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Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application

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2017
3600.pdf (2.914Mb)
Authors
Mihajlovski, Katarina
Davidović, Slađana
Veljović, Đorđe
Simović, Milica
Lazić, Vesna M.
Dimitrijević-Branković, Suzana
Article (Published version)
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Abstract
The agricultural raw industry generates large amounts of annually by-products that create disposal problems. Hitherto, there have been no reported papers about the simultaneous production of cellulase and beta-amylase from these raw materials using Paenibacillus sp. that would reduce the costs. Thus, in this paper simultaneous cellulase (CMC-ase and avicelase) and beta-amylase production using barley bran and the application of the natural isolate Paenibacillus chitinolyticus CKS1 and potential enzymes in the hydrolysis process was studied. Response surface methodology was used to obtain the maximum enzyme activity (CMC-ase 0.405 U mL(-1), avicelase 0.433 U mL(-1) and beta-amylase 1.594U mL(-1)). Scanning electron microscopy showed degradation of the lignocellulosic-starch structure of barley bran after fermentation. The CKS1 bacterial supernatant, which contains cellulases and beta-amylase, could hydrolyze cotton fibres and barley bran, respectively. The main products after enzymatic ...hydrolysis of cotton fibres and barley bran, glucose (0.117 (-1)(g gmat)) and maltose (0.347 (-1)(g gmat)), were quantified by high performance liquid chromatography (HPLC). The produced enzymes could be used for hydrolysis of cotton fabric and barley bran to glucose and maltose, respectively. Application of simultaneous enzymes production using an agricultural by-product is economically and environmentally accepted and moreover, valuable biotechnological products, such as glucose and maltose, were obtained in this investigation.

Keywords:
agricultural by-product / P. chitinolyticus CKS1 / enzymes / process optimization / degradation / hydrolysis products
Source:
Journal of the Serbian Chemical Society, 2017, 82, 11, 1223-1236
Publisher:
  • Srpsko hemijsko društvo, Beograd
Funding / projects:
  • Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-31035)
  • Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion (RS-45020)

DOI: 10.2298/JSC170514092M

ISSN: 0352-5139

WoS: 000417845500002

Scopus: 2-s2.0-85037647294
[ Google Scholar ]
4
4
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3603
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Davidović, Slađana
AU  - Veljović, Đorđe
AU  - Simović, Milica
AU  - Lazić, Vesna M.
AU  - Dimitrijević-Branković, Suzana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3603
AB  - The agricultural raw industry generates large amounts of annually by-products that create disposal problems. Hitherto, there have been no reported papers about the simultaneous production of cellulase and beta-amylase from these raw materials using Paenibacillus sp. that would reduce the costs. Thus, in this paper simultaneous cellulase (CMC-ase and avicelase) and beta-amylase production using barley bran and the application of the natural isolate Paenibacillus chitinolyticus CKS1 and potential enzymes in the hydrolysis process was studied. Response surface methodology was used to obtain the maximum enzyme activity (CMC-ase 0.405 U mL(-1), avicelase 0.433 U mL(-1) and beta-amylase 1.594U mL(-1)). Scanning electron microscopy showed degradation of the lignocellulosic-starch structure of barley bran after fermentation. The CKS1 bacterial supernatant, which contains cellulases and beta-amylase, could hydrolyze cotton fibres and barley bran, respectively. The main products after enzymatic hydrolysis of cotton fibres and barley bran, glucose (0.117 (-1)(g gmat)) and maltose (0.347 (-1)(g gmat)), were quantified by high performance liquid chromatography (HPLC). The produced enzymes could be used for hydrolysis of cotton fabric and barley bran to glucose and maltose, respectively. Application of simultaneous enzymes production using an agricultural by-product is economically and environmentally accepted and moreover, valuable biotechnological products, such as glucose and maltose, were obtained in this investigation.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application
EP  - 1236
IS  - 11
SP  - 1223
VL  - 82
DO  - 10.2298/JSC170514092M
ER  - 
@article{
author = "Mihajlovski, Katarina and Davidović, Slađana and Veljović, Đorđe and Simović, Milica and Lazić, Vesna M. and Dimitrijević-Branković, Suzana",
year = "2017",
abstract = "The agricultural raw industry generates large amounts of annually by-products that create disposal problems. Hitherto, there have been no reported papers about the simultaneous production of cellulase and beta-amylase from these raw materials using Paenibacillus sp. that would reduce the costs. Thus, in this paper simultaneous cellulase (CMC-ase and avicelase) and beta-amylase production using barley bran and the application of the natural isolate Paenibacillus chitinolyticus CKS1 and potential enzymes in the hydrolysis process was studied. Response surface methodology was used to obtain the maximum enzyme activity (CMC-ase 0.405 U mL(-1), avicelase 0.433 U mL(-1) and beta-amylase 1.594U mL(-1)). Scanning electron microscopy showed degradation of the lignocellulosic-starch structure of barley bran after fermentation. The CKS1 bacterial supernatant, which contains cellulases and beta-amylase, could hydrolyze cotton fibres and barley bran, respectively. The main products after enzymatic hydrolysis of cotton fibres and barley bran, glucose (0.117 (-1)(g gmat)) and maltose (0.347 (-1)(g gmat)), were quantified by high performance liquid chromatography (HPLC). The produced enzymes could be used for hydrolysis of cotton fabric and barley bran to glucose and maltose, respectively. Application of simultaneous enzymes production using an agricultural by-product is economically and environmentally accepted and moreover, valuable biotechnological products, such as glucose and maltose, were obtained in this investigation.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application",
pages = "1236-1223",
number = "11",
volume = "82",
doi = "10.2298/JSC170514092M"
}
Mihajlovski, K., Davidović, S., Veljović, Đ., Simović, M., Lazić, V. M.,& Dimitrijević-Branković, S.. (2017). Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 82(11), 1223-1236.
https://doi.org/10.2298/JSC170514092M
Mihajlovski K, Davidović S, Veljović Đ, Simović M, Lazić VM, Dimitrijević-Branković S. Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application. in Journal of the Serbian Chemical Society. 2017;82(11):1223-1236.
doi:10.2298/JSC170514092M .
Mihajlovski, Katarina, Davidović, Slađana, Veljović, Đorđe, Simović, Milica, Lazić, Vesna M., Dimitrijević-Branković, Suzana, "Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application" in Journal of the Serbian Chemical Society, 82, no. 11 (2017):1223-1236,
https://doi.org/10.2298/JSC170514092M . .

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