Integration of engineered cartilage
Само за регистроване кориснике
2001
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The structure and function of cartilaginous constructs, engineered in vitro using bovine articular chondrocytes, biodegradable scaffolds and bioreactors, can be modulated by the conditions and duration of tissue cultivation. We hypothesized that the integrative properties of engineered cartilage depend on developmental stage of the construct and the extracellular matrix content of adjacent cartilage, and that some aspects of integration can be studied under controlled in vitro conditions. Disc-shaped constructs (cultured for 5 +/- 1 days or 5 +/- 1 weeks) or explants (untreated or trypsin treated cartilage) were sutured into ring-shaped explants (untreated or trypsin treated cartilage) to form composites that were cultured for an additional 1-8 weeks in bioreactors and evaluated biochemically, histologically and mechanically (compressive stiffness of the central disk, adhesive strength of the integration interface). Immature constructs had poorer mechanical properties but integrated be...tter than either more mature constructs or cartilage explants. Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components. Integration patterns correlated with the adhesive strength of the disc-ring interface, which was markedly higher for immature constructs than for either more mature constructs or cartilage explants. Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs.
Извор:
Journal of Orthopaedic Research, 2001, 19, 6, 1089-1097Издавач:
- Elsevier Sci Ltd, Oxford
DOI: 10.1016/S0736-0266(01)00030-4
ISSN: 0736-0266
PubMed: 11781010
WoS: 000172916000015
Scopus: 2-s2.0-0035215793
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Obradović, Bojana AU - Martin, Ivan AU - Padera, RF AU - Treppo, S AU - Freed, LE AU - Vunjak-Novaković, Gordana PY - 2001 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/371 AB - The structure and function of cartilaginous constructs, engineered in vitro using bovine articular chondrocytes, biodegradable scaffolds and bioreactors, can be modulated by the conditions and duration of tissue cultivation. We hypothesized that the integrative properties of engineered cartilage depend on developmental stage of the construct and the extracellular matrix content of adjacent cartilage, and that some aspects of integration can be studied under controlled in vitro conditions. Disc-shaped constructs (cultured for 5 +/- 1 days or 5 +/- 1 weeks) or explants (untreated or trypsin treated cartilage) were sutured into ring-shaped explants (untreated or trypsin treated cartilage) to form composites that were cultured for an additional 1-8 weeks in bioreactors and evaluated biochemically, histologically and mechanically (compressive stiffness of the central disk, adhesive strength of the integration interface). Immature constructs had poorer mechanical properties but integrated better than either more mature constructs or cartilage explants. Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components. Integration patterns correlated with the adhesive strength of the disc-ring interface, which was markedly higher for immature constructs than for either more mature constructs or cartilage explants. Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs. PB - Elsevier Sci Ltd, Oxford T2 - Journal of Orthopaedic Research T1 - Integration of engineered cartilage EP - 1097 IS - 6 SP - 1089 VL - 19 DO - 10.1016/S0736-0266(01)00030-4 ER -
@article{ author = "Obradović, Bojana and Martin, Ivan and Padera, RF and Treppo, S and Freed, LE and Vunjak-Novaković, Gordana", year = "2001", abstract = "The structure and function of cartilaginous constructs, engineered in vitro using bovine articular chondrocytes, biodegradable scaffolds and bioreactors, can be modulated by the conditions and duration of tissue cultivation. We hypothesized that the integrative properties of engineered cartilage depend on developmental stage of the construct and the extracellular matrix content of adjacent cartilage, and that some aspects of integration can be studied under controlled in vitro conditions. Disc-shaped constructs (cultured for 5 +/- 1 days or 5 +/- 1 weeks) or explants (untreated or trypsin treated cartilage) were sutured into ring-shaped explants (untreated or trypsin treated cartilage) to form composites that were cultured for an additional 1-8 weeks in bioreactors and evaluated biochemically, histologically and mechanically (compressive stiffness of the central disk, adhesive strength of the integration interface). Immature constructs had poorer mechanical properties but integrated better than either more mature constructs or cartilage explants. Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components. Integration patterns correlated with the adhesive strength of the disc-ring interface, which was markedly higher for immature constructs than for either more mature constructs or cartilage explants. Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Journal of Orthopaedic Research", title = "Integration of engineered cartilage", pages = "1097-1089", number = "6", volume = "19", doi = "10.1016/S0736-0266(01)00030-4" }
Obradović, B., Martin, I., Padera, R., Treppo, S., Freed, L.,& Vunjak-Novaković, G.. (2001). Integration of engineered cartilage. in Journal of Orthopaedic Research Elsevier Sci Ltd, Oxford., 19(6), 1089-1097. https://doi.org/10.1016/S0736-0266(01)00030-4
Obradović B, Martin I, Padera R, Treppo S, Freed L, Vunjak-Novaković G. Integration of engineered cartilage. in Journal of Orthopaedic Research. 2001;19(6):1089-1097. doi:10.1016/S0736-0266(01)00030-4 .
Obradović, Bojana, Martin, Ivan, Padera, RF, Treppo, S, Freed, LE, Vunjak-Novaković, Gordana, "Integration of engineered cartilage" in Journal of Orthopaedic Research, 19, no. 6 (2001):1089-1097, https://doi.org/10.1016/S0736-0266(01)00030-4 . .