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dc.creatorMihajlovski, Katarina
dc.creatorRajilić-Stojanović, Mirjana
dc.creatorDimitrijević-Branković, Suzana
dc.date.accessioned2021-03-10T14:25:09Z
dc.date.available2021-03-10T14:25:09Z
dc.date.issued2020
dc.identifier.issn0960-1481
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/4536
dc.description.abstractMicrobial hydrolytic enzymes are relevant biotechnological products that can be applied in various industries. In this study, we have tested the activity of hydrolytic enzymes of a newly isolated Hymenobacter sp. CKS3 strain and showed, for the first time, that members of Hymenobacter genus have still unexplored hydrolytic potential. Crude hydrolytic enzymes, produced by the strain CKS3 on a waste medium, were incorporated into a process of bioethanol production using waste bread. The conditions for bread hydrolysis were optimized using statistical design. Waste bread hydrolysate obtained under optimal conditions (100.73 h of hydrolysis, waste bread concentration 20.36% and agitation speed 200 rpm) contained 19.89 g/l of reducing sugars. A high performance liquid chromatography of hydrolyzed waste bread samples showed that the main components of the hydrolysate were dextrins, maltotriose, maltose and glucose. When using this substrate and waste baker's yeast for ethanol production under non-optimized conditions 1.73% of ethanol was produced. The results of this study showed that a newly isolated Hymenobacter sp. CKS3 can be utilized for enzymatic hydrolysis and bioethanol production in a process relying on waste materials. Furthermore, it was demonstrated that members of Hymenobacter genus have a significant and currently unexplored potential for bio-based industrial applications.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS//
dc.rightsrestrictedAccess
dc.sourceRenewable Energy
dc.subjectHymenobacteren
dc.subjectHydrolysisen
dc.subjectWaste breaden
dc.subjectBioethanolen
dc.subjectStatistical optimizationen
dc.titleEnzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol productionen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage633
dc.citation.other152: 627-633
dc.citation.rankM21~
dc.citation.spage627
dc.citation.volume152
dc.identifier.doi10.1016/j.renene.2020.01.101
dc.identifier.rcubconv_6130
dc.identifier.scopus2-s2.0-85078430225
dc.identifier.wos000536949600052
dc.type.versionpublishedVersion


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