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dc.creatorMiljković, Miona
dc.creatorDavidović, Slađana
dc.creatorKralj, Slavko
dc.creatorŠiler-Marinković, Slavica
dc.creatorRajilić-Stojanović, Mirjana
dc.creatorDimitrijević-Branković, Suzana
dc.date.accessioned2021-03-10T13:26:57Z
dc.date.available2021-03-10T13:26:57Z
dc.date.issued2017
dc.identifier.issn0367-598X
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3632
dc.description.abstractThe production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 degrees C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K2HPO4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 degrees C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca2+, Mg2+, Fe2+ and Co2+ led to a minor increase of DS activity, while the addition of Mn2+ was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries.en
dc.publisherSavez hemijskih inženjera, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceHemijska industrija
dc.subjectlactic acid bacteriaen
dc.subjectLeuconostoc mesenteroidesen
dc.subjectdextransucraseen
dc.subjectdextranen
dc.titleCharacterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolateen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage360
dc.citation.issue4
dc.citation.other71(4): 351-360
dc.citation.rankM23
dc.citation.spage351
dc.citation.volume71
dc.identifier.doi10.2298/HEMIND160421046M
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/9600/0367-598X1600046M.pdf
dc.identifier.scopus2-s2.0-85030211936
dc.identifier.wos000413180100009
dc.type.versionpublishedVersion


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Приказ основних података о документу