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dc.creatorDragoj, Miodrag
dc.creatorStojkovska, Jasmina
dc.creatorStankovic, Tijana
dc.creatorDinic, Jelena
dc.creatorPodolski-Renic, Ana
dc.creatorObradović, Bojana
dc.creatorPesic, Milica
dc.date.accessioned2022-03-04T11:15:53Z
dc.date.available2022-03-04T11:15:53Z
dc.date.issued2021
dc.identifier.issn2076-3425
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/4783
dc.description.abstractBackground: Various three-dimensional (3D) glioblastoma cell culture models have a limited duration of viability. Our aim was to develop a long-term 3D glioblastoma model, which is necessary for reliable drug response studies. Methods: Human U87 glioblastoma cells were cultured in alginate microfibers for 28 days. Cell growth, viability, morphology, and aggregation in 3D culture were monitored by fluorescent and confocal microscopy upon calcein-AM/propidium iodide (CAM/PI) staining every seven days. The glioblastoma 3D model was validated using temozolomide (TMZ) treatments 3 days in a row with a recovery period. Cell viability by MTT and resistance-related gene expression (MGMT and ABCB1) by qPCR were assessed after 28 days. The same TMZ treatment schedule was applied in 2D U87 cell culture for comparison purposes. Results: Within a long-term 3D model system in alginate fibers, U87 cells remained viable for up to 28 days. On day 7, cells formed visible aggregates oriented to the microfiber periphery. TMZ treatment reduced cell growth but increased drug resistance-related gene expression. The latter effect was more pronounced in 3D compared to 2D cell culture. Conclusion: Herein, we described a long-term glioblastoma 3D model system that could be particularly helpful for drug testing and treatment optimization.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceBrain Sciences
dc.subjectglioblastomaen
dc.subject3D cell cultureen
dc.subjectalginate hydrogelen
dc.subjecttemozolomideen
dc.subjectdrug resistanceen
dc.titleDevelopment and Validation of a Long-Term 3D Glioblastoma Cell Culture in Alginate Microfibers as a Novel Bio-Mimicking Model System for Preclinical Drug Testingen
dc.typearticle
dc.rights.licenseBY
dc.citation.issue8
dc.citation.other11(8): -
dc.citation.rankM22
dc.citation.volume11
dc.identifier.doi10.3390/brainsci11081025
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/7320/4779.pdf
dc.identifier.pmid34439644
dc.identifier.scopus2-s2.0-85112073527
dc.identifier.wos000688937200001
dc.type.versionpublishedVersion


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