Valorization of damaged rice grains: Optimization of bioethanol production by waste brewer's yeast using an amylolytic potential from the Paenibacillus chitinolyticus CKS1
Abstract
Damaged grains that are not useful for food or feed are abundant and cheap. The main objective of this paper was to valorize damaged rice grains for bioethanol production using presacchararification step followed by simultaneous saccharification and fermentation (SSF) by using waste brewer's yeast. Presacchararification step was carried out using crude amylases that produce naturale isolate Paenibacillus chitinolyticus CKS1. Maltose, a fermentable sugar, was the main product during presaccharification. Statistical design, central composite design (CCD), was used for optimization of process parameters for ethanol production, yeast cell yield and utilized sugars. Under optimal conditions 0.5% malt extract, 3.84% inoculum and 2.76 days of fermentation maximum ethanol production of 4.69% was achieved. The obtained ethanol concentration was in a good correlation with yeast cell yield and utilized reducing sugars. The results obtained in this study indicate that the economical production of ...ethanol could be achieved using cheap waste materials also using low cost enzymes derivated from natural bacterial isolates.
Keywords:
Ethanol / Damaged rice / Amylases / Waste brewer's yeast / Statistical optimizationSource:
Fuel, 2018, 224, 591-599Publisher:
- Elsevier Sci Ltd, Oxford
Funding / projects:
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-31035)
DOI: 10.1016/j.fuel.2018.03.135
ISSN: 0016-2361
WoS: 000432920900059
Scopus: 2-s2.0-85044154641
Collections
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Mihajlovski, Katarina AU - Radovanović, Željko AU - Carević, Milica AU - Dimitrijević-Branković, Suzana PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4004 AB - Damaged grains that are not useful for food or feed are abundant and cheap. The main objective of this paper was to valorize damaged rice grains for bioethanol production using presacchararification step followed by simultaneous saccharification and fermentation (SSF) by using waste brewer's yeast. Presacchararification step was carried out using crude amylases that produce naturale isolate Paenibacillus chitinolyticus CKS1. Maltose, a fermentable sugar, was the main product during presaccharification. Statistical design, central composite design (CCD), was used for optimization of process parameters for ethanol production, yeast cell yield and utilized sugars. Under optimal conditions 0.5% malt extract, 3.84% inoculum and 2.76 days of fermentation maximum ethanol production of 4.69% was achieved. The obtained ethanol concentration was in a good correlation with yeast cell yield and utilized reducing sugars. The results obtained in this study indicate that the economical production of ethanol could be achieved using cheap waste materials also using low cost enzymes derivated from natural bacterial isolates. PB - Elsevier Sci Ltd, Oxford T2 - Fuel T1 - Valorization of damaged rice grains: Optimization of bioethanol production by waste brewer's yeast using an amylolytic potential from the Paenibacillus chitinolyticus CKS1 EP - 599 SP - 591 VL - 224 DO - 10.1016/j.fuel.2018.03.135 ER -
@article{ author = "Mihajlovski, Katarina and Radovanović, Željko and Carević, Milica and Dimitrijević-Branković, Suzana", year = "2018", abstract = "Damaged grains that are not useful for food or feed are abundant and cheap. The main objective of this paper was to valorize damaged rice grains for bioethanol production using presacchararification step followed by simultaneous saccharification and fermentation (SSF) by using waste brewer's yeast. Presacchararification step was carried out using crude amylases that produce naturale isolate Paenibacillus chitinolyticus CKS1. Maltose, a fermentable sugar, was the main product during presaccharification. Statistical design, central composite design (CCD), was used for optimization of process parameters for ethanol production, yeast cell yield and utilized sugars. Under optimal conditions 0.5% malt extract, 3.84% inoculum and 2.76 days of fermentation maximum ethanol production of 4.69% was achieved. The obtained ethanol concentration was in a good correlation with yeast cell yield and utilized reducing sugars. The results obtained in this study indicate that the economical production of ethanol could be achieved using cheap waste materials also using low cost enzymes derivated from natural bacterial isolates.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Fuel", title = "Valorization of damaged rice grains: Optimization of bioethanol production by waste brewer's yeast using an amylolytic potential from the Paenibacillus chitinolyticus CKS1", pages = "599-591", volume = "224", doi = "10.1016/j.fuel.2018.03.135" }
Mihajlovski, K., Radovanović, Ž., Carević, M.,& Dimitrijević-Branković, S.. (2018). Valorization of damaged rice grains: Optimization of bioethanol production by waste brewer's yeast using an amylolytic potential from the Paenibacillus chitinolyticus CKS1. in Fuel Elsevier Sci Ltd, Oxford., 224, 591-599. https://doi.org/10.1016/j.fuel.2018.03.135
Mihajlovski K, Radovanović Ž, Carević M, Dimitrijević-Branković S. Valorization of damaged rice grains: Optimization of bioethanol production by waste brewer's yeast using an amylolytic potential from the Paenibacillus chitinolyticus CKS1. in Fuel. 2018;224:591-599. doi:10.1016/j.fuel.2018.03.135 .
Mihajlovski, Katarina, Radovanović, Željko, Carević, Milica, Dimitrijević-Branković, Suzana, "Valorization of damaged rice grains: Optimization of bioethanol production by waste brewer's yeast using an amylolytic potential from the Paenibacillus chitinolyticus CKS1" in Fuel, 224 (2018):591-599, https://doi.org/10.1016/j.fuel.2018.03.135 . .