Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production
Samo za registrovane korisnike
2019
Autori
Buntić, AnetaMilić, Marija
Stajković-Srbinović, Olivera S.
Rasulić, Nataša V.
Delić, Dušica I.
Mihajlovski, Katarina
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl ce...llulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.
Ključne reči:
Avicelase / Carboxymethyl cellulase / Lignocellulose / Response surface method / RhizobiumIzvor:
International Journal of Environmental Science and Technology, 2019, 16, 10, 5881-5890Izdavač:
- Springer, New York
Finansiranje / projekti:
- Primena biotehnoloških metoda u održivom iskorišćenju nus-proizvoda agroindustrije (RS-MESTD-Technological Development (TD or TR)-31035)
- Proučavanje uticaja kvaliteta zemljišta i voda za navodnjavanje na efikasniju proizvodnju poljoprivrednih kultura i očuvanje životne sredine (RS-MESTD-Technological Development (TD or TR)-37006)
DOI: 10.1007/s13762-019-02230-9
ISSN: 1735-1472
WoS: 000486155600043
Scopus: 2-s2.0-85060716746
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Buntić, Aneta AU - Milić, Marija AU - Stajković-Srbinović, Olivera S. AU - Rasulić, Nataša V. AU - Delić, Dušica I. AU - Mihajlovski, Katarina PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4075 AB - This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste. PB - Springer, New York T2 - International Journal of Environmental Science and Technology T1 - Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production EP - 5890 IS - 10 SP - 5881 VL - 16 DO - 10.1007/s13762-019-02230-9 ER -
@article{ author = "Buntić, Aneta and Milić, Marija and Stajković-Srbinović, Olivera S. and Rasulić, Nataša V. and Delić, Dušica I. and Mihajlovski, Katarina", year = "2019", abstract = "This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.", publisher = "Springer, New York", journal = "International Journal of Environmental Science and Technology", title = "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production", pages = "5890-5881", number = "10", volume = "16", doi = "10.1007/s13762-019-02230-9" }
Buntić, A., Milić, M., Stajković-Srbinović, O. S., Rasulić, N. V., Delić, D. I.,& Mihajlovski, K.. (2019). Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology Springer, New York., 16(10), 5881-5890. https://doi.org/10.1007/s13762-019-02230-9
Buntić A, Milić M, Stajković-Srbinović OS, Rasulić NV, Delić DI, Mihajlovski K. Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology. 2019;16(10):5881-5890. doi:10.1007/s13762-019-02230-9 .
Buntić, Aneta, Milić, Marija, Stajković-Srbinović, Olivera S., Rasulić, Nataša V., Delić, Dušica I., Mihajlovski, Katarina, "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production" in International Journal of Environmental Science and Technology, 16, no. 10 (2019):5881-5890, https://doi.org/10.1007/s13762-019-02230-9 . .