Lactic acid production on molasses enriched potato stillage by Lactobacillus paracasei immobilized onto agro-industrial waste supports
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2018
Authors
Mladenović, DraganaPejin, Jelena
Kocić-Tanackov, Sunčica
Radovanović, Željko
Đukić-Vuković, Aleksandra
Mojović, Ljiljana
Article (Published version)
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In this study, the production of lactic acid (LA) on sugar beet molasses enriched potato stillage by immobilized Lactobacillus paracasei NRRL B-4564 was examined. Three agro-industrial materials, such as sunflower seed hull (SSH), brewers' spent grain (BSG), and sugar beet pulp (SBP) were studied as carriers for cell immobilization. The carriers were physically characterized in terms of water adsorption index (WAI), critical humidity point (CHP) and porosity. Further, the stability and efficiency of the immobilized biocatalysts were evaluated in repeated batch fermentation of molasses enriched potato stillage and compared with free cell system. A strong cell attachment onto agro-industrial supports allowed easy separation from the fermentation media and efficient biocatalyst reuse in five successive batch cycles. The highest cell number attached on the support surface during the fermentation was detected for material with higher WAI and lower CHP. Porosimetry measurements showed that t...he attachment of L. paracasei cells to support materials and overall LA productivities achieved with different immobilized biocatalysts were not determined by the support porosity and surface morphology, but with the material characteristics such as electrostatic charge, chemical composition and hydrophilicity. LA productivity of 1.48 g/L h, maximal LA concentration of 80.10 g/L and average yield coefficient of 0.97 g/g were achieved in fermentation of molasses enriched potato stillage using SBP as a support material, followed by BSG and SSH. The studied approach showed to be an interesting alternative strategy for increasing the LA productivities on low-cost and abundant substrate.
Keywords:
Lactic acid / Sunflower seed hull / Spent grain / Sugar beet pulp / Microbial immobilization / Lactobacillus paracasei NRRL B-4564Source:
Industrial Crops and Products, 2018, 124, 142-148Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Production of lactic acid and probiotics on waste products of food and agricultural industry (RS-MESTD-Technological Development (TD or TR)-31017)
- Scientific and Technological Collaboration of Republic of Serbia and PR China [I-1/2018]
DOI: 10.1016/j.indcrop.2018.07.081
ISSN: 0926-6690
WoS: 000447569100018
Scopus: 2-s2.0-85050889859
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Tehnološko-metalurški fakultetTY - JOUR AU - Mladenović, Dragana AU - Pejin, Jelena AU - Kocić-Tanackov, Sunčica AU - Radovanović, Željko AU - Đukić-Vuković, Aleksandra AU - Mojović, Ljiljana PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3984 AB - In this study, the production of lactic acid (LA) on sugar beet molasses enriched potato stillage by immobilized Lactobacillus paracasei NRRL B-4564 was examined. Three agro-industrial materials, such as sunflower seed hull (SSH), brewers' spent grain (BSG), and sugar beet pulp (SBP) were studied as carriers for cell immobilization. The carriers were physically characterized in terms of water adsorption index (WAI), critical humidity point (CHP) and porosity. Further, the stability and efficiency of the immobilized biocatalysts were evaluated in repeated batch fermentation of molasses enriched potato stillage and compared with free cell system. A strong cell attachment onto agro-industrial supports allowed easy separation from the fermentation media and efficient biocatalyst reuse in five successive batch cycles. The highest cell number attached on the support surface during the fermentation was detected for material with higher WAI and lower CHP. Porosimetry measurements showed that the attachment of L. paracasei cells to support materials and overall LA productivities achieved with different immobilized biocatalysts were not determined by the support porosity and surface morphology, but with the material characteristics such as electrostatic charge, chemical composition and hydrophilicity. LA productivity of 1.48 g/L h, maximal LA concentration of 80.10 g/L and average yield coefficient of 0.97 g/g were achieved in fermentation of molasses enriched potato stillage using SBP as a support material, followed by BSG and SSH. The studied approach showed to be an interesting alternative strategy for increasing the LA productivities on low-cost and abundant substrate. PB - Elsevier Science Bv, Amsterdam T2 - Industrial Crops and Products T1 - Lactic acid production on molasses enriched potato stillage by Lactobacillus paracasei immobilized onto agro-industrial waste supports EP - 148 SP - 142 VL - 124 DO - 10.1016/j.indcrop.2018.07.081 ER -
@article{ author = "Mladenović, Dragana and Pejin, Jelena and Kocić-Tanackov, Sunčica and Radovanović, Željko and Đukić-Vuković, Aleksandra and Mojović, Ljiljana", year = "2018", abstract = "In this study, the production of lactic acid (LA) on sugar beet molasses enriched potato stillage by immobilized Lactobacillus paracasei NRRL B-4564 was examined. Three agro-industrial materials, such as sunflower seed hull (SSH), brewers' spent grain (BSG), and sugar beet pulp (SBP) were studied as carriers for cell immobilization. The carriers were physically characterized in terms of water adsorption index (WAI), critical humidity point (CHP) and porosity. Further, the stability and efficiency of the immobilized biocatalysts were evaluated in repeated batch fermentation of molasses enriched potato stillage and compared with free cell system. A strong cell attachment onto agro-industrial supports allowed easy separation from the fermentation media and efficient biocatalyst reuse in five successive batch cycles. The highest cell number attached on the support surface during the fermentation was detected for material with higher WAI and lower CHP. Porosimetry measurements showed that the attachment of L. paracasei cells to support materials and overall LA productivities achieved with different immobilized biocatalysts were not determined by the support porosity and surface morphology, but with the material characteristics such as electrostatic charge, chemical composition and hydrophilicity. LA productivity of 1.48 g/L h, maximal LA concentration of 80.10 g/L and average yield coefficient of 0.97 g/g were achieved in fermentation of molasses enriched potato stillage using SBP as a support material, followed by BSG and SSH. The studied approach showed to be an interesting alternative strategy for increasing the LA productivities on low-cost and abundant substrate.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Industrial Crops and Products", title = "Lactic acid production on molasses enriched potato stillage by Lactobacillus paracasei immobilized onto agro-industrial waste supports", pages = "148-142", volume = "124", doi = "10.1016/j.indcrop.2018.07.081" }
Mladenović, D., Pejin, J., Kocić-Tanackov, S., Radovanović, Ž., Đukić-Vuković, A.,& Mojović, L.. (2018). Lactic acid production on molasses enriched potato stillage by Lactobacillus paracasei immobilized onto agro-industrial waste supports. in Industrial Crops and Products Elsevier Science Bv, Amsterdam., 124, 142-148. https://doi.org/10.1016/j.indcrop.2018.07.081
Mladenović D, Pejin J, Kocić-Tanackov S, Radovanović Ž, Đukić-Vuković A, Mojović L. Lactic acid production on molasses enriched potato stillage by Lactobacillus paracasei immobilized onto agro-industrial waste supports. in Industrial Crops and Products. 2018;124:142-148. doi:10.1016/j.indcrop.2018.07.081 .
Mladenović, Dragana, Pejin, Jelena, Kocić-Tanackov, Sunčica, Radovanović, Željko, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, "Lactic acid production on molasses enriched potato stillage by Lactobacillus paracasei immobilized onto agro-industrial waste supports" in Industrial Crops and Products, 124 (2018):142-148, https://doi.org/10.1016/j.indcrop.2018.07.081 . .