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dc.creatorStojkovska, Jasmina
dc.creatorZvicer, Jovana
dc.creatorMilivojević, Milena
dc.creatorPetrović, Isidora
dc.creatorStevanović, Milena
dc.creatorObradović, Bojana
dc.date.accessioned2021-03-10T14:14:46Z
dc.date.available2021-03-10T14:14:46Z
dc.date.issued2020
dc.identifier.issn0367-598X
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/4373
dc.description.abstractDevelopment of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.en
dc.publisherSavez hemijskih inženjera, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200042/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceHemijska industrija
dc.subject3D cultureen
dc.subjectbiomimetic bioreactoren
dc.subjecttumor modelen
dc.subjectalginate microfibersen
dc.titleValidation of a novel perfusion bioreactor system in cancer researchen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage196
dc.citation.issue3
dc.citation.other74(3): 187-196
dc.citation.rankM23~
dc.citation.spage187
dc.citation.volume74
dc.identifier.doi10.2298/HEMIND200329015S
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/8307/bitstream_8307.pdf
dc.identifier.rcubconv_6226
dc.identifier.scopus2-s2.0-85087435489
dc.identifier.wos000577922500003
dc.type.versionpublishedVersion


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