NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells
2018
Аутори
Mančić, LidijaĐukić-Vuković, Aleksandra
Dinić, Ivana
Nikolić, Marko G.
Rabasović, Mihailo D.
Krmpot, Aleksandar
Costa, Antonio Mario Leal Martins
Trišić, Dijana
Lazarević, Miloš
Mojović, Ljiljana
Milošević, Olivera
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P6(3)/m) phase in spherical and irregularly shaped nanoparticles, res...pectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (lambda(ex) = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10-50 mu g/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy.
Кључне речи:
Upconversion nanoparticles / PLGA / NaYF4:Yb,Er / Bioimaging / Cancer cell / Human gingival cellИзвор:
Materials Science & Engineering C-Materials for Biological Applications, 2018, 91, 597-605Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Рационални дизајн и синтеза биолошки активних и координационих једињења и функционалних материјала, релевантних у (био)нанотехнологији (RS-MESTD-Basic Research (BR or ON)-172035)
- Генерисање и карактеризација нанофотонских функционалних структура у биомедицини и информатици (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45016)
Напомена:
- Supplementary information: https://technorep.tmf.bg.ac.rs/handle/123456789/3769
Повезане информације:
DOI: 10.1016/j.msec.2018.05.081
ISSN: 0928-4931
PubMed: 30033292
WoS: 000442192000061
Scopus: 2-s2.0-85047855276
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Mančić, Lidija AU - Đukić-Vuković, Aleksandra AU - Dinić, Ivana AU - Nikolić, Marko G. AU - Rabasović, Mihailo D. AU - Krmpot, Aleksandar AU - Costa, Antonio Mario Leal Martins AU - Trišić, Dijana AU - Lazarević, Miloš AU - Mojović, Ljiljana AU - Milošević, Olivera PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3852 AB - Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P6(3)/m) phase in spherical and irregularly shaped nanoparticles, respectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (lambda(ex) = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10-50 mu g/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy. PB - Elsevier Science Bv, Amsterdam T2 - Materials Science & Engineering C-Materials for Biological Applications T1 - NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells EP - 605 SP - 597 VL - 91 DO - 10.1016/j.msec.2018.05.081 ER -
@article{ author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar and Costa, Antonio Mario Leal Martins and Trišić, Dijana and Lazarević, Miloš and Mojović, Ljiljana and Milošević, Olivera", year = "2018", abstract = "Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P6(3)/m) phase in spherical and irregularly shaped nanoparticles, respectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (lambda(ex) = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10-50 mu g/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Materials Science & Engineering C-Materials for Biological Applications", title = "NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells", pages = "605-597", volume = "91", doi = "10.1016/j.msec.2018.05.081" }
Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A., Costa, A. M. L. M., Trišić, D., Lazarević, M., Mojović, L.,& Milošević, O.. (2018). NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells. in Materials Science & Engineering C-Materials for Biological Applications Elsevier Science Bv, Amsterdam., 91, 597-605. https://doi.org/10.1016/j.msec.2018.05.081
Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot A, Costa AMLM, Trišić D, Lazarević M, Mojović L, Milošević O. NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells. in Materials Science & Engineering C-Materials for Biological Applications. 2018;91:597-605. doi:10.1016/j.msec.2018.05.081 .
Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar, Costa, Antonio Mario Leal Martins, Trišić, Dijana, Lazarević, Miloš, Mojović, Ljiljana, Milošević, Olivera, "NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells" in Materials Science & Engineering C-Materials for Biological Applications, 91 (2018):597-605, https://doi.org/10.1016/j.msec.2018.05.081 . .