Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation
Само за регистроване кориснике
2020
Аутори
Bebić, JelenaBanjanac, Katarina
Ćorović, Marija
Milivojević, Ana
Simović, Milica
Marinković, Aleksandar
Bezbradica, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was ...reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.
Кључне речи:
Silica nanoparticles / Laccase / Enzyme immobilization / Lindane / BioremediationИзвор:
Chinese Journal of Chemical Engineering, 2020, 28, 4, 1136-1144Издавач:
- Chemical Industry Press Co Ltd, Beijing
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-46010)
- Синтеза, развој технологија добијања и примена наноструктурних мултифункционалних материјала дефинисаних својстава (RS-45019)
- Примена биотехнолошких метода у одрживом искоришћењу нус-производа агроиндустрије (RS-31035)
DOI: 10.1016/j.cjche.2019.12.025
ISSN: 1004-9541
WoS: 000555483300022
Scopus: 2-s2.0-85081279850
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Bebić, Jelena AU - Banjanac, Katarina AU - Ćorović, Marija AU - Milivojević, Ana AU - Simović, Milica AU - Marinković, Aleksandar AU - Bezbradica, Dejan PY - 2020 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4384 AB - This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity. PB - Chemical Industry Press Co Ltd, Beijing T2 - Chinese Journal of Chemical Engineering T1 - Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation EP - 1144 IS - 4 SP - 1136 VL - 28 DO - 10.1016/j.cjche.2019.12.025 ER -
@article{ author = "Bebić, Jelena and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Marinković, Aleksandar and Bezbradica, Dejan", year = "2020", abstract = "This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.", publisher = "Chemical Industry Press Co Ltd, Beijing", journal = "Chinese Journal of Chemical Engineering", title = "Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation", pages = "1144-1136", number = "4", volume = "28", doi = "10.1016/j.cjche.2019.12.025" }
Bebić, J., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Marinković, A.,& Bezbradica, D.. (2020). Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation. in Chinese Journal of Chemical Engineering Chemical Industry Press Co Ltd, Beijing., 28(4), 1136-1144. https://doi.org/10.1016/j.cjche.2019.12.025
Bebić J, Banjanac K, Ćorović M, Milivojević A, Simović M, Marinković A, Bezbradica D. Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation. in Chinese Journal of Chemical Engineering. 2020;28(4):1136-1144. doi:10.1016/j.cjche.2019.12.025 .
Bebić, Jelena, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Marinković, Aleksandar, Bezbradica, Dejan, "Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation" in Chinese Journal of Chemical Engineering, 28, no. 4 (2020):1136-1144, https://doi.org/10.1016/j.cjche.2019.12.025 . .