Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions
Abstract
Wheat straw is the major crop residue in Europe which makes it the most promising material for bioconversion. However, only 3% of annual production is used directly while the rest is considered as waste. A key step in processing of lignocellulosics is delignification by fungi and the presence of calcium can be of a great importance as it can modulate ligninolytic enzymes activities. Trametes gibbosa BEOFB 310 was used for solid-state fermentation of wheat straw in the presence of calcium in different concentrations. Samples were extracted after 19days of fermentation and activities of Mn-oxidizing peroxidases and laccase were determined spectrophotometrically. Quantitative procedures were used to determine contents of hemicelluloses, cellulose, and lignin. Calcium induced activity of Mn-dependent peroxidase to a concentration of 5.0mM (7185.2 +/- 791.4 UL-1), which was threefold higher than in the control. Lignocellulose loss in wheat straw was stimulated by calcium addition and the ma...ximum delignification was detected at concentration of 5.0mM (52.9 +/- 0.9%). Delignification was positively correlated to activity of Mn-dependent peroxidase. This study showed that wheat straw supplementation with calcium can significantly improve solid-state fermentation by increasing Trametes gibbosa Mn-dependent peroxidase activity and augmenting lignin degradation.
Keywords:
Calcium / Laccase / Mn-oxidizing peroxidases / Trametes gibbosa / Wheat strawSource:
Waste and Biomass Valorization, 2018, 9, 10, 1903-1908Publisher:
- Springer, Dordrecht
Funding / projects:
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
DOI: 10.1007/s12649-017-9961-6
ISSN: 1877-2641
WoS: 000444478500018
Scopus: 2-s2.0-85019067473
Institution/Community
Inovacioni centarTY - JOUR AU - Knezević, Aleksandar AU - Stajić, Mirjana AU - Milovanović, Ivan AU - Vukojević, Jelena PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3842 AB - Wheat straw is the major crop residue in Europe which makes it the most promising material for bioconversion. However, only 3% of annual production is used directly while the rest is considered as waste. A key step in processing of lignocellulosics is delignification by fungi and the presence of calcium can be of a great importance as it can modulate ligninolytic enzymes activities. Trametes gibbosa BEOFB 310 was used for solid-state fermentation of wheat straw in the presence of calcium in different concentrations. Samples were extracted after 19days of fermentation and activities of Mn-oxidizing peroxidases and laccase were determined spectrophotometrically. Quantitative procedures were used to determine contents of hemicelluloses, cellulose, and lignin. Calcium induced activity of Mn-dependent peroxidase to a concentration of 5.0mM (7185.2 +/- 791.4 UL-1), which was threefold higher than in the control. Lignocellulose loss in wheat straw was stimulated by calcium addition and the maximum delignification was detected at concentration of 5.0mM (52.9 +/- 0.9%). Delignification was positively correlated to activity of Mn-dependent peroxidase. This study showed that wheat straw supplementation with calcium can significantly improve solid-state fermentation by increasing Trametes gibbosa Mn-dependent peroxidase activity and augmenting lignin degradation. PB - Springer, Dordrecht T2 - Waste and Biomass Valorization T1 - Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions EP - 1908 IS - 10 SP - 1903 VL - 9 DO - 10.1007/s12649-017-9961-6 ER -
@article{ author = "Knezević, Aleksandar and Stajić, Mirjana and Milovanović, Ivan and Vukojević, Jelena", year = "2018", abstract = "Wheat straw is the major crop residue in Europe which makes it the most promising material for bioconversion. However, only 3% of annual production is used directly while the rest is considered as waste. A key step in processing of lignocellulosics is delignification by fungi and the presence of calcium can be of a great importance as it can modulate ligninolytic enzymes activities. Trametes gibbosa BEOFB 310 was used for solid-state fermentation of wheat straw in the presence of calcium in different concentrations. Samples were extracted after 19days of fermentation and activities of Mn-oxidizing peroxidases and laccase were determined spectrophotometrically. Quantitative procedures were used to determine contents of hemicelluloses, cellulose, and lignin. Calcium induced activity of Mn-dependent peroxidase to a concentration of 5.0mM (7185.2 +/- 791.4 UL-1), which was threefold higher than in the control. Lignocellulose loss in wheat straw was stimulated by calcium addition and the maximum delignification was detected at concentration of 5.0mM (52.9 +/- 0.9%). Delignification was positively correlated to activity of Mn-dependent peroxidase. This study showed that wheat straw supplementation with calcium can significantly improve solid-state fermentation by increasing Trametes gibbosa Mn-dependent peroxidase activity and augmenting lignin degradation.", publisher = "Springer, Dordrecht", journal = "Waste and Biomass Valorization", title = "Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions", pages = "1908-1903", number = "10", volume = "9", doi = "10.1007/s12649-017-9961-6" }
Knezević, A., Stajić, M., Milovanović, I.,& Vukojević, J.. (2018). Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions. in Waste and Biomass Valorization Springer, Dordrecht., 9(10), 1903-1908. https://doi.org/10.1007/s12649-017-9961-6
Knezević A, Stajić M, Milovanović I, Vukojević J. Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions. in Waste and Biomass Valorization. 2018;9(10):1903-1908. doi:10.1007/s12649-017-9961-6 .
Knezević, Aleksandar, Stajić, Mirjana, Milovanović, Ivan, Vukojević, Jelena, "Wheat Straw Degradation by Trametes gibbosa: The Effect of Calcium Ions" in Waste and Biomass Valorization, 9, no. 10 (2018):1903-1908, https://doi.org/10.1007/s12649-017-9961-6 . .