Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers
Abstract
Gelatin hydrogels have great potential in regenerative medicine but their weak mechanical properties are a major drawback for the load-bearing applications, such as scaffolds for tissue engineering. To overcome this deficiency, novel biodegradable hydrogels with improved mechanical properties were prepared by combining gelatine with 2-hydroxyethyl methacrylate (HEMA), using a double network synthetic procedure. The first, superporous and mechanically strong network, was obtained by free radical polymerization of HEMA at cryogenic temperature, in the presence of gelatin. Degradable poly (beta-amino ester) (PBAE) macromers of different chemical composition or molecular weight were used as crosslinkers to introduce hydrolytically labile bonds in PHEMA. The second gelatin network was formed by crosslinking gelatin with glutaraldehyde. For comparison, a set of biodegradable PHEMA networks was obtained by polymerization of HEMA at cryogenic temperature. All samples were characterized reveali...ng that mechanical strength, swelling behavior and degradation rate as well as high biocompatibility of new IPNs are in accordance with values required for scaffolds in tissue engineering applications and that tuning of these properties is accomplished by simply using different PBAE macromers.
Keywords:
HEMA / PBAE / Biocompatible / Degradable / Porous scaffoldsSource:
Polymer Testing, 2018, 68, 270-278Publisher:
- Elsevier Sci Ltd, Oxford
Funding / projects:
- SCOPES programme of the Swiss National Science Foundation
- Swiss Agency for Development and Cooperation [IZ73ZO_152327]
- Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-172062)
- Geologic and ecotoxicologic research in identification of geopathogen zones of toxic elements in drinking water reservoirs- research into methods and procedures for reduction of biochemical anomalies (RS-176018)
DOI: 10.1016/j.polymertesting.2018.04.024
ISSN: 0142-9418
WoS: 000437076000034
Scopus: 2-s2.0-85046032696
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Filipović, Vuk AU - Božić-Nedeljković, Biljana AU - Vukomanović, Marija AU - Tomić, Simonida PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3874 AB - Gelatin hydrogels have great potential in regenerative medicine but their weak mechanical properties are a major drawback for the load-bearing applications, such as scaffolds for tissue engineering. To overcome this deficiency, novel biodegradable hydrogels with improved mechanical properties were prepared by combining gelatine with 2-hydroxyethyl methacrylate (HEMA), using a double network synthetic procedure. The first, superporous and mechanically strong network, was obtained by free radical polymerization of HEMA at cryogenic temperature, in the presence of gelatin. Degradable poly (beta-amino ester) (PBAE) macromers of different chemical composition or molecular weight were used as crosslinkers to introduce hydrolytically labile bonds in PHEMA. The second gelatin network was formed by crosslinking gelatin with glutaraldehyde. For comparison, a set of biodegradable PHEMA networks was obtained by polymerization of HEMA at cryogenic temperature. All samples were characterized revealing that mechanical strength, swelling behavior and degradation rate as well as high biocompatibility of new IPNs are in accordance with values required for scaffolds in tissue engineering applications and that tuning of these properties is accomplished by simply using different PBAE macromers. PB - Elsevier Sci Ltd, Oxford T2 - Polymer Testing T1 - Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers EP - 278 SP - 270 VL - 68 DO - 10.1016/j.polymertesting.2018.04.024 ER -
@article{ author = "Filipović, Vuk and Božić-Nedeljković, Biljana and Vukomanović, Marija and Tomić, Simonida", year = "2018", abstract = "Gelatin hydrogels have great potential in regenerative medicine but their weak mechanical properties are a major drawback for the load-bearing applications, such as scaffolds for tissue engineering. To overcome this deficiency, novel biodegradable hydrogels with improved mechanical properties were prepared by combining gelatine with 2-hydroxyethyl methacrylate (HEMA), using a double network synthetic procedure. The first, superporous and mechanically strong network, was obtained by free radical polymerization of HEMA at cryogenic temperature, in the presence of gelatin. Degradable poly (beta-amino ester) (PBAE) macromers of different chemical composition or molecular weight were used as crosslinkers to introduce hydrolytically labile bonds in PHEMA. The second gelatin network was formed by crosslinking gelatin with glutaraldehyde. For comparison, a set of biodegradable PHEMA networks was obtained by polymerization of HEMA at cryogenic temperature. All samples were characterized revealing that mechanical strength, swelling behavior and degradation rate as well as high biocompatibility of new IPNs are in accordance with values required for scaffolds in tissue engineering applications and that tuning of these properties is accomplished by simply using different PBAE macromers.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Polymer Testing", title = "Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers", pages = "278-270", volume = "68", doi = "10.1016/j.polymertesting.2018.04.024" }
Filipović, V., Božić-Nedeljković, B., Vukomanović, M.,& Tomić, S.. (2018). Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers. in Polymer Testing Elsevier Sci Ltd, Oxford., 68, 270-278. https://doi.org/10.1016/j.polymertesting.2018.04.024
Filipović V, Božić-Nedeljković B, Vukomanović M, Tomić S. Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers. in Polymer Testing. 2018;68:270-278. doi:10.1016/j.polymertesting.2018.04.024 .
Filipović, Vuk, Božić-Nedeljković, Biljana, Vukomanović, Marija, Tomić, Simonida, "Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers" in Polymer Testing, 68 (2018):270-278, https://doi.org/10.1016/j.polymertesting.2018.04.024 . .