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The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms

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Authors
Klaus, Anita
Petrović, Predrag
Vunduk, Jovana
Pavlović, Vladimir
van Griensven, Leo
Article (Published version)
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Abstract
Crude polysaccharide extracts derived from Agaricus bisporus (A), A. brasiliensis (B), and Phellinus linteus (P) were used for the synthesis of silver nanoparticles (AgNPs, labeled AAgNP, BAgNP, PAgNP, respectively) UV and FT-IR spectra, size distribution, and zeta potential of the respective particles were recorded, and TEM and SEM-EDS characterization was done. UV-Vis spectroscopy showed maximum absorbance at 415 mu for A, at 400 nm for P, and at 420 nm for B. SEM-EDS analysis showed BAgNP to consist of almost 90% of silver, while silver content in AAgNP and PAgNP was much lower, 46.5 and 52.3%, respectively. Individual extracts as well as the Ag-NPs were tested against a panel of clinically isolated and ATCC derived pathogens. In almost all cases minimum inhibitory concentration (MIC) was significantly lower than those of antibiotics, suggesting up to 100 times more effectivity. BAgNP, PAgNP, and AAgNP appeared very active against Pseudomonas aeruginosa ATCC 27853 (MIC: 0.19, 0.19, ...0.97 mu g/mL, respectively). The same nanoparticles were also very effective toward Candida albicans ATCC 10231 (MIC: 0.39, 0.78, 0.97 mu g/mL, respectively). Our study indicates that the bactericidal effect of PAgNP, AAgNP, BAgNP on E. coli 25922 ATCC (MBC: 1.56, 0.97, 6.25 mu g/mL, respectively) is significantly more pronounced than that of amoxicillin. A pure solution of colloidal silver nanoparticles showed significantly weaker microbiostatic/microbiocidal potential than all tested mushroom extracts' AgNPs, as well as amoxicillin. Silver nanoparticles made with extracts of A, B, and P seem welcome as an addition to the inventory of antimicrobial compounds used in clinical medicine against bacterial and yeast infection.

Keywords:
Agaricus bisporus / A. brasiliensis / Phellinus linteus / mushroom polysaccharide extracts / silver nanoparticles / antibacterial activity / antifungal activity / medicinal mushrooms
Source:
International Journal of Medicinal Mushrooms, 2020, 22, 9, 869-883
Publisher:
  • Begell House Inc, Danbury
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200116 (University of Belgrade, Faculty of Agriculture) (RS-200116)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200116 (University of Belgrade, Faculty of Agriculture) (RS-200116)

DOI: 10.1615/IntJMedMushrooms.2020035988

ISSN: 1521-9437

WoS: 000579434700005

Scopus: 2-s2.0-85092259078
[ Google Scholar ]
16
3
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4414
Collections
  • Radovi istraživača (Inovacioni centar) / Researchers’ publications (Innovation Centre)
Institution/Community
Inovacioni centar
TY  - JOUR
AU  - Klaus, Anita
AU  - Petrović, Predrag
AU  - Vunduk, Jovana
AU  - Pavlović, Vladimir
AU  - van Griensven, Leo
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4414
AB  - Crude polysaccharide extracts derived from Agaricus bisporus (A), A. brasiliensis (B), and Phellinus linteus (P) were used for the synthesis of silver nanoparticles (AgNPs, labeled AAgNP, BAgNP, PAgNP, respectively) UV and FT-IR spectra, size distribution, and zeta potential of the respective particles were recorded, and TEM and SEM-EDS characterization was done. UV-Vis spectroscopy showed maximum absorbance at 415 mu for A, at 400 nm for P, and at 420 nm for B. SEM-EDS analysis showed BAgNP to consist of almost 90% of silver, while silver content in AAgNP and PAgNP was much lower, 46.5 and 52.3%, respectively. Individual extracts as well as the Ag-NPs were tested against a panel of clinically isolated and ATCC derived pathogens. In almost all cases minimum inhibitory concentration (MIC) was significantly lower than those of antibiotics, suggesting up to 100 times more effectivity. BAgNP, PAgNP, and AAgNP appeared very active against Pseudomonas aeruginosa ATCC 27853 (MIC: 0.19, 0.19, 0.97 mu g/mL, respectively). The same nanoparticles were also very effective toward Candida albicans ATCC 10231 (MIC: 0.39, 0.78, 0.97 mu g/mL, respectively). Our study indicates that the bactericidal effect of PAgNP, AAgNP, BAgNP on E. coli 25922 ATCC (MBC: 1.56, 0.97, 6.25 mu g/mL, respectively) is significantly more pronounced than that of amoxicillin. A pure solution of colloidal silver nanoparticles showed significantly weaker microbiostatic/microbiocidal potential than all tested mushroom extracts' AgNPs, as well as amoxicillin. Silver nanoparticles made with extracts of A, B, and P seem welcome as an addition to the inventory of antimicrobial compounds used in clinical medicine against bacterial and yeast infection.
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms
EP  - 883
IS  - 9
SP  - 869
VL  - 22
DO  - 10.1615/IntJMedMushrooms.2020035988
ER  - 
@article{
author = "Klaus, Anita and Petrović, Predrag and Vunduk, Jovana and Pavlović, Vladimir and van Griensven, Leo",
year = "2020",
abstract = "Crude polysaccharide extracts derived from Agaricus bisporus (A), A. brasiliensis (B), and Phellinus linteus (P) were used for the synthesis of silver nanoparticles (AgNPs, labeled AAgNP, BAgNP, PAgNP, respectively) UV and FT-IR spectra, size distribution, and zeta potential of the respective particles were recorded, and TEM and SEM-EDS characterization was done. UV-Vis spectroscopy showed maximum absorbance at 415 mu for A, at 400 nm for P, and at 420 nm for B. SEM-EDS analysis showed BAgNP to consist of almost 90% of silver, while silver content in AAgNP and PAgNP was much lower, 46.5 and 52.3%, respectively. Individual extracts as well as the Ag-NPs were tested against a panel of clinically isolated and ATCC derived pathogens. In almost all cases minimum inhibitory concentration (MIC) was significantly lower than those of antibiotics, suggesting up to 100 times more effectivity. BAgNP, PAgNP, and AAgNP appeared very active against Pseudomonas aeruginosa ATCC 27853 (MIC: 0.19, 0.19, 0.97 mu g/mL, respectively). The same nanoparticles were also very effective toward Candida albicans ATCC 10231 (MIC: 0.39, 0.78, 0.97 mu g/mL, respectively). Our study indicates that the bactericidal effect of PAgNP, AAgNP, BAgNP on E. coli 25922 ATCC (MBC: 1.56, 0.97, 6.25 mu g/mL, respectively) is significantly more pronounced than that of amoxicillin. A pure solution of colloidal silver nanoparticles showed significantly weaker microbiostatic/microbiocidal potential than all tested mushroom extracts' AgNPs, as well as amoxicillin. Silver nanoparticles made with extracts of A, B, and P seem welcome as an addition to the inventory of antimicrobial compounds used in clinical medicine against bacterial and yeast infection.",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms",
pages = "883-869",
number = "9",
volume = "22",
doi = "10.1615/IntJMedMushrooms.2020035988"
}
Klaus, A., Petrović, P., Vunduk, J., Pavlović, V.,& van Griensven, L.. (2020). The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms. in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 22(9), 869-883.
https://doi.org/10.1615/IntJMedMushrooms.2020035988
Klaus A, Petrović P, Vunduk J, Pavlović V, van Griensven L. The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms. in International Journal of Medicinal Mushrooms. 2020;22(9):869-883.
doi:10.1615/IntJMedMushrooms.2020035988 .
Klaus, Anita, Petrović, Predrag, Vunduk, Jovana, Pavlović, Vladimir, van Griensven, Leo, "The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms" in International Journal of Medicinal Mushrooms, 22, no. 9 (2020):869-883,
https://doi.org/10.1615/IntJMedMushrooms.2020035988 . .

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