Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes

2020
Authors
Bebić, JelenaBanjanac, Katarina

Ćorović, Marija

Milivojević, Ana

Simović, Milica

Vukoičić, Ana Ž.

Mitrović, Danica D.
Bezbradica, Dejan

Article (Published version)
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In this study, immobilization of laccase from Trametes versicolor on eight Lifetech (TM) supports, with different characteristics (pore size, length of the spacer arm and functional groups), was studied and optimized for intended use in bioremediation for decolorization of industrial wastewaters. Out of six tested amino-functionalized supports, the most promising carrier was proved to be porous Lifetech (TM) ECR8309F with primary amino groups and a C2 spacer arm. Onto this support, laccase is attached by forming electrostatic interactions so that the most active preparation has shown the activity of 66876 U/g support. On the other hand, during immobilization of laccase on epoxy-functionalized Lifetech (TM) ECR8285F, via hydrophobic interactions and covalent bonding confirmed by a desorption assay, immobilization yield of 60 % and the activity of 118929 U/g were accomplished. Furthermore, immobilized enzyme on this support showed high capacity for decolorization of dyes (Lanaset (R) Vio...let B, Lanaset (R) Blue 2R, bromothymol blue and bromocresol green), by combination of both adsorption and enzyme degradation. Decolorization was in the range of 88 to 96 % after 4 h, with more than 80 % achieved after only 45 min. Also, this preparation demonstrated high operational stability during seven consecutive reuses in all examined dye reaction systems.
Keywords:
enzyme immobilization / bioremediation / textile dyes / decolorization / wastewater treatmentSource:
Hemijska industrija, 2020, 74, 3, 197-209Publisher:
- Savez hemijskih inženjera, Beograd
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-200135)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-200287)
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Tehnološko-metalurški fakultetTY - JOUR AU - Bebić, Jelena AU - Banjanac, Katarina AU - Ćorović, Marija AU - Milivojević, Ana AU - Simović, Milica AU - Vukoičić, Ana Ž. AU - Mitrović, Danica D. AU - Bezbradica, Dejan PY - 2020 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4374 AB - In this study, immobilization of laccase from Trametes versicolor on eight Lifetech (TM) supports, with different characteristics (pore size, length of the spacer arm and functional groups), was studied and optimized for intended use in bioremediation for decolorization of industrial wastewaters. Out of six tested amino-functionalized supports, the most promising carrier was proved to be porous Lifetech (TM) ECR8309F with primary amino groups and a C2 spacer arm. Onto this support, laccase is attached by forming electrostatic interactions so that the most active preparation has shown the activity of 66876 U/g support. On the other hand, during immobilization of laccase on epoxy-functionalized Lifetech (TM) ECR8285F, via hydrophobic interactions and covalent bonding confirmed by a desorption assay, immobilization yield of 60 % and the activity of 118929 U/g were accomplished. Furthermore, immobilized enzyme on this support showed high capacity for decolorization of dyes (Lanaset (R) Violet B, Lanaset (R) Blue 2R, bromothymol blue and bromocresol green), by combination of both adsorption and enzyme degradation. Decolorization was in the range of 88 to 96 % after 4 h, with more than 80 % achieved after only 45 min. Also, this preparation demonstrated high operational stability during seven consecutive reuses in all examined dye reaction systems. PB - Savez hemijskih inženjera, Beograd T2 - Hemijska industrija T1 - Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes EP - 209 IS - 3 SP - 197 VL - 74 DO - 10.2298/HEMIND200320016B ER -
@article{ author = "Bebić, Jelena and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Vukoičić, Ana Ž. and Mitrović, Danica D. and Bezbradica, Dejan", year = "2020", abstract = "In this study, immobilization of laccase from Trametes versicolor on eight Lifetech (TM) supports, with different characteristics (pore size, length of the spacer arm and functional groups), was studied and optimized for intended use in bioremediation for decolorization of industrial wastewaters. Out of six tested amino-functionalized supports, the most promising carrier was proved to be porous Lifetech (TM) ECR8309F with primary amino groups and a C2 spacer arm. Onto this support, laccase is attached by forming electrostatic interactions so that the most active preparation has shown the activity of 66876 U/g support. On the other hand, during immobilization of laccase on epoxy-functionalized Lifetech (TM) ECR8285F, via hydrophobic interactions and covalent bonding confirmed by a desorption assay, immobilization yield of 60 % and the activity of 118929 U/g were accomplished. Furthermore, immobilized enzyme on this support showed high capacity for decolorization of dyes (Lanaset (R) Violet B, Lanaset (R) Blue 2R, bromothymol blue and bromocresol green), by combination of both adsorption and enzyme degradation. Decolorization was in the range of 88 to 96 % after 4 h, with more than 80 % achieved after only 45 min. Also, this preparation demonstrated high operational stability during seven consecutive reuses in all examined dye reaction systems.", publisher = "Savez hemijskih inženjera, Beograd", journal = "Hemijska industrija", title = "Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes", pages = "209-197", number = "3", volume = "74", doi = "10.2298/HEMIND200320016B" }
Bebić, J., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Vukoičić, A. Ž., Mitrović, D. D.,& Bezbradica, D.. (2020). Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes. in Hemijska industrija Savez hemijskih inženjera, Beograd., 74(3), 197-209. https://doi.org/10.2298/HEMIND200320016B
Bebić J, Banjanac K, Ćorović M, Milivojević A, Simović M, Vukoičić AŽ, Mitrović DD, Bezbradica D. Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes. in Hemijska industrija. 2020;74(3):197-209. doi:10.2298/HEMIND200320016B .
Bebić, Jelena, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Vukoičić, Ana Ž., Mitrović, Danica D., Bezbradica, Dejan, "Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes" in Hemijska industrija, 74, no. 3 (2020):197-209, https://doi.org/10.2298/HEMIND200320016B . .