TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability
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2017
Authors
Nikolić, TanjaKorica, Matea

Milanović, Jovana

Kramar, Ana

Petronijević, Živomir
Kostić, Mirjana

Article (Published version)

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Cotton fibers containing different amounts of aldehyde and carboxyl groups were prepared by varying TEMPO oxidation conditions and then employed as carriers for trypsin immobilization. Depending on the functional group type present in the oxidized fiber, trypsin was immobilized through the ionic interaction or together with the Schiff's base formation. The TEMPO oxidation caused changes in the chemical and physical properties of the modified cotton, which were evaluated by determining the aldehyde and carboxyl group content, sorption and electrokinetic properties, and surface morphology of fibers. The trypsin activity was assayed with N-alpha-benzoyl- DL-arginine p-nitroanilide hydrochloride, and the protein content was determined by the Bradford method. The impact of oxidized fiber functional groups on the amount, activity and stability of the immobilized trypsin was investigated. The cotton fibers oxidized under the most severe conditions (4.84 mmol/g NaClO, 180 min) and having the h...ighest functional groups content (0.79 mmol/g) exhibited the maximum amount of immobilized trypsin of about 6.5 mg/g. However, the immobilized activity and the stability were much higher for the fibers containing both carboxyl and aldehyde groups compared to the corresponding fibers containing only carboxyl groups.
Keywords:
Cotton / TEMPO oxidation / Water retention / Zeta potential / Trypsin immobilization / Storage stabilitySource:
Cellulose, 2017, 24, 4, 1863-1875Publisher:
- Springer, Dordrecht
Funding / projects:
- Functionalization, characterization and application of cellulose and cellulose derivatives (RS-172029)
DOI: 10.1007/s10570-017-1221-1
ISSN: 0969-0239
WoS: 000396789100021
Scopus: 2-s2.0-85013484857
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Tehnološko-metalurški fakultetTY - JOUR AU - Nikolić, Tanja AU - Korica, Matea AU - Milanović, Jovana AU - Kramar, Ana AU - Petronijević, Živomir AU - Kostić, Mirjana PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3689 AB - Cotton fibers containing different amounts of aldehyde and carboxyl groups were prepared by varying TEMPO oxidation conditions and then employed as carriers for trypsin immobilization. Depending on the functional group type present in the oxidized fiber, trypsin was immobilized through the ionic interaction or together with the Schiff's base formation. The TEMPO oxidation caused changes in the chemical and physical properties of the modified cotton, which were evaluated by determining the aldehyde and carboxyl group content, sorption and electrokinetic properties, and surface morphology of fibers. The trypsin activity was assayed with N-alpha-benzoyl- DL-arginine p-nitroanilide hydrochloride, and the protein content was determined by the Bradford method. The impact of oxidized fiber functional groups on the amount, activity and stability of the immobilized trypsin was investigated. The cotton fibers oxidized under the most severe conditions (4.84 mmol/g NaClO, 180 min) and having the highest functional groups content (0.79 mmol/g) exhibited the maximum amount of immobilized trypsin of about 6.5 mg/g. However, the immobilized activity and the stability were much higher for the fibers containing both carboxyl and aldehyde groups compared to the corresponding fibers containing only carboxyl groups. PB - Springer, Dordrecht T2 - Cellulose T1 - TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability EP - 1875 IS - 4 SP - 1863 VL - 24 DO - 10.1007/s10570-017-1221-1 ER -
@article{ author = "Nikolić, Tanja and Korica, Matea and Milanović, Jovana and Kramar, Ana and Petronijević, Živomir and Kostić, Mirjana", year = "2017", abstract = "Cotton fibers containing different amounts of aldehyde and carboxyl groups were prepared by varying TEMPO oxidation conditions and then employed as carriers for trypsin immobilization. Depending on the functional group type present in the oxidized fiber, trypsin was immobilized through the ionic interaction or together with the Schiff's base formation. The TEMPO oxidation caused changes in the chemical and physical properties of the modified cotton, which were evaluated by determining the aldehyde and carboxyl group content, sorption and electrokinetic properties, and surface morphology of fibers. The trypsin activity was assayed with N-alpha-benzoyl- DL-arginine p-nitroanilide hydrochloride, and the protein content was determined by the Bradford method. The impact of oxidized fiber functional groups on the amount, activity and stability of the immobilized trypsin was investigated. The cotton fibers oxidized under the most severe conditions (4.84 mmol/g NaClO, 180 min) and having the highest functional groups content (0.79 mmol/g) exhibited the maximum amount of immobilized trypsin of about 6.5 mg/g. However, the immobilized activity and the stability were much higher for the fibers containing both carboxyl and aldehyde groups compared to the corresponding fibers containing only carboxyl groups.", publisher = "Springer, Dordrecht", journal = "Cellulose", title = "TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability", pages = "1875-1863", number = "4", volume = "24", doi = "10.1007/s10570-017-1221-1" }
Nikolić, T., Korica, M., Milanović, J., Kramar, A., Petronijević, Ž.,& Kostić, M.. (2017). TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability. in Cellulose Springer, Dordrecht., 24(4), 1863-1875. https://doi.org/10.1007/s10570-017-1221-1
Nikolić T, Korica M, Milanović J, Kramar A, Petronijević Ž, Kostić M. TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability. in Cellulose. 2017;24(4):1863-1875. doi:10.1007/s10570-017-1221-1 .
Nikolić, Tanja, Korica, Matea, Milanović, Jovana, Kramar, Ana, Petronijević, Živomir, Kostić, Mirjana, "TEMPO-oxidized cotton as a substrate for trypsin immobilization: impact of functional groups on proteolytic activity and stability" in Cellulose, 24, no. 4 (2017):1863-1875, https://doi.org/10.1007/s10570-017-1221-1 . .