Amino-modified kraft lignin microspheres as a support for enzyme immobilization
Autori
Bebić, JelenaBanjanac, Katarina
Rusmirović, Jelena
Ćorović, Marija
Milivojević, Ana
Simović, Milica
Marinković, Aleksandar
Bezbradica, Dejan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this research, it has been demonstrated that amino-modified microspheres (A-LMS) based on bio-waste derived material, such as kraft lignin, have good prospects in usage as a support for enzyme immobilization, since active biocatalyst systems were prepared by immobilizing beta-galactosidase fromA. oryzaeand laccase fromM. thermophilaexpressed inA. oryzae(Novozym (R) 51003) onto A-LMS. Two types of A-LMS were investigated, with different emulsifier concentrations (5 wt% and 10 wt%), and microspheres produced using 5 wt% of emulsifier (A-LMS_5) showed adequate pore shape, size and distribution for enzyme attachment. The type of interactions formed between enzymes (beta-galactosidase and laccase) and A-LMS_5 microspheres demonstrated that beta-galactosidase is predominantly attachedviaelectrostatic interactions while attachment of laccase is equally governed by electrostatic and hydrophobic interactions. Furthermore, the A-LMS_5-beta-galactosidase exhibited specificity towards recognize...d prebiotics (galacto-oligosaccharides (GOS)) synthesis with 1.5-times higher GOS production than glucose production, while for environmental pollutant lindane degradation, the immobilized laccase preparation exhibited high activity with a minimum remaining lindane concentration of 22.4% after 6 days. Thus, this novel enzyme immobilization support A-LMS_5 has potential for use in green biotechnologies.
Izvor:
RSC Advances, 2020, 10, 36, 21495-21508Izdavač:
- Royal Society of Chemistry
Finansiranje / projekti:
- COST (European Cooperation in Science and Technology)European Cooperation in Science and Technology (COST) [CA17128]
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-MESTD-inst-2020-200287)
DOI: 10.1039/d0ra03439h
ISSN: 2046-2069
WoS: 000542722900049
Scopus: 2-s2.0-85086520859
Kolekcije
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Bebić, Jelena AU - Banjanac, Katarina AU - Rusmirović, Jelena AU - Ćorović, Marija AU - Milivojević, Ana AU - Simović, Milica AU - Marinković, Aleksandar AU - Bezbradica, Dejan PY - 2020 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4510 AB - In this research, it has been demonstrated that amino-modified microspheres (A-LMS) based on bio-waste derived material, such as kraft lignin, have good prospects in usage as a support for enzyme immobilization, since active biocatalyst systems were prepared by immobilizing beta-galactosidase fromA. oryzaeand laccase fromM. thermophilaexpressed inA. oryzae(Novozym (R) 51003) onto A-LMS. Two types of A-LMS were investigated, with different emulsifier concentrations (5 wt% and 10 wt%), and microspheres produced using 5 wt% of emulsifier (A-LMS_5) showed adequate pore shape, size and distribution for enzyme attachment. The type of interactions formed between enzymes (beta-galactosidase and laccase) and A-LMS_5 microspheres demonstrated that beta-galactosidase is predominantly attachedviaelectrostatic interactions while attachment of laccase is equally governed by electrostatic and hydrophobic interactions. Furthermore, the A-LMS_5-beta-galactosidase exhibited specificity towards recognized prebiotics (galacto-oligosaccharides (GOS)) synthesis with 1.5-times higher GOS production than glucose production, while for environmental pollutant lindane degradation, the immobilized laccase preparation exhibited high activity with a minimum remaining lindane concentration of 22.4% after 6 days. Thus, this novel enzyme immobilization support A-LMS_5 has potential for use in green biotechnologies. PB - Royal Society of Chemistry T2 - RSC Advances T1 - Amino-modified kraft lignin microspheres as a support for enzyme immobilization EP - 21508 IS - 36 SP - 21495 VL - 10 DO - 10.1039/d0ra03439h ER -
@article{ author = "Bebić, Jelena and Banjanac, Katarina and Rusmirović, Jelena and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Marinković, Aleksandar and Bezbradica, Dejan", year = "2020", abstract = "In this research, it has been demonstrated that amino-modified microspheres (A-LMS) based on bio-waste derived material, such as kraft lignin, have good prospects in usage as a support for enzyme immobilization, since active biocatalyst systems were prepared by immobilizing beta-galactosidase fromA. oryzaeand laccase fromM. thermophilaexpressed inA. oryzae(Novozym (R) 51003) onto A-LMS. Two types of A-LMS were investigated, with different emulsifier concentrations (5 wt% and 10 wt%), and microspheres produced using 5 wt% of emulsifier (A-LMS_5) showed adequate pore shape, size and distribution for enzyme attachment. The type of interactions formed between enzymes (beta-galactosidase and laccase) and A-LMS_5 microspheres demonstrated that beta-galactosidase is predominantly attachedviaelectrostatic interactions while attachment of laccase is equally governed by electrostatic and hydrophobic interactions. Furthermore, the A-LMS_5-beta-galactosidase exhibited specificity towards recognized prebiotics (galacto-oligosaccharides (GOS)) synthesis with 1.5-times higher GOS production than glucose production, while for environmental pollutant lindane degradation, the immobilized laccase preparation exhibited high activity with a minimum remaining lindane concentration of 22.4% after 6 days. Thus, this novel enzyme immobilization support A-LMS_5 has potential for use in green biotechnologies.", publisher = "Royal Society of Chemistry", journal = "RSC Advances", title = "Amino-modified kraft lignin microspheres as a support for enzyme immobilization", pages = "21508-21495", number = "36", volume = "10", doi = "10.1039/d0ra03439h" }
Bebić, J., Banjanac, K., Rusmirović, J., Ćorović, M., Milivojević, A., Simović, M., Marinković, A.,& Bezbradica, D.. (2020). Amino-modified kraft lignin microspheres as a support for enzyme immobilization. in RSC Advances Royal Society of Chemistry., 10(36), 21495-21508. https://doi.org/10.1039/d0ra03439h
Bebić J, Banjanac K, Rusmirović J, Ćorović M, Milivojević A, Simović M, Marinković A, Bezbradica D. Amino-modified kraft lignin microspheres as a support for enzyme immobilization. in RSC Advances. 2020;10(36):21495-21508. doi:10.1039/d0ra03439h .
Bebić, Jelena, Banjanac, Katarina, Rusmirović, Jelena, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Marinković, Aleksandar, Bezbradica, Dejan, "Amino-modified kraft lignin microspheres as a support for enzyme immobilization" in RSC Advances, 10, no. 36 (2020):21495-21508, https://doi.org/10.1039/d0ra03439h . .