Wheat straw is the major crop residue in Europe which makes it the most promising material for bioconversion. However, only 3% of annual production is used directly while the rest is considered as waste. A key step in processing of lignocellulosics is delignification by fungi and the presence of calcium can be of a great importance as it can modulate ligninolytic enzymes activities. Trametes gibbosa BEOFB 310 was used for solid-state fermentation of wheat straw in the presence of calcium in different concentrations. Samples were extracted after 19days of fermentation and activities of Mn-oxidizing peroxidases and laccase were determined spectrophotometrically. Quantitative procedures were used to determine contents of hemicelluloses, cellulose, and lignin. Calcium induced activity of Mn-dependent peroxidase to a concentration of 5.0mM (7185.2 +/- 791.4 UL-1), which was threefold higher than in the control. Lignocellulose loss in wheat straw was stimulated by calcium addition and the ma...ximum delignification was detected at concentration of 5.0mM (52.9 +/- 0.9%). Delignification was positively correlated to activity of Mn-dependent peroxidase. This study showed that wheat straw supplementation with calcium can significantly improve solid-state fermentation by increasing Trametes gibbosa Mn-dependent peroxidase activity and augmenting lignin degradation.
TY - JOUR
AU - Knezević, Aleksandar
AU - Stajić, Mirjana
AU - Milovanović, Ivan
AU - Vukojević, Jelena
PY - 2018
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3842
AB - Wheat straw is the major crop residue in Europe which makes it the most promising material for bioconversion. However, only 3% of annual production is used directly while the rest is considered as waste. A key step in processing of lignocellulosics is delignification by fungi and the presence of calcium can be of a great importance as it can modulate ligninolytic enzymes activities. Trametes gibbosa BEOFB 310 was used for solid-state fermentation of wheat straw in the presence of calcium in different concentrations. Samples were extracted after 19days of fermentation and activities of Mn-oxidizing peroxidases and laccase were determined spectrophotometrically. Quantitative procedures were used to determine contents of hemicelluloses, cellulose, and lignin. Calcium induced activity of Mn-dependent peroxidase to a concentration of 5.0mM (7185.2 +/- 791.4 UL-1), which was threefold higher than in the control. Lignocellulose loss in wheat straw was stimulated by calcium addition and the maximum delignification was detected at concentration of 5.0mM (52.9 +/- 0.9%). Delignification was positively correlated to activity of Mn-dependent peroxidase. This study showed that wheat straw supplementation with calcium can significantly improve solid-state fermentation by increasing Trametes gibbosa Mn-dependent peroxidase activity and augmenting lignin degradation.
PB - Springer, Dordrecht
T2 - Waste and Biomass Valorization
T1 - Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions
EP - 1908
IS - 10
SP - 1903
VL - 9
DO - 10.1007/s12649-017-9961-6
ER -
@article{
author = "Knezević, Aleksandar and Stajić, Mirjana and Milovanović, Ivan and Vukojević, Jelena",
year = "2018",
abstract = "Wheat straw is the major crop residue in Europe which makes it the most promising material for bioconversion. However, only 3% of annual production is used directly while the rest is considered as waste. A key step in processing of lignocellulosics is delignification by fungi and the presence of calcium can be of a great importance as it can modulate ligninolytic enzymes activities. Trametes gibbosa BEOFB 310 was used for solid-state fermentation of wheat straw in the presence of calcium in different concentrations. Samples were extracted after 19days of fermentation and activities of Mn-oxidizing peroxidases and laccase were determined spectrophotometrically. Quantitative procedures were used to determine contents of hemicelluloses, cellulose, and lignin. Calcium induced activity of Mn-dependent peroxidase to a concentration of 5.0mM (7185.2 +/- 791.4 UL-1), which was threefold higher than in the control. Lignocellulose loss in wheat straw was stimulated by calcium addition and the maximum delignification was detected at concentration of 5.0mM (52.9 +/- 0.9%). Delignification was positively correlated to activity of Mn-dependent peroxidase. This study showed that wheat straw supplementation with calcium can significantly improve solid-state fermentation by increasing Trametes gibbosa Mn-dependent peroxidase activity and augmenting lignin degradation.",
publisher = "Springer, Dordrecht",
journal = "Waste and Biomass Valorization",
title = "Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions",
pages = "1908-1903",
number = "10",
volume = "9",
doi = "10.1007/s12649-017-9961-6"
}
Knezević, A., Stajić, M., Milovanović, I.,& Vukojević, J.. (2018). Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions. in Waste and Biomass Valorization
Springer, Dordrecht., 9(10), 1903-1908.
https://doi.org/10.1007/s12649-017-9961-6
Knezević A, Stajić M, Milovanović I, Vukojević J. Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions. in Waste and Biomass Valorization. 2018;9(10):1903-1908.
doi:10.1007/s12649-017-9961-6 .
Knezević, Aleksandar, Stajić, Mirjana, Milovanović, Ivan, Vukojević, Jelena, "Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions" in Waste and Biomass Valorization, 9, no. 10 (2018):1903-1908,
https://doi.org/10.1007/s12649-017-9961-6 . .
