Application of agro-industrial by-products for the production of lactic acid was studied in this paper. Brewer's spent grain (BSG), malt rootlets (MR), brewer's yeast (BY), and soy lecithin (SL) were used as raw materials in l-(+)-LA fermentation by free and immobilized Lactobacillus rhamnosus ATCC 7469. The BSG, solid remains after BSG and MR hydrolysis (BSGMRSR), and MR were evaluated as carriers for batch and repeated batch fermentations with immobilized cells. During batch fermentations with immobilized cells, high cell viability (10 to 11logCFU/g) was achieved on all carriers. In batch fermentation with BSG as a carrier, the highest LA yield of 93.79% and volumetric productivity of 1.15g/L/h were obtained. Furthermore, very high LA yield (95.46%), volumetric productivity (1.98g/L/h) and L. rhamnosus viability (11.5logCFU/g) were achieved in repeated batch fermentations with the cells immobilized on this carrier. The immobilized cells showed high survival rate (94-95%) during expos...ure to simulated gut condition. Based on the analysis of BSGMRSR, and BY solid remains, and on in vitro evaluation of the probiotic characteristics of immobilized cells, it was observed that they could satisfy the recommendations for high-quality feed preparation.
TY - JOUR
AU - Radosavljević, Miloš
AU - Pejin, Jelena
AU - Pribić, Milana
AU - Kocić-Tanackov, Sunčica
AU - Romanić, Ranko
AU - Mladenović, Dragana
AU - Đukić-Vuković, Aleksandra
AU - Mojović, Ljiljana
PY - 2019
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4149
AB - Application of agro-industrial by-products for the production of lactic acid was studied in this paper. Brewer's spent grain (BSG), malt rootlets (MR), brewer's yeast (BY), and soy lecithin (SL) were used as raw materials in l-(+)-LA fermentation by free and immobilized Lactobacillus rhamnosus ATCC 7469. The BSG, solid remains after BSG and MR hydrolysis (BSGMRSR), and MR were evaluated as carriers for batch and repeated batch fermentations with immobilized cells. During batch fermentations with immobilized cells, high cell viability (10 to 11logCFU/g) was achieved on all carriers. In batch fermentation with BSG as a carrier, the highest LA yield of 93.79% and volumetric productivity of 1.15g/L/h were obtained. Furthermore, very high LA yield (95.46%), volumetric productivity (1.98g/L/h) and L. rhamnosus viability (11.5logCFU/g) were achieved in repeated batch fermentations with the cells immobilized on this carrier. The immobilized cells showed high survival rate (94-95%) during exposure to simulated gut condition. Based on the analysis of BSGMRSR, and BY solid remains, and on in vitro evaluation of the probiotic characteristics of immobilized cells, it was observed that they could satisfy the recommendations for high-quality feed preparation.
PB - Springer, New York
T2 - Applied Microbiology and Biotechnology
T1 - Utilization of brewing and malting by-products as carrier and raw materials in l-(+)-lactic acid production and feed application
EP - 3013
IS - 7
SP - 3001
VL - 103
DO - 10.1007/s00253-019-09683-5
ER -
@article{
author = "Radosavljević, Miloš and Pejin, Jelena and Pribić, Milana and Kocić-Tanackov, Sunčica and Romanić, Ranko and Mladenović, Dragana and Đukić-Vuković, Aleksandra and Mojović, Ljiljana",
year = "2019",
abstract = "Application of agro-industrial by-products for the production of lactic acid was studied in this paper. Brewer's spent grain (BSG), malt rootlets (MR), brewer's yeast (BY), and soy lecithin (SL) were used as raw materials in l-(+)-LA fermentation by free and immobilized Lactobacillus rhamnosus ATCC 7469. The BSG, solid remains after BSG and MR hydrolysis (BSGMRSR), and MR were evaluated as carriers for batch and repeated batch fermentations with immobilized cells. During batch fermentations with immobilized cells, high cell viability (10 to 11logCFU/g) was achieved on all carriers. In batch fermentation with BSG as a carrier, the highest LA yield of 93.79% and volumetric productivity of 1.15g/L/h were obtained. Furthermore, very high LA yield (95.46%), volumetric productivity (1.98g/L/h) and L. rhamnosus viability (11.5logCFU/g) were achieved in repeated batch fermentations with the cells immobilized on this carrier. The immobilized cells showed high survival rate (94-95%) during exposure to simulated gut condition. Based on the analysis of BSGMRSR, and BY solid remains, and on in vitro evaluation of the probiotic characteristics of immobilized cells, it was observed that they could satisfy the recommendations for high-quality feed preparation.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Utilization of brewing and malting by-products as carrier and raw materials in l-(+)-lactic acid production and feed application",
pages = "3013-3001",
number = "7",
volume = "103",
doi = "10.1007/s00253-019-09683-5"
}
Radosavljević, M., Pejin, J., Pribić, M., Kocić-Tanackov, S., Romanić, R., Mladenović, D., Đukić-Vuković, A.,& Mojović, L.. (2019). Utilization of brewing and malting by-products as carrier and raw materials in l-(+)-lactic acid production and feed application. in Applied Microbiology and Biotechnology
Springer, New York., 103(7), 3001-3013.
https://doi.org/10.1007/s00253-019-09683-5
Radosavljević M, Pejin J, Pribić M, Kocić-Tanackov S, Romanić R, Mladenović D, Đukić-Vuković A, Mojović L. Utilization of brewing and malting by-products as carrier and raw materials in l-(+)-lactic acid production and feed application. in Applied Microbiology and Biotechnology. 2019;103(7):3001-3013.
doi:10.1007/s00253-019-09683-5 .
Radosavljević, Miloš, Pejin, Jelena, Pribić, Milana, Kocić-Tanackov, Sunčica, Romanić, Ranko, Mladenović, Dragana, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, "Utilization of brewing and malting by-products as carrier and raw materials in l-(+)-lactic acid production and feed application" in Applied Microbiology and Biotechnology, 103, no. 7 (2019):3001-3013,
https://doi.org/10.1007/s00253-019-09683-5 . .
