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Electrochemical behavior of esomeprazole: Its determination at Au electrode as standard and in injection powder combined with the study of its degradation
Esomeprazole is the most effective of the proton-pump inhibitors for the acid-related diseases and at first was examined for the electroanalytical purposes. The drug standard and as a content of injection powder was investigated by cyclic voltammetry (CV) and quantitatively determined using square wave voltammetry (SWV) via its electrooxidation at Au electrode in 0.05 M NaHCO3. SWV showed a linear dependency of the anodic peak currents vs. esomeprazole standard concentrations in the range from 3.0 to 500 mu g mL(-1) with the values of limit of detection (LOD) and limit of quantification (LOQ): 1.4 and 4.6 mu g mL(-1), respectively. Using the constructed and validated calibration curve, the values of unknown esomeprazole concentrations in injection powder and in human serum spiked with standard were determined. Before the electrochemical oxidation, it was shown by atomic force microscopy (AFM) that the small esomeprazole islands formed inside holes were visible and their diameter was ab...out 200 nm attributed to physico-chemical characteristics of esomeprazole. After the electrochemical oxidation, the morphology of esomeprazole standard on Au surface was completely changed and composed of spherical particles in a diameter between 200 and 600 nm. With esomeprazole suspended in human serum, the process of crystallization partly occurred in the form of spherical grains with the average size of these grains was about 4 gm. The analysis at the macro level done by the optical microscopy (OM) confirmed this opinion. The study of esomeprazole degradation showed that at Au electrode, after 3 h of cycling, a neglectable amount of the esomeprazole was changed. Using IrOx electrode under directed stress conditions, its almost complete degradation was realized after 3 h confirmed by high performance liquid chromatography (HPLC). Total organic carbon (TOC) analysis showed that 95% of esomeprazole was mineralized. The HPLC and Liquid chromatography-mass spectrometry (LC-MS) study revealed the formation of 4-hydroxy omeprazole sulphide, 4-hydroxy omeprazole sulphone, esomeprazole sulphone and methylated esomeprazole.
Keywords:
Esomeprazole / SWV determination at Au / Au surface analysis by AFM and OM / Degradation at IrOx / HPLC / LC-MS
TY - JOUR
AU - Avramov-Ivić, Milka
AU - Lović, Jelena
AU - Stevanović, Sanja
AU - Nikolić, Nebojša
AU - Trišović, Nemanja
AU - Lađarević, Jelena
AU - Vuković, Dragan
AU - Drmanić, Saša
AU - Mladenović, Aleksandar R.
AU - Jadranin, Milka
AU - Petrović, Slobodan
AU - Mijin, Dušan
PY - 2019
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4306
AB - Esomeprazole is the most effective of the proton-pump inhibitors for the acid-related diseases and at first was examined for the electroanalytical purposes. The drug standard and as a content of injection powder was investigated by cyclic voltammetry (CV) and quantitatively determined using square wave voltammetry (SWV) via its electrooxidation at Au electrode in 0.05 M NaHCO3. SWV showed a linear dependency of the anodic peak currents vs. esomeprazole standard concentrations in the range from 3.0 to 500 mu g mL(-1) with the values of limit of detection (LOD) and limit of quantification (LOQ): 1.4 and 4.6 mu g mL(-1), respectively. Using the constructed and validated calibration curve, the values of unknown esomeprazole concentrations in injection powder and in human serum spiked with standard were determined. Before the electrochemical oxidation, it was shown by atomic force microscopy (AFM) that the small esomeprazole islands formed inside holes were visible and their diameter was about 200 nm attributed to physico-chemical characteristics of esomeprazole. After the electrochemical oxidation, the morphology of esomeprazole standard on Au surface was completely changed and composed of spherical particles in a diameter between 200 and 600 nm. With esomeprazole suspended in human serum, the process of crystallization partly occurred in the form of spherical grains with the average size of these grains was about 4 gm. The analysis at the macro level done by the optical microscopy (OM) confirmed this opinion. The study of esomeprazole degradation showed that at Au electrode, after 3 h of cycling, a neglectable amount of the esomeprazole was changed. Using IrOx electrode under directed stress conditions, its almost complete degradation was realized after 3 h confirmed by high performance liquid chromatography (HPLC). Total organic carbon (TOC) analysis showed that 95% of esomeprazole was mineralized. The HPLC and Liquid chromatography-mass spectrometry (LC-MS) study revealed the formation of 4-hydroxy omeprazole sulphide, 4-hydroxy omeprazole sulphone, esomeprazole sulphone and methylated esomeprazole.
PB - Elsevier Science Sa, Lausanne
T2 - Journal of Electroanalytical Chemistry
T1 - Electrochemical behavior of esomeprazole: Its determination at Au electrode as standard and in injection powder combined with the study of its degradation
VL - 848
DO - 10.1016/j.jelechem.2019.113303
ER -
@article{
author = "Avramov-Ivić, Milka and Lović, Jelena and Stevanović, Sanja and Nikolić, Nebojša and Trišović, Nemanja and Lađarević, Jelena and Vuković, Dragan and Drmanić, Saša and Mladenović, Aleksandar R. and Jadranin, Milka and Petrović, Slobodan and Mijin, Dušan",
year = "2019",
abstract = "Esomeprazole is the most effective of the proton-pump inhibitors for the acid-related diseases and at first was examined for the electroanalytical purposes. The drug standard and as a content of injection powder was investigated by cyclic voltammetry (CV) and quantitatively determined using square wave voltammetry (SWV) via its electrooxidation at Au electrode in 0.05 M NaHCO3. SWV showed a linear dependency of the anodic peak currents vs. esomeprazole standard concentrations in the range from 3.0 to 500 mu g mL(-1) with the values of limit of detection (LOD) and limit of quantification (LOQ): 1.4 and 4.6 mu g mL(-1), respectively. Using the constructed and validated calibration curve, the values of unknown esomeprazole concentrations in injection powder and in human serum spiked with standard were determined. Before the electrochemical oxidation, it was shown by atomic force microscopy (AFM) that the small esomeprazole islands formed inside holes were visible and their diameter was about 200 nm attributed to physico-chemical characteristics of esomeprazole. After the electrochemical oxidation, the morphology of esomeprazole standard on Au surface was completely changed and composed of spherical particles in a diameter between 200 and 600 nm. With esomeprazole suspended in human serum, the process of crystallization partly occurred in the form of spherical grains with the average size of these grains was about 4 gm. The analysis at the macro level done by the optical microscopy (OM) confirmed this opinion. The study of esomeprazole degradation showed that at Au electrode, after 3 h of cycling, a neglectable amount of the esomeprazole was changed. Using IrOx electrode under directed stress conditions, its almost complete degradation was realized after 3 h confirmed by high performance liquid chromatography (HPLC). Total organic carbon (TOC) analysis showed that 95% of esomeprazole was mineralized. The HPLC and Liquid chromatography-mass spectrometry (LC-MS) study revealed the formation of 4-hydroxy omeprazole sulphide, 4-hydroxy omeprazole sulphone, esomeprazole sulphone and methylated esomeprazole.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Electroanalytical Chemistry",
title = "Electrochemical behavior of esomeprazole: Its determination at Au electrode as standard and in injection powder combined with the study of its degradation",
volume = "848",
doi = "10.1016/j.jelechem.2019.113303"
}
Avramov-Ivić, M., Lović, J., Stevanović, S., Nikolić, N., Trišović, N., Lađarević, J., Vuković, D., Drmanić, S., Mladenović, A. R., Jadranin, M., Petrović, S.,& Mijin, D.. (2019). Electrochemical behavior of esomeprazole: Its determination at Au electrode as standard and in injection powder combined with the study of its degradation. in Journal of Electroanalytical Chemistry
Elsevier Science Sa, Lausanne., 848.
https://doi.org/10.1016/j.jelechem.2019.113303
Avramov-Ivić M, Lović J, Stevanović S, Nikolić N, Trišović N, Lađarević J, Vuković D, Drmanić S, Mladenović AR, Jadranin M, Petrović S, Mijin D. Electrochemical behavior of esomeprazole: Its determination at Au electrode as standard and in injection powder combined with the study of its degradation. in Journal of Electroanalytical Chemistry. 2019;848.
doi:10.1016/j.jelechem.2019.113303 .
Avramov-Ivić, Milka, Lović, Jelena, Stevanović, Sanja, Nikolić, Nebojša, Trišović, Nemanja, Lađarević, Jelena, Vuković, Dragan, Drmanić, Saša, Mladenović, Aleksandar R., Jadranin, Milka, Petrović, Slobodan, Mijin, Dušan, "Electrochemical behavior of esomeprazole: Its determination at Au electrode as standard and in injection powder combined with the study of its degradation" in Journal of Electroanalytical Chemistry, 848 (2019),
https://doi.org/10.1016/j.jelechem.2019.113303 . .
