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TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability

Authorized Users Only
2017
Authors
Nikolić, Tanja
Korica, Matea
Milanović, Jovana
Kramar, Ana
Petronijević, Živomir
Kostić, Mirjana
Article (Published version)
Metadata
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Abstract
Cotton fibers containing different amounts of aldehyde and carboxyl groups were prepared by varying TEMPO oxidation conditions and then employed as carriers for trypsin immobilization. Depending on the functional group type present in the oxidized fiber, trypsin was immobilized through the ionic interaction or together with the Schiff's base formation. The TEMPO oxidation caused changes in the chemical and physical properties of the modified cotton, which were evaluated by determining the aldehyde and carboxyl group content, sorption and electrokinetic properties, and surface morphology of fibers. The trypsin activity was assayed with N-alpha-benzoyl- DL-arginine p-nitroanilide hydrochloride, and the protein content was determined by the Bradford method. The impact of oxidized fiber functional groups on the amount, activity and stability of the immobilized trypsin was investigated. The cotton fibers oxidized under the most severe conditions (4.84 mmol/g NaClO, 180 min) and having the h...ighest functional groups content (0.79 mmol/g) exhibited the maximum amount of immobilized trypsin of about 6.5 mg/g. However, the immobilized activity and the stability were much higher for the fibers containing both carboxyl and aldehyde groups compared to the corresponding fibers containing only carboxyl groups.

Keywords:
Cotton / TEMPO oxidation / Water retention / Zeta potential / Trypsin immobilization / Storage stability
Source:
Cellulose, 2017, 24, 4, 1863-1875
Publisher:
  • Springer, Dordrecht
Funding / projects:
  • Functionalization, characterization and application of cellulose and cellulose derivatives (RS-172029)

DOI: 10.1007/s10570-017-1221-1

ISSN: 0969-0239

WoS: 000396789100021

Scopus: 2-s2.0-85013484857
[ Google Scholar ]
25
14
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3689
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
  • Radovi istraživača (Inovacioni centar) / Researchers’ publications (Innovation Centre)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Nikolić, Tanja
AU  - Korica, Matea
AU  - Milanović, Jovana
AU  - Kramar, Ana
AU  - Petronijević, Živomir
AU  - Kostić, Mirjana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3689
AB  - Cotton fibers containing different amounts of aldehyde and carboxyl groups were prepared by varying TEMPO oxidation conditions and then employed as carriers for trypsin immobilization. Depending on the functional group type present in the oxidized fiber, trypsin was immobilized through the ionic interaction or together with the Schiff's base formation. The TEMPO oxidation caused changes in the chemical and physical properties of the modified cotton, which were evaluated by determining the aldehyde and carboxyl group content, sorption and electrokinetic properties, and surface morphology of fibers. The trypsin activity was assayed with N-alpha-benzoyl- DL-arginine p-nitroanilide hydrochloride, and the protein content was determined by the Bradford method. The impact of oxidized fiber functional groups on the amount, activity and stability of the immobilized trypsin was investigated. The cotton fibers oxidized under the most severe conditions (4.84 mmol/g NaClO, 180 min) and having the highest functional groups content (0.79 mmol/g) exhibited the maximum amount of immobilized trypsin of about 6.5 mg/g. However, the immobilized activity and the stability were much higher for the fibers containing both carboxyl and aldehyde groups compared to the corresponding fibers containing only carboxyl groups.
PB  - Springer, Dordrecht
T2  - Cellulose
T1  - TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability
EP  - 1875
IS  - 4
SP  - 1863
VL  - 24
DO  - 10.1007/s10570-017-1221-1
ER  - 
@article{
author = "Nikolić, Tanja and Korica, Matea and Milanović, Jovana and Kramar, Ana and Petronijević, Živomir and Kostić, Mirjana",
year = "2017",
abstract = "Cotton fibers containing different amounts of aldehyde and carboxyl groups were prepared by varying TEMPO oxidation conditions and then employed as carriers for trypsin immobilization. Depending on the functional group type present in the oxidized fiber, trypsin was immobilized through the ionic interaction or together with the Schiff's base formation. The TEMPO oxidation caused changes in the chemical and physical properties of the modified cotton, which were evaluated by determining the aldehyde and carboxyl group content, sorption and electrokinetic properties, and surface morphology of fibers. The trypsin activity was assayed with N-alpha-benzoyl- DL-arginine p-nitroanilide hydrochloride, and the protein content was determined by the Bradford method. The impact of oxidized fiber functional groups on the amount, activity and stability of the immobilized trypsin was investigated. The cotton fibers oxidized under the most severe conditions (4.84 mmol/g NaClO, 180 min) and having the highest functional groups content (0.79 mmol/g) exhibited the maximum amount of immobilized trypsin of about 6.5 mg/g. However, the immobilized activity and the stability were much higher for the fibers containing both carboxyl and aldehyde groups compared to the corresponding fibers containing only carboxyl groups.",
publisher = "Springer, Dordrecht",
journal = "Cellulose",
title = "TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability",
pages = "1875-1863",
number = "4",
volume = "24",
doi = "10.1007/s10570-017-1221-1"
}
Nikolić, T., Korica, M., Milanović, J., Kramar, A., Petronijević, Ž.,& Kostić, M.. (2017). TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability. in Cellulose
Springer, Dordrecht., 24(4), 1863-1875.
https://doi.org/10.1007/s10570-017-1221-1
Nikolić T, Korica M, Milanović J, Kramar A, Petronijević Ž, Kostić M. TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability. in Cellulose. 2017;24(4):1863-1875.
doi:10.1007/s10570-017-1221-1 .
Nikolić, Tanja, Korica, Matea, Milanović, Jovana, Kramar, Ana, Petronijević, Živomir, Kostić, Mirjana, "TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability" in Cellulose, 24, no. 4 (2017):1863-1875,
https://doi.org/10.1007/s10570-017-1221-1 . .

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