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Integration of engineered cartilage

Authorized Users Only
2001
Authors
Obradović, Bojana
Martin, Ivan
Padera, RF
Treppo, S
Freed, LE
Vunjak-Novaković, Gordana
Article (Published version)
Metadata
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Abstract
The structure and function of cartilaginous constructs, engineered in vitro using bovine articular chondrocytes, biodegradable scaffolds and bioreactors, can be modulated by the conditions and duration of tissue cultivation. We hypothesized that the integrative properties of engineered cartilage depend on developmental stage of the construct and the extracellular matrix content of adjacent cartilage, and that some aspects of integration can be studied under controlled in vitro conditions. Disc-shaped constructs (cultured for 5 +/- 1 days or 5 +/- 1 weeks) or explants (untreated or trypsin treated cartilage) were sutured into ring-shaped explants (untreated or trypsin treated cartilage) to form composites that were cultured for an additional 1-8 weeks in bioreactors and evaluated biochemically, histologically and mechanically (compressive stiffness of the central disk, adhesive strength of the integration interface). Immature constructs had poorer mechanical properties but integrated be...tter than either more mature constructs or cartilage explants. Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components. Integration patterns correlated with the adhesive strength of the disc-ring interface, which was markedly higher for immature constructs than for either more mature constructs or cartilage explants. Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs.

Source:
Journal of Orthopaedic Research, 2001, 19, 6, 1089-1097
Publisher:
  • Elsevier Sci Ltd, Oxford

DOI: 10.1016/S0736-0266(01)00030-4

ISSN: 0736-0266

PubMed: 11781010

WoS: 000172916000015

Scopus: 2-s2.0-0035215793
[ Google Scholar ]
207
191
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/371
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Obradović, Bojana
AU  - Martin, Ivan
AU  - Padera, RF
AU  - Treppo, S
AU  - Freed, LE
AU  - Vunjak-Novaković, Gordana
PY  - 2001
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/371
AB  - The structure and function of cartilaginous constructs, engineered in vitro using bovine articular chondrocytes, biodegradable scaffolds and bioreactors, can be modulated by the conditions and duration of tissue cultivation. We hypothesized that the integrative properties of engineered cartilage depend on developmental stage of the construct and the extracellular matrix content of adjacent cartilage, and that some aspects of integration can be studied under controlled in vitro conditions. Disc-shaped constructs (cultured for 5 +/- 1 days or 5 +/- 1 weeks) or explants (untreated or trypsin treated cartilage) were sutured into ring-shaped explants (untreated or trypsin treated cartilage) to form composites that were cultured for an additional 1-8 weeks in bioreactors and evaluated biochemically, histologically and mechanically (compressive stiffness of the central disk, adhesive strength of the integration interface). Immature constructs had poorer mechanical properties but integrated better than either more mature constructs or cartilage explants. Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components. Integration patterns correlated with the adhesive strength of the disc-ring interface, which was markedly higher for immature constructs than for either more mature constructs or cartilage explants. Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Orthopaedic Research
T1  - Integration of engineered cartilage
EP  - 1097
IS  - 6
SP  - 1089
VL  - 19
DO  - 10.1016/S0736-0266(01)00030-4
ER  - 
@article{
author = "Obradović, Bojana and Martin, Ivan and Padera, RF and Treppo, S and Freed, LE and Vunjak-Novaković, Gordana",
year = "2001",
abstract = "The structure and function of cartilaginous constructs, engineered in vitro using bovine articular chondrocytes, biodegradable scaffolds and bioreactors, can be modulated by the conditions and duration of tissue cultivation. We hypothesized that the integrative properties of engineered cartilage depend on developmental stage of the construct and the extracellular matrix content of adjacent cartilage, and that some aspects of integration can be studied under controlled in vitro conditions. Disc-shaped constructs (cultured for 5 +/- 1 days or 5 +/- 1 weeks) or explants (untreated or trypsin treated cartilage) were sutured into ring-shaped explants (untreated or trypsin treated cartilage) to form composites that were cultured for an additional 1-8 weeks in bioreactors and evaluated biochemically, histologically and mechanically (compressive stiffness of the central disk, adhesive strength of the integration interface). Immature constructs had poorer mechanical properties but integrated better than either more mature constructs or cartilage explants. Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components. Integration patterns correlated with the adhesive strength of the disc-ring interface, which was markedly higher for immature constructs than for either more mature constructs or cartilage explants. Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Orthopaedic Research",
title = "Integration of engineered cartilage",
pages = "1097-1089",
number = "6",
volume = "19",
doi = "10.1016/S0736-0266(01)00030-4"
}
Obradović, B., Martin, I., Padera, R., Treppo, S., Freed, L.,& Vunjak-Novaković, G.. (2001). Integration of engineered cartilage. in Journal of Orthopaedic Research
Elsevier Sci Ltd, Oxford., 19(6), 1089-1097.
https://doi.org/10.1016/S0736-0266(01)00030-4
Obradović B, Martin I, Padera R, Treppo S, Freed L, Vunjak-Novaković G. Integration of engineered cartilage. in Journal of Orthopaedic Research. 2001;19(6):1089-1097.
doi:10.1016/S0736-0266(01)00030-4 .
Obradović, Bojana, Martin, Ivan, Padera, RF, Treppo, S, Freed, LE, Vunjak-Novaković, Gordana, "Integration of engineered cartilage" in Journal of Orthopaedic Research, 19, no. 6 (2001):1089-1097,
https://doi.org/10.1016/S0736-0266(01)00030-4 . .

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