Brewers' spent grain and thin stillage as raw materials in l-(+)-lactic acid fermentation

2018
Authors
Radosavljević, Miloš
Pejin, Jelena
Kocić-Tanackov, Sunčica

Mladenović, Dragana

Djukić-Vuković, Aleksandra

Mojović, Ljiljana

Article (Published version)

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Brewer's spent grain (BSG) hydrolysates were used for l-(+)-lactic acid (LA) fermentation by Lactobacillus rhamnosus ATCC 7469. In this study the effect of the addition of various amounts of thin stillage (TS) in BSG hydrolysate on LA fermentation parameters were evaluated. TS addition significantly increased utilization of glucose by up to 43.0%. In batch fermentation the highest LA concentration and volumetric productivity of 31.0g/L, and 0.93g/L/h, respectively, were obtained with the addition of 50% TS. L. rhamnosus cell viability also increased with the addition of 50% TS (by 2.4%). TS addition significantly increased free amino nitrogen concentration (by up to 209%) which is important for bacterial growth. A strong positive correlation between free amino nitrogen and LA concentration was noted. Compared with the results obtained in the batch fermentation (50% TS), significantly higher LA concentration, yield and volumetric productivity (54.8, 1.9 and 4.0%, respectively) were achi...eved in fed-batch fermentation with glucose and TS addition. The results suggest that the combination of the by-products of brewing and bioethanol industries could be suitable for LA production.
Keywords:
lactic acid fermentation / brewer's spent grain / stillage / L. rhamnosus / by-product utilizationSource:
Journal of the Institute of Brewing, 2018, 124, 1, 23-30Publisher:
- Inst Brewing, London
Funding / projects:
- Production of lactic acid and probiotics on waste products of food and agricultural industry (RS-31017)
DOI: 10.1002/jib.462
ISSN: 0046-9750
WoS: 000425592200004
Scopus: 2-s2.0-85034271693
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Tehnološko-metalurški fakultetTY - JOUR AU - Radosavljević, Miloš AU - Pejin, Jelena AU - Kocić-Tanackov, Sunčica AU - Mladenović, Dragana AU - Djukić-Vuković, Aleksandra AU - Mojović, Ljiljana PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3955 AB - Brewer's spent grain (BSG) hydrolysates were used for l-(+)-lactic acid (LA) fermentation by Lactobacillus rhamnosus ATCC 7469. In this study the effect of the addition of various amounts of thin stillage (TS) in BSG hydrolysate on LA fermentation parameters were evaluated. TS addition significantly increased utilization of glucose by up to 43.0%. In batch fermentation the highest LA concentration and volumetric productivity of 31.0g/L, and 0.93g/L/h, respectively, were obtained with the addition of 50% TS. L. rhamnosus cell viability also increased with the addition of 50% TS (by 2.4%). TS addition significantly increased free amino nitrogen concentration (by up to 209%) which is important for bacterial growth. A strong positive correlation between free amino nitrogen and LA concentration was noted. Compared with the results obtained in the batch fermentation (50% TS), significantly higher LA concentration, yield and volumetric productivity (54.8, 1.9 and 4.0%, respectively) were achieved in fed-batch fermentation with glucose and TS addition. The results suggest that the combination of the by-products of brewing and bioethanol industries could be suitable for LA production. PB - Inst Brewing, London T2 - Journal of the Institute of Brewing T1 - Brewers' spent grain and thin stillage as raw materials in l-(+)-lactic acid fermentation EP - 30 IS - 1 SP - 23 VL - 124 DO - 10.1002/jib.462 ER -
@article{ author = "Radosavljević, Miloš and Pejin, Jelena and Kocić-Tanackov, Sunčica and Mladenović, Dragana and Djukić-Vuković, Aleksandra and Mojović, Ljiljana", year = "2018", abstract = "Brewer's spent grain (BSG) hydrolysates were used for l-(+)-lactic acid (LA) fermentation by Lactobacillus rhamnosus ATCC 7469. In this study the effect of the addition of various amounts of thin stillage (TS) in BSG hydrolysate on LA fermentation parameters were evaluated. TS addition significantly increased utilization of glucose by up to 43.0%. In batch fermentation the highest LA concentration and volumetric productivity of 31.0g/L, and 0.93g/L/h, respectively, were obtained with the addition of 50% TS. L. rhamnosus cell viability also increased with the addition of 50% TS (by 2.4%). TS addition significantly increased free amino nitrogen concentration (by up to 209%) which is important for bacterial growth. A strong positive correlation between free amino nitrogen and LA concentration was noted. Compared with the results obtained in the batch fermentation (50% TS), significantly higher LA concentration, yield and volumetric productivity (54.8, 1.9 and 4.0%, respectively) were achieved in fed-batch fermentation with glucose and TS addition. The results suggest that the combination of the by-products of brewing and bioethanol industries could be suitable for LA production.", publisher = "Inst Brewing, London", journal = "Journal of the Institute of Brewing", title = "Brewers' spent grain and thin stillage as raw materials in l-(+)-lactic acid fermentation", pages = "30-23", number = "1", volume = "124", doi = "10.1002/jib.462" }
Radosavljević, M., Pejin, J., Kocić-Tanackov, S., Mladenović, D., Djukić-Vuković, A.,& Mojović, L.. (2018). Brewers' spent grain and thin stillage as raw materials in l-(+)-lactic acid fermentation. in Journal of the Institute of Brewing Inst Brewing, London., 124(1), 23-30. https://doi.org/10.1002/jib.462
Radosavljević M, Pejin J, Kocić-Tanackov S, Mladenović D, Djukić-Vuković A, Mojović L. Brewers' spent grain and thin stillage as raw materials in l-(+)-lactic acid fermentation. in Journal of the Institute of Brewing. 2018;124(1):23-30. doi:10.1002/jib.462 .
Radosavljević, Miloš, Pejin, Jelena, Kocić-Tanackov, Sunčica, Mladenović, Dragana, Djukić-Vuković, Aleksandra, Mojović, Ljiljana, "Brewers' spent grain and thin stillage as raw materials in l-(+)-lactic acid fermentation" in Journal of the Institute of Brewing, 124, no. 1 (2018):23-30, https://doi.org/10.1002/jib.462 . .