Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells

2018
Authors
Trivanović, Drenka
Drvenica, Ivana

Kukolj, Tamara
Obradović, Hristina
Okić-Đorđević, Ivana

Mojsilović, Slavko

Krstić, Jelena

Bugarski, Branko

Jauković, Aleksandra

Bugarski, Diana
Article (Published version)
Metadata
Show full item recordAbstract
Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was f...ollowed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.
Keywords:
Adipose stem cells / extracellular matrix / differentiation / cytoskeleton / mitochondria / SIRT1Source:
Artificial Cells Nanomedicine and Biotechnology, 2018, 46, S370-S382Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Regenerative and modulatory potential of adult stem cells (RS-175062)
- fellowship "Start up for Science, Serbia" 2015/2016 (Company Philip Morris)
DOI: 10.1080/21691401.2018.1494183
ISSN: 2169-1401
PubMed: 30198336
WoS: 000460141900036
Scopus: 2-s2.0-85053249346
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Trivanović, Drenka AU - Drvenica, Ivana AU - Kukolj, Tamara AU - Obradović, Hristina AU - Okić-Đorđević, Ivana AU - Mojsilović, Slavko AU - Krstić, Jelena AU - Bugarski, Branko AU - Jauković, Aleksandra AU - Bugarski, Diana PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3761 AB - Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT. PB - Taylor & Francis Ltd, Abingdon T2 - Artificial Cells Nanomedicine and Biotechnology T1 - Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells EP - S382 SP - S370 VL - 46 DO - 10.1080/21691401.2018.1494183 ER -
@article{ author = "Trivanović, Drenka and Drvenica, Ivana and Kukolj, Tamara and Obradović, Hristina and Okić-Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Bugarski, Branko and Jauković, Aleksandra and Bugarski, Diana", year = "2018", abstract = "Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Artificial Cells Nanomedicine and Biotechnology", title = "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells", pages = "S382-S370", volume = "46", doi = "10.1080/21691401.2018.1494183" }
Trivanović, D., Drvenica, I., Kukolj, T., Obradović, H., Okić-Đorđević, I., Mojsilović, S., Krstić, J., Bugarski, B., Jauković, A.,& Bugarski, D.. (2018). Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine and Biotechnology Taylor & Francis Ltd, Abingdon., 46, S370-S382. https://doi.org/10.1080/21691401.2018.1494183
Trivanović D, Drvenica I, Kukolj T, Obradović H, Okić-Đorđević I, Mojsilović S, Krstić J, Bugarski B, Jauković A, Bugarski D. Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine and Biotechnology. 2018;46:S370-S382. doi:10.1080/21691401.2018.1494183 .
Trivanović, Drenka, Drvenica, Ivana, Kukolj, Tamara, Obradović, Hristina, Okić-Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Bugarski, Branko, Jauković, Aleksandra, Bugarski, Diana, "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells" in Artificial Cells Nanomedicine and Biotechnology, 46 (2018):S370-S382, https://doi.org/10.1080/21691401.2018.1494183 . .