Show simple item record

dc.creatorGvozdenović-Jeremić, Jelena
dc.creatorVert-Wong, Ekaterina
dc.creatorMojović, Ljiljana
dc.date.accessioned2021-03-10T13:54:28Z
dc.date.available2021-03-10T13:54:28Z
dc.date.issued2019
dc.identifier.issn0352-5139
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/4057
dc.description.abstractPathological formation of bone in non-skeletal soft tissues or heterotopic ossification (HO), for which there is currently no effective treatment, is considered to be mediated by activation of Hedgehog (Hh) signaling pathway. Moreover, the biochemical mechanism of this pathological process is not fully understood. Here, we tested the efficacy of three chemical inhibitors of the Hh signaling pathway, arsenic trioxide (ATO), lovastatin (Lov) and cholecalciferol (Vitamin D) to hamper differentiation of mesenchymal stem cells (MSC) into osteoblasts or osteogenesis. Each of the three Hh inhibitors potently decreased alkaline phosphatase activity, suggesting effective suppression of osteogenic activity in Hh-impaired MSC. Gene expression analysis revealed a significant reduction in mRNA levels of chief Hh signaling marker, Gli1, following administration of Hh small molecule inhibitors. A functional link between Hedgehog and osteogenesis in native MSC cells is further established in studies involving the mix of three Hh inhibitors acting at different checkpoints of the Hh signaling pathway. Thus, a combination of small molecule inhibitors of the Hh pathway at their lower concentrations could be a novel approach for HO prophylaxis with increased efficacy and potentially reduced side effects.en
dc.publisherSrpsko hemijsko društvo, Beograd
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceJournal of the Serbian Chemical Society
dc.subjectheterotopic ossificationen
dc.subjectHedgehog inhibitorsen
dc.subjectcombination therapyen
dc.subjectmesenchymal stem cellsen
dc.titleAntiosteogenic effect of arsenic trioxide, cholecalciferol, lovastatin or their combination in vitroen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage960
dc.citation.issue9
dc.citation.other84(9): 951-960
dc.citation.rankM23
dc.citation.spage951
dc.citation.volume84
dc.identifier.doi10.2298/JSC190304060G
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/1798/4054.pdf
dc.identifier.rcubconv_5944
dc.identifier.scopus2-s2.0-85072950364
dc.identifier.wos000489023300004
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record