Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology
Authors
Miljković, MionaDavidović, Slađana
Đukić-Vuković, Aleksandra
Ilic, Mila, V
Simović, Milica
Rajilić-Stojanović, Mirjana
Dimitrijević-Branković, Suzana
Article (Published version)
Metadata
Show full item recordAbstract
Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with su...gar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.
Keywords:
headspace / gas chromatography / alcoholic beverages / cosmeticsSource:
Hemijska industrija, 2021, 75, 3, 135-146Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
DOI: 10.2298/HEMIND200710015M
ISSN: 0367-598X
WoS: 000698039600001
Scopus: 2-s2.0-85111978209
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Miljković, Miona AU - Davidović, Slađana AU - Đukić-Vuković, Aleksandra AU - Ilic, Mila, V AU - Simović, Milica AU - Rajilić-Stojanović, Mirjana AU - Dimitrijević-Branković, Suzana PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4791 AB - Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy. T2 - Hemijska industrija T1 - Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology EP - 146 IS - 3 SP - 135 VL - 75 DO - 10.2298/HEMIND200710015M ER -
@article{ author = "Miljković, Miona and Davidović, Slađana and Đukić-Vuković, Aleksandra and Ilic, Mila, V and Simović, Milica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana", year = "2021", abstract = "Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.", journal = "Hemijska industrija", title = "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology", pages = "146-135", number = "3", volume = "75", doi = "10.2298/HEMIND200710015M" }
Miljković, M., Davidović, S., Đukić-Vuković, A., Ilic, M. V., Simović, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija, 75(3), 135-146. https://doi.org/10.2298/HEMIND200710015M
Miljković M, Davidović S, Đukić-Vuković A, Ilic MV, Simović M, Rajilić-Stojanović M, Dimitrijević-Branković S. Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija. 2021;75(3):135-146. doi:10.2298/HEMIND200710015M .
Miljković, Miona, Davidović, Slađana, Đukić-Vuković, Aleksandra, Ilic, Mila, V, Simović, Milica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology" in Hemijska industrija, 75, no. 3 (2021):135-146, https://doi.org/10.2298/HEMIND200710015M . .