Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol
Само за регистроване кориснике
2019
Аутори
Jovanović, AleksandraBalanč, Bojana
Đorđević, Verica
Ota, Ajda
Skrt, Mihaela
Šavikin, Katarina
Bugarski, Branko
Nedović, Viktor
Poklar-Ulrih, Nataša
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather... than by enzymes activity.
Кључне речи:
Gentisic acid / Liposomes / beta-sitosterol / Release study / Membrane fluidity / PhosphatidylcholinesИзвор:
Colloids and Surfaces B-Biointerfaces, 2019, 183Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Slovenian Research AgencySlovenian Research Agency - Slovenia [P4-0121]
- bilateral project Serbia
- bilateral project Slovenia
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Традиционални и нови производи од плодова гајених и самониклих врста воћака и винове лозе и нус-продуката у преради, са посебним освртом на аутохтоне сорте: хемијска карактеризација и биолошки профил (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46013)
DOI: 10.1016/j.colsurfb.2019.110422
ISSN: 0927-7765
PubMed: 31437609
WoS: 000494048600029
Scopus: 2-s2.0-85070688519
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Jovanović, Aleksandra AU - Balanč, Bojana AU - Đorđević, Verica AU - Ota, Ajda AU - Skrt, Mihaela AU - Šavikin, Katarina AU - Bugarski, Branko AU - Nedović, Viktor AU - Poklar-Ulrih, Nataša PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4088 AB - Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity. PB - Elsevier, Amsterdam T2 - Colloids and Surfaces B-Biointerfaces T1 - Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol VL - 183 DO - 10.1016/j.colsurfb.2019.110422 ER -
@article{ author = "Jovanović, Aleksandra and Balanč, Bojana and Đorđević, Verica and Ota, Ajda and Skrt, Mihaela and Šavikin, Katarina and Bugarski, Branko and Nedović, Viktor and Poklar-Ulrih, Nataša", year = "2019", abstract = "Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity.", publisher = "Elsevier, Amsterdam", journal = "Colloids and Surfaces B-Biointerfaces", title = "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol", volume = "183", doi = "10.1016/j.colsurfb.2019.110422" }
Jovanović, A., Balanč, B., Đorđević, V., Ota, A., Skrt, M., Šavikin, K., Bugarski, B., Nedović, V.,& Poklar-Ulrih, N.. (2019). Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces Elsevier, Amsterdam., 183. https://doi.org/10.1016/j.colsurfb.2019.110422
Jovanović A, Balanč B, Đorđević V, Ota A, Skrt M, Šavikin K, Bugarski B, Nedović V, Poklar-Ulrih N. Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces. 2019;183. doi:10.1016/j.colsurfb.2019.110422 .
Jovanović, Aleksandra, Balanč, Bojana, Đorđević, Verica, Ota, Ajda, Skrt, Mihaela, Šavikin, Katarina, Bugarski, Branko, Nedović, Viktor, Poklar-Ulrih, Nataša, "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol" in Colloids and Surfaces B-Biointerfaces, 183 (2019), https://doi.org/10.1016/j.colsurfb.2019.110422 . .