Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate
Само за регистроване кориснике
2019
Аутори
Mladenović, DraganaPejin, Jelena
Kocić-Tanackov, Sunčica
Đukić-Vuković, Aleksandra
Mojović, Ljiljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to perform the adaptation of Lactobacillus paracasei NRRL B-4564 to substrate through adaptive evolution in order to ensure intensive substrate utilization and enhanced L (+)-lactic acid (LA) production on molasses-enriched potato stillage. To evaluate the strain response to environmental conditions exposed during the adaptation process and to select the best adapted cells, the antioxidant activity and LA-producing capability were assessed in batch fermentation. The most promising adapted strain was further used in a pulsed fed-batch mode. Among three selected adapted strains, L. paracasei A-22 showed considerably improved antioxidant capacity, demonstrating more than onefold higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging rates compared to parent strain. This strain also exhibited superior LA production in batch fermentation and reached 89.4gL(-1) of LA, with a yield of 0.89gg(-1), a productivity of 1.49gL(-1)h(-1), and an optical purity greate...r than 99%. Furthermore, in fed-batch mode L. paracasei A-22 resulted in 59% higher LA concentration (169.9gL(-1)) compared to parent strain (107.1gL(-1)). The strain adaptation to molasses environment, performed in this study, is a rather simple and promising method for enhancement of LA production on the complex agro-industrial substrate.
Кључне речи:
Strain adaptation / Antioxidant activity / Lactic acid / Fed-batch fermentation / Sugar beet molasses / Potato stillageИзвор:
Applied Biochemistry and Biotechnology, 2019, 187, 3, 753-769Издавач:
- Springer, New York
Финансирање / пројекти:
- Производња млечне киселине и пробиотика на отпадним производима прехрамбене и агро-индустрије (RS-MESTD-Technological Development (TD or TR)-31017)
DOI: 10.1007/s12010-018-2852-x
ISSN: 0273-2289
PubMed: 30058018
WoS: 000463838900007
Scopus: 2-s2.0-85050955692
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Mladenović, Dragana AU - Pejin, Jelena AU - Kocić-Tanackov, Sunčica AU - Đukić-Vuković, Aleksandra AU - Mojović, Ljiljana PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4224 AB - The aim of this study was to perform the adaptation of Lactobacillus paracasei NRRL B-4564 to substrate through adaptive evolution in order to ensure intensive substrate utilization and enhanced L (+)-lactic acid (LA) production on molasses-enriched potato stillage. To evaluate the strain response to environmental conditions exposed during the adaptation process and to select the best adapted cells, the antioxidant activity and LA-producing capability were assessed in batch fermentation. The most promising adapted strain was further used in a pulsed fed-batch mode. Among three selected adapted strains, L. paracasei A-22 showed considerably improved antioxidant capacity, demonstrating more than onefold higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging rates compared to parent strain. This strain also exhibited superior LA production in batch fermentation and reached 89.4gL(-1) of LA, with a yield of 0.89gg(-1), a productivity of 1.49gL(-1)h(-1), and an optical purity greater than 99%. Furthermore, in fed-batch mode L. paracasei A-22 resulted in 59% higher LA concentration (169.9gL(-1)) compared to parent strain (107.1gL(-1)). The strain adaptation to molasses environment, performed in this study, is a rather simple and promising method for enhancement of LA production on the complex agro-industrial substrate. PB - Springer, New York T2 - Applied Biochemistry and Biotechnology T1 - Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate EP - 769 IS - 3 SP - 753 VL - 187 DO - 10.1007/s12010-018-2852-x ER -
@article{ author = "Mladenović, Dragana and Pejin, Jelena and Kocić-Tanackov, Sunčica and Đukić-Vuković, Aleksandra and Mojović, Ljiljana", year = "2019", abstract = "The aim of this study was to perform the adaptation of Lactobacillus paracasei NRRL B-4564 to substrate through adaptive evolution in order to ensure intensive substrate utilization and enhanced L (+)-lactic acid (LA) production on molasses-enriched potato stillage. To evaluate the strain response to environmental conditions exposed during the adaptation process and to select the best adapted cells, the antioxidant activity and LA-producing capability were assessed in batch fermentation. The most promising adapted strain was further used in a pulsed fed-batch mode. Among three selected adapted strains, L. paracasei A-22 showed considerably improved antioxidant capacity, demonstrating more than onefold higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging rates compared to parent strain. This strain also exhibited superior LA production in batch fermentation and reached 89.4gL(-1) of LA, with a yield of 0.89gg(-1), a productivity of 1.49gL(-1)h(-1), and an optical purity greater than 99%. Furthermore, in fed-batch mode L. paracasei A-22 resulted in 59% higher LA concentration (169.9gL(-1)) compared to parent strain (107.1gL(-1)). The strain adaptation to molasses environment, performed in this study, is a rather simple and promising method for enhancement of LA production on the complex agro-industrial substrate.", publisher = "Springer, New York", journal = "Applied Biochemistry and Biotechnology", title = "Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate", pages = "769-753", number = "3", volume = "187", doi = "10.1007/s12010-018-2852-x" }
Mladenović, D., Pejin, J., Kocić-Tanackov, S., Đukić-Vuković, A.,& Mojović, L.. (2019). Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate. in Applied Biochemistry and Biotechnology Springer, New York., 187(3), 753-769. https://doi.org/10.1007/s12010-018-2852-x
Mladenović D, Pejin J, Kocić-Tanackov S, Đukić-Vuković A, Mojović L. Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate. in Applied Biochemistry and Biotechnology. 2019;187(3):753-769. doi:10.1007/s12010-018-2852-x .
Mladenović, Dragana, Pejin, Jelena, Kocić-Tanackov, Sunčica, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, "Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate" in Applied Biochemistry and Biotechnology, 187, no. 3 (2019):753-769, https://doi.org/10.1007/s12010-018-2852-x . .