Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.
Само за регистроване кориснике
2018
Аутори
Durdević, SanjaŠavikin, Katarina
Živković, Jelena Č.
Boehm, Volker
Stanojković, Tatjana
Damjanović, Ana
Petrović, Slobodan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malig...nant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50 gt 200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.
Кључне речи:
Pomegranate seed oil / Punicic acid / Tocopherols / alpha-TEAC assay / MTT assayИзвор:
Journal of Supercritical Fluids, 2018, 133, 225-232Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Традиционални и нови производи од плодова гајених и самониклих врста воћака и винове лозе и нус-продуката у преради, са посебним освртом на аутохтоне сорте: хемијска карактеризација и биолошки профил (RS-46013)
- Функционални физиолошки активни биљни материјали са додатом вредношћу за примену у фармацеутској и прехрамбеној индустрији (RS-45017)
- Модификатори биолошког одговора у физиолошким и патолошким стањима (RS-175011)
DOI: 10.1016/j.supflu.2017.10.021
ISSN: 0896-8446
WoS: 000426234000028
Scopus: 2-s2.0-85032260370
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Durdević, Sanja AU - Šavikin, Katarina AU - Živković, Jelena Č. AU - Boehm, Volker AU - Stanojković, Tatjana AU - Damjanović, Ana AU - Petrović, Slobodan PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3961 AB - Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50 gt 200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Supercritical Fluids T1 - Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L. EP - 232 SP - 225 VL - 133 DO - 10.1016/j.supflu.2017.10.021 ER -
@article{ author = "Durdević, Sanja and Šavikin, Katarina and Živković, Jelena Č. and Boehm, Volker and Stanojković, Tatjana and Damjanović, Ana and Petrović, Slobodan", year = "2018", abstract = "Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50 gt 200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Supercritical Fluids", title = "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.", pages = "232-225", volume = "133", doi = "10.1016/j.supflu.2017.10.021" }
Durdević, S., Šavikin, K., Živković, J. Č., Boehm, V., Stanojković, T., Damjanović, A.,& Petrović, S.. (2018). Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids Elsevier Science Bv, Amsterdam., 133, 225-232. https://doi.org/10.1016/j.supflu.2017.10.021
Durdević S, Šavikin K, Živković JČ, Boehm V, Stanojković T, Damjanović A, Petrović S. Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids. 2018;133:225-232. doi:10.1016/j.supflu.2017.10.021 .
Durdević, Sanja, Šavikin, Katarina, Živković, Jelena Č., Boehm, Volker, Stanojković, Tatjana, Damjanović, Ana, Petrović, Slobodan, "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L." in Journal of Supercritical Fluids, 133 (2018):225-232, https://doi.org/10.1016/j.supflu.2017.10.021 . .