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Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

Authorized Users Only
2020
Authors
Bebić, Jelena
Banjanac, Katarina
Ćorović, Marija
Milivojević, Ana
Simović, Milica
Marinković, Aleksandar
Bezbradica, Dejan
Article (Published version)
Metadata
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Abstract
This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was ...reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.

Keywords:
Silica nanoparticles / Laccase / Enzyme immobilization / Lindane / Bioremediation
Source:
Chinese Journal of Chemical Engineering, 2020, 28, 4, 1136-1144
Publisher:
  • Chemical Industry Press Co Ltd, Beijing
Funding / projects:
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
  • Synthesis, processing and applications of nanostructured multifunctional materials with defined properties (RS-45019)
  • Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-31035)

DOI: 10.1016/j.cjche.2019.12.025

ISSN: 1004-9541

WoS: 000555483300022

Scopus: 2-s2.0-85081279850
[ Google Scholar ]
23
9
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4384
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
  • Radovi istraživača (Inovacioni centar) / Researchers’ publications (Innovation Centre)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Bebić, Jelena
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Marinković, Aleksandar
AU  - Bezbradica, Dejan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4384
AB  - This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.
PB  - Chemical Industry Press Co Ltd, Beijing
T2  - Chinese Journal of Chemical Engineering
T1  - Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation
EP  - 1144
IS  - 4
SP  - 1136
VL  - 28
DO  - 10.1016/j.cjche.2019.12.025
ER  - 
@article{
author = "Bebić, Jelena and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Marinković, Aleksandar and Bezbradica, Dejan",
year = "2020",
abstract = "This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.",
publisher = "Chemical Industry Press Co Ltd, Beijing",
journal = "Chinese Journal of Chemical Engineering",
title = "Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation",
pages = "1144-1136",
number = "4",
volume = "28",
doi = "10.1016/j.cjche.2019.12.025"
}
Bebić, J., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Marinković, A.,& Bezbradica, D.. (2020). Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation. in Chinese Journal of Chemical Engineering
Chemical Industry Press Co Ltd, Beijing., 28(4), 1136-1144.
https://doi.org/10.1016/j.cjche.2019.12.025
Bebić J, Banjanac K, Ćorović M, Milivojević A, Simović M, Marinković A, Bezbradica D. Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation. in Chinese Journal of Chemical Engineering. 2020;28(4):1136-1144.
doi:10.1016/j.cjche.2019.12.025 .
Bebić, Jelena, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Marinković, Aleksandar, Bezbradica, Dejan, "Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation" in Chinese Journal of Chemical Engineering, 28, no. 4 (2020):1136-1144,
https://doi.org/10.1016/j.cjche.2019.12.025 . .

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