - Ivetić, Darjana Z.
AU - Knežević-Jugović, Zorica
PY - 2016
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3438
AB - Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w(-1)) polyethylene glycol 4000/15% (w w(-1)) KH2PO4/70% (w w(-1)) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery.
PB - Taylor & Francis Inc, Philadelphia
T2 - Separation Science and Technology
T1 - Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction
EP - 628
IS - 4
SP - 622
VL - 51
DO - 10.1080/01496395.2015.1117106
ER -
@article{
author = "Antov, Mirjana and Ivetić, Darjana Z. and Knežević-Jugović, Zorica",
year = "2016",
abstract = "Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w(-1)) polyethylene glycol 4000/15% (w w(-1)) KH2PO4/70% (w w(-1)) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Separation Science and Technology",
title = "Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction",
pages = "628-622",
number = "4",
volume = "51",
doi = "10.1080/01496395.2015.1117106"
}
Antov, M., Ivetić, D. Z.,& Knežević-Jugović, Z.. (2016). Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction. in Separation Science and Technology
Taylor & Francis Inc, Philadelphia., 51(4), 622-628.
https://doi.org/10.1080/01496395.2015.1117106
Antov M, Ivetić DZ, Knežević-Jugović Z. Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction. in Separation Science and Technology. 2016;51(4):622-628.
doi:10.1080/01496395.2015.1117106 .
Antov, Mirjana, Ivetić, Darjana Z., Knežević-Jugović, Zorica, "Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction" in Separation Science and Technology, 51, no. 4 (2016):622-628,
https://doi.org/10.1080/01496395.2015.1117106 . .
