Synthesis and characterization of hydrogels based on 2-hydroxyethyl acrylate and itaconic acid for controlled release of therapeutically active metal ions аpplication.

Abstract

In this doctoral dissertation, the novel polymeric materials were synthesized as carriers for the incorporation and controlled release of therapeutically active metal ions, for the application in wound treatment and soft tissue regeneration. Ability to control the process of local release of incorporated active agent and managеment/prevention of infections, along with the appropriate biological activity, provides successful wound healing, which is the goal of this work. pH and temperature sensitive hydrogels based on 2-hydroxyethyl acrylate and itaconic acid were synthesized by free radical polymerization, whereby the itaconic acid content was varied which resulted in H hydrogel series. CI hydrogel series was obtained by incorporation of copper(II) ions in synthesized hydrogels. In order to evaluate the influence of the incorporated metal form on the characteristics and behavior of the hydrogels, the chemical reduction of copper(II) ions was conducted and CR hydrogel series was obtained. The prepared hydrogel series were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), differential scanning calorimetry (DSC) and monitoring of swelling kinetics, as well as pH and temperature swelling behaviour...

U ovoj doktorskoj disertaciji sintetisani su novi polimerni materijali kao nosači za ugradnju i kontrolisano otpuštanje terapeutski aktivnih jona metala, za primenu u tretmanu rana i regeneraciji mekog tkiva. Sposobnost kontrole procesa lokalnog otpuštanja ugrađenog aktivnog agensa, kao i kontrole postojećih i sprečavanje nastanka novih infekcija, uz odgovarajuću biološku aktivnost, obezbeđuje uspešno zarastanje rana, što predstavlja cilj ovog rada. Polimerizacijom preko slobodnih radikala sintetisani su rN i temperaturno osetljivi hidrogelovi na bazi 2-hidroksietil akrilata i itakonske kiseline, pri čemu je variran sadržaj itakonske kiseline i na taj način dobijena N serija hidrogelova. Ugradnjom bakar(II) jona u sintetisane hidrogelove dobijeni su hidrogelovi CI serije. U cilju ispitivanja uticaja oblika ugrađenog metala na svojstva i ponašanje hidrogelova, izvršena je hemijska redukcija bakar(II) jona i tako dobijena CR serija hidrogelova sa redukovanim bakrom. Karakterizacija sve tri serije hidrogelova izvršena je primenom infracrvene spektroskopije Furijeovom (Fourier-ovom) transformacijom (FTIR), skenirajuće elektronske mikroskopije (SEM), dinamičko-mehaničke analize (DMA), diferencijalno skenirajuće kalorimetrije (DSK) i praćenjem kinetike bubrenja, kao i rN i temperaturno osetljivog ponašanja pri bubrenju...