The aim of this study was to perform the adaptation of Lactobacillus paracasei NRRL B-4564 to substrate through adaptive evolution in order to ensure intensive substrate utilization and enhanced L (+)-lactic acid (LA) production on molasses-enriched potato stillage. To evaluate the strain response to environmental conditions exposed during the adaptation process and to select the best adapted cells, the antioxidant activity and LA-producing capability were assessed in batch fermentation. The most promising adapted strain was further used in a pulsed fed-batch mode. Among three selected adapted strains, L. paracasei A-22 showed considerably improved antioxidant capacity, demonstrating more than onefold higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging rates compared to parent strain. This strain also exhibited superior LA production in batch fermentation and reached 89.4gL(-1) of LA, with a yield of 0.89gg(-1), a productivity of 1.49gL(-1)h(-1), and an optical purity greate...r than 99%. Furthermore, in fed-batch mode L. paracasei A-22 resulted in 59% higher LA concentration (169.9gL(-1)) compared to parent strain (107.1gL(-1)). The strain adaptation to molasses environment, performed in this study, is a rather simple and promising method for enhancement of LA production on the complex agro-industrial substrate.
TY - JOUR
AU - Mladenović, Dragana
AU - Pejin, Jelena
AU - Kocić-Tanackov, Sunčica
AU - Djukić-Vuković, Aleksandra
AU - Mojović, Ljiljana
PY - 2019
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4224
AB - The aim of this study was to perform the adaptation of Lactobacillus paracasei NRRL B-4564 to substrate through adaptive evolution in order to ensure intensive substrate utilization and enhanced L (+)-lactic acid (LA) production on molasses-enriched potato stillage. To evaluate the strain response to environmental conditions exposed during the adaptation process and to select the best adapted cells, the antioxidant activity and LA-producing capability were assessed in batch fermentation. The most promising adapted strain was further used in a pulsed fed-batch mode. Among three selected adapted strains, L. paracasei A-22 showed considerably improved antioxidant capacity, demonstrating more than onefold higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging rates compared to parent strain. This strain also exhibited superior LA production in batch fermentation and reached 89.4gL(-1) of LA, with a yield of 0.89gg(-1), a productivity of 1.49gL(-1)h(-1), and an optical purity greater than 99%. Furthermore, in fed-batch mode L. paracasei A-22 resulted in 59% higher LA concentration (169.9gL(-1)) compared to parent strain (107.1gL(-1)). The strain adaptation to molasses environment, performed in this study, is a rather simple and promising method for enhancement of LA production on the complex agro-industrial substrate.
PB - Springer, New York
T2 - Applied Biochemistry and Biotechnology
T1 - Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate
EP - 769
IS - 3
SP - 753
VL - 187
DO - 10.1007/s12010-018-2852-x
ER -
@article{
author = "Mladenović, Dragana and Pejin, Jelena and Kocić-Tanackov, Sunčica and Djukić-Vuković, Aleksandra and Mojović, Ljiljana",
year = "2019",
abstract = "The aim of this study was to perform the adaptation of Lactobacillus paracasei NRRL B-4564 to substrate through adaptive evolution in order to ensure intensive substrate utilization and enhanced L (+)-lactic acid (LA) production on molasses-enriched potato stillage. To evaluate the strain response to environmental conditions exposed during the adaptation process and to select the best adapted cells, the antioxidant activity and LA-producing capability were assessed in batch fermentation. The most promising adapted strain was further used in a pulsed fed-batch mode. Among three selected adapted strains, L. paracasei A-22 showed considerably improved antioxidant capacity, demonstrating more than onefold higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging rates compared to parent strain. This strain also exhibited superior LA production in batch fermentation and reached 89.4gL(-1) of LA, with a yield of 0.89gg(-1), a productivity of 1.49gL(-1)h(-1), and an optical purity greater than 99%. Furthermore, in fed-batch mode L. paracasei A-22 resulted in 59% higher LA concentration (169.9gL(-1)) compared to parent strain (107.1gL(-1)). The strain adaptation to molasses environment, performed in this study, is a rather simple and promising method for enhancement of LA production on the complex agro-industrial substrate.",
publisher = "Springer, New York",
journal = "Applied Biochemistry and Biotechnology",
title = "Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate",
pages = "769-753",
number = "3",
volume = "187",
doi = "10.1007/s12010-018-2852-x"
}
Mladenović, D., Pejin, J., Kocić-Tanackov, S., Djukić-Vuković, A.,& Mojović, L.. (2019). Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate. in Applied Biochemistry and Biotechnology
Springer, New York., 187(3), 753-769.
https://doi.org/10.1007/s12010-018-2852-x
Mladenović D, Pejin J, Kocić-Tanackov S, Djukić-Vuković A, Mojović L. Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate. in Applied Biochemistry and Biotechnology. 2019;187(3):753-769.
doi:10.1007/s12010-018-2852-x .
Mladenović, Dragana, Pejin, Jelena, Kocić-Tanackov, Sunčica, Djukić-Vuković, Aleksandra, Mojović, Ljiljana, "Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate" in Applied Biochemistry and Biotechnology, 187, no. 3 (2019):753-769,
https://doi.org/10.1007/s12010-018-2852-x . .
