Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of alpha-Arbutin
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alpha-Arbutin (4-hydroquinone-alpha-D-glucopyranoside), an effective skin-lightening agent due to its considerable inhibitory effect on human tyrosinase activity, is widely used in the pharmaceutical and cosmetic industries. Recently, alpha-arbutin was prepared through transglucosylation of hydroquinone using microbial glycosyltransferases as catalysts. However, the low yield and prolonged reaction time of the biotransformation process of alpha-arbutin production limited its industrial application. In this work, an amylosucrase (ASase) from Xanthomonas campestris pv. campestris str. ATCC 33913 (XcAS) was expressed efficiently in Escherichia coli JM109. The catalytic property of the purified XcAS for the synthesis of alpha-arbutin was tested. The recombinant strain was applied for highly efficient synthesis of a-arbutin using sucrose and hydroquinone as glucosyl donor and acceptor, respectively. By optimizing the biotransformation conditions and applying a fed-batch strategy, the final ...production yield and conversion rate of alpha-arbutin reached 60.9 g/L and 95.5%, respectively, which is the highest reported yield by engineered strains. Compared to the highest reported value ( lt 1.4 g/L/h), our productivity (7.6 g/L/h) was improved more than five-fold. This work represents an efficient and rapid method for alpha-arbutin production with potential industrial applications.
Кључне речи:
Amylosucrase / Xanthomonas campestris pv. Campestris / alpha-arbutin / enzymatic properties / whole-cell biocatalyst / fed-batch strategyИзвор:
Catalysts, 2019, 9, 1Издавач:
- MDPI, Basel
Финансирање / пројекти:
- State Key Project of Research and Development Plan [2017YFE0112700]
- National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41106125]
DOI: 10.3390/catal9010005
ISSN: 2073-4344
WoS: 000459732000005
Scopus: 2-s2.0-85059365385
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Yang, Chengyu AU - Fan, Weiming AU - Zhang, Ruijie AU - Shi, Jiping AU - Knežević-Jugović, Zorica AU - Zhang, Baoguo PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4183 AB - alpha-Arbutin (4-hydroquinone-alpha-D-glucopyranoside), an effective skin-lightening agent due to its considerable inhibitory effect on human tyrosinase activity, is widely used in the pharmaceutical and cosmetic industries. Recently, alpha-arbutin was prepared through transglucosylation of hydroquinone using microbial glycosyltransferases as catalysts. However, the low yield and prolonged reaction time of the biotransformation process of alpha-arbutin production limited its industrial application. In this work, an amylosucrase (ASase) from Xanthomonas campestris pv. campestris str. ATCC 33913 (XcAS) was expressed efficiently in Escherichia coli JM109. The catalytic property of the purified XcAS for the synthesis of alpha-arbutin was tested. The recombinant strain was applied for highly efficient synthesis of a-arbutin using sucrose and hydroquinone as glucosyl donor and acceptor, respectively. By optimizing the biotransformation conditions and applying a fed-batch strategy, the final production yield and conversion rate of alpha-arbutin reached 60.9 g/L and 95.5%, respectively, which is the highest reported yield by engineered strains. Compared to the highest reported value ( lt 1.4 g/L/h), our productivity (7.6 g/L/h) was improved more than five-fold. This work represents an efficient and rapid method for alpha-arbutin production with potential industrial applications. PB - MDPI, Basel T2 - Catalysts T1 - Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of alpha-Arbutin IS - 1 VL - 9 DO - 10.3390/catal9010005 ER -
@article{ author = "Yang, Chengyu and Fan, Weiming and Zhang, Ruijie and Shi, Jiping and Knežević-Jugović, Zorica and Zhang, Baoguo", year = "2019", abstract = "alpha-Arbutin (4-hydroquinone-alpha-D-glucopyranoside), an effective skin-lightening agent due to its considerable inhibitory effect on human tyrosinase activity, is widely used in the pharmaceutical and cosmetic industries. Recently, alpha-arbutin was prepared through transglucosylation of hydroquinone using microbial glycosyltransferases as catalysts. However, the low yield and prolonged reaction time of the biotransformation process of alpha-arbutin production limited its industrial application. In this work, an amylosucrase (ASase) from Xanthomonas campestris pv. campestris str. ATCC 33913 (XcAS) was expressed efficiently in Escherichia coli JM109. The catalytic property of the purified XcAS for the synthesis of alpha-arbutin was tested. The recombinant strain was applied for highly efficient synthesis of a-arbutin using sucrose and hydroquinone as glucosyl donor and acceptor, respectively. By optimizing the biotransformation conditions and applying a fed-batch strategy, the final production yield and conversion rate of alpha-arbutin reached 60.9 g/L and 95.5%, respectively, which is the highest reported yield by engineered strains. Compared to the highest reported value ( lt 1.4 g/L/h), our productivity (7.6 g/L/h) was improved more than five-fold. This work represents an efficient and rapid method for alpha-arbutin production with potential industrial applications.", publisher = "MDPI, Basel", journal = "Catalysts", title = "Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of alpha-Arbutin", number = "1", volume = "9", doi = "10.3390/catal9010005" }
Yang, C., Fan, W., Zhang, R., Shi, J., Knežević-Jugović, Z.,& Zhang, B.. (2019). Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of alpha-Arbutin. in Catalysts MDPI, Basel., 9(1). https://doi.org/10.3390/catal9010005
Yang C, Fan W, Zhang R, Shi J, Knežević-Jugović Z, Zhang B. Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of alpha-Arbutin. in Catalysts. 2019;9(1). doi:10.3390/catal9010005 .
Yang, Chengyu, Fan, Weiming, Zhang, Ruijie, Shi, Jiping, Knežević-Jugović, Zorica, Zhang, Baoguo, "Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of alpha-Arbutin" in Catalysts, 9, no. 1 (2019), https://doi.org/10.3390/catal9010005 . .