Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study
Само за регистроване кориснике
2017
Аутори
Dojčilović, RadovanPajović, Jelena D.
Božanić, Dušan K.
Bogdanović, Una
Vodnik, Vesna
Dimitrijević-Branković, Suzana
Miljković, Miona
Kascakova, Slavka
Refregiers, Matthieu
Đoković, Vladimir
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. Th...e analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells' surfaces.
Кључне речи:
Silver nanoparticles / Tryptophan / Functionalization / C. albicans / Fluorescence imagingИзвор:
Colloids and Surfaces B-Biointerfaces, 2017, 155, 341-348Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- [20131218]
- Утицај величине, облика и структуре наночестица на њихова својства и својства нанокомпозита (RS-172056)
- Материјали редуковане димензионалности за ефикасну апсорпцију светлости и конверзију енергије (RS-45020)
- Проучавање утицаја третирања на диелектричне, оптичке, магнетне и особине површина кристалних и полимерних система (RS-171029)
DOI: 10.1016/j.colsurfb.2017.04.044
ISSN: 0927-7765
PubMed: 28454063
WoS: 000403738000040
Scopus: 2-s2.0-85018651384
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Dojčilović, Radovan AU - Pajović, Jelena D. AU - Božanić, Dušan K. AU - Bogdanović, Una AU - Vodnik, Vesna AU - Dimitrijević-Branković, Suzana AU - Miljković, Miona AU - Kascakova, Slavka AU - Refregiers, Matthieu AU - Đoković, Vladimir PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3726 AB - The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells' surfaces. PB - Elsevier Science Bv, Amsterdam T2 - Colloids and Surfaces B-Biointerfaces T1 - Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study EP - 348 SP - 341 VL - 155 DO - 10.1016/j.colsurfb.2017.04.044 ER -
@article{ author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Bogdanović, Una and Vodnik, Vesna and Dimitrijević-Branković, Suzana and Miljković, Miona and Kascakova, Slavka and Refregiers, Matthieu and Đoković, Vladimir", year = "2017", abstract = "The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells' surfaces.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Colloids and Surfaces B-Biointerfaces", title = "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study", pages = "348-341", volume = "155", doi = "10.1016/j.colsurfb.2017.04.044" }
Dojčilović, R., Pajović, J. D., Božanić, D. K., Bogdanović, U., Vodnik, V., Dimitrijević-Branković, S., Miljković, M., Kascakova, S., Refregiers, M.,& Đoković, V.. (2017). Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces B-Biointerfaces Elsevier Science Bv, Amsterdam., 155, 341-348. https://doi.org/10.1016/j.colsurfb.2017.04.044
Dojčilović R, Pajović JD, Božanić DK, Bogdanović U, Vodnik V, Dimitrijević-Branković S, Miljković M, Kascakova S, Refregiers M, Đoković V. Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces B-Biointerfaces. 2017;155:341-348. doi:10.1016/j.colsurfb.2017.04.044 .
Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Bogdanović, Una, Vodnik, Vesna, Dimitrijević-Branković, Suzana, Miljković, Miona, Kascakova, Slavka, Refregiers, Matthieu, Đoković, Vladimir, "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study" in Colloids and Surfaces B-Biointerfaces, 155 (2017):341-348, https://doi.org/10.1016/j.colsurfb.2017.04.044 . .