Show simple item record

dc.creatorYang, Chengyu
dc.creatorFan, Weiming
dc.creatorZhang, Ruijie
dc.creatorShi, Jiping
dc.creatorKnežević-Jugović, Zorica
dc.creatorZhang, Baoguo
dc.date.accessioned2021-03-10T14:02:30Z
dc.date.available2021-03-10T14:02:30Z
dc.date.issued2019
dc.identifier.issn2073-4344
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/4183
dc.description.abstractalpha-Arbutin (4-hydroquinone-alpha-D-glucopyranoside), an effective skin-lightening agent due to its considerable inhibitory effect on human tyrosinase activity, is widely used in the pharmaceutical and cosmetic industries. Recently, alpha-arbutin was prepared through transglucosylation of hydroquinone using microbial glycosyltransferases as catalysts. However, the low yield and prolonged reaction time of the biotransformation process of alpha-arbutin production limited its industrial application. In this work, an amylosucrase (ASase) from Xanthomonas campestris pv. campestris str. ATCC 33913 (XcAS) was expressed efficiently in Escherichia coli JM109. The catalytic property of the purified XcAS for the synthesis of alpha-arbutin was tested. The recombinant strain was applied for highly efficient synthesis of a-arbutin using sucrose and hydroquinone as glucosyl donor and acceptor, respectively. By optimizing the biotransformation conditions and applying a fed-batch strategy, the final production yield and conversion rate of alpha-arbutin reached 60.9 g/L and 95.5%, respectively, which is the highest reported yield by engineered strains. Compared to the highest reported value ( lt 1.4 g/L/h), our productivity (7.6 g/L/h) was improved more than five-fold. This work represents an efficient and rapid method for alpha-arbutin production with potential industrial applications.en
dc.publisherMDPI, Basel
dc.relationState Key Project of Research and Development Plan [2017YFE0112700]
dc.relationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41106125]
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceCatalysts
dc.subjectAmylosucraseen
dc.subjectXanthomonas campestris pv. Campestrisen
dc.subjectalpha-arbutinen
dc.subjectenzymatic propertiesen
dc.subjectwhole-cell biocatalysten
dc.subjectfed-batch strategyen
dc.titleStudy on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of alpha-Arbutinen
dc.typearticle
dc.rights.licenseBY
dc.citation.issue1
dc.citation.other9(1): -
dc.citation.rankM22
dc.citation.volume9
dc.identifier.doi10.3390/catal9010005
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/10012/catalysts-09-00005-v2.pdf
dc.identifier.rcubconv_5798
dc.identifier.scopus2-s2.0-85059365385
dc.identifier.wos000459732000005
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record