The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
TY - JOUR
AU - Lović, Jelena
AU - Stevanović, Sanja
AU - Nikolić, Nebojša
AU - Petrović, Slobodan
AU - Vuković, Dragan
AU - Prlainović, Nevena
AU - Mijin, Dušan
AU - Avramov-Ivić, Milka
PY - 2017
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3570
AB - The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
PB - Electrochemical Science Group, Beograd
T2 - International Journal of Electrochemical Science
T1 - Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode
EP - 5817
IS - 7
SP - 5806
VL - 12
DO - 10.20964/2017.07.65
ER -
@article{
author = "Lović, Jelena and Stevanović, Sanja and Nikolić, Nebojša and Petrović, Slobodan and Vuković, Dragan and Prlainović, Nevena and Mijin, Dušan and Avramov-Ivić, Milka",
year = "2017",
abstract = "The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.",
publisher = "Electrochemical Science Group, Beograd",
journal = "International Journal of Electrochemical Science",
title = "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode",
pages = "5817-5806",
number = "7",
volume = "12",
doi = "10.20964/2017.07.65"
}
Lović, J., Stevanović, S., Nikolić, N., Petrović, S., Vuković, D., Prlainović, N., Mijin, D.,& Avramov-Ivić, M.. (2017). Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science
Electrochemical Science Group, Beograd., 12(7), 5806-5817.
https://doi.org/10.20964/2017.07.65
Lović J, Stevanović S, Nikolić N, Petrović S, Vuković D, Prlainović N, Mijin D, Avramov-Ivić M. Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science. 2017;12(7):5806-5817.
doi:10.20964/2017.07.65 .
Lović, Jelena, Stevanović, Sanja, Nikolić, Nebojša, Petrović, Slobodan, Vuković, Dragan, Prlainović, Nevena, Mijin, Dušan, Avramov-Ivić, Milka, "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode" in International Journal of Electrochemical Science, 12, no. 7 (2017):5806-5817,
https://doi.org/10.20964/2017.07.65 . .
