Show simple item record

dc.creatorCarević, Milica
dc.creatorVukašinović-Sekulić, Maja
dc.creatorĆorović, Marija
dc.creatorRogniaux, Helene
dc.creatorRopartz, David
dc.creatorVeličković, Dušan
dc.creatorBezbradica, Dejan
dc.date.accessioned2021-03-10T13:46:31Z
dc.date.available2021-03-10T13:46:31Z
dc.date.issued2018
dc.identifier.issn1389-1723
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/3934
dc.description.abstractbeta-Galactosidase is an important industrial enzyme that catalyzes reaction of lactose hydrolysis and recently more interesting reaction of transgalactosylation, yielding a highly valuable group of prebiotic compounds named galacto-oligosaccharides (GOS). In this paper, parameters for achieving high yields of tailor-made GOS using crude beta-galactosidase obtained from Lactobacillus acidophilus ATCC 4356, probiotic bacteria regarded as safe for human consumption, were optimized. At the same time, detailed structural elucidation of obtained GOS was conducted, and it was concluded that beta-galactosidase from L acidophilus shows a particular specificity towards the formation of beta-(1 - gt 6) glycosidic bonds. In order to develop more stable and economically cost-effective preparation, crude enzyme was successfully immobilized on a methacrylic polymer carrier Lifetech ECR8409, leading to its simultaneous 2-fold purification. This immobilized preparation showed unchanged specificity towards the transgalactosylation reaction, thus yielding 86 WI GOS under the previously optimized conditions (lactose concentration 400 g/l in 0.1 M sodium phosphate buffer, pH 6.8 and temperature 50 degrees C).en
dc.publisherSoc Bioscience Bioengineering Japan, Osaka
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Bioscience and Bioengineering
dc.subjectbeta-Galactosidaseen
dc.subjectImmobilizationen
dc.subjectLactobacillus acidophilusen
dc.subjectGalacto-oligosaccharidesen
dc.subjectLifetech ECR resinsen
dc.titleEvaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilizationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage704
dc.citation.issue6
dc.citation.other126(6): 697-704
dc.citation.rankM22
dc.citation.spage697
dc.citation.volume126
dc.identifier.doi10.1016/j.jbiosc.2018.06.003
dc.identifier.pmid30401452
dc.identifier.rcubconv_5723
dc.identifier.scopus2-s2.0-85049305185
dc.identifier.wos000452933700005
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record