The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme
Samo za registrovane korisnike
2021
Autori
Jovanović, AleksandraĐorđević, Verica
Petrović, Predrag
Pljevljakusic, Dejan S.
Zdunic, Gordana M.
Savikin, Katarina P.
Bugarski, Branko
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 +/- 0.1 mg gallic acid equivalents (GAE)/L and 16.8 +/- 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucumnide and salvianolic acid I (185.2 +/- 7.3, 141.6 +/- 3.4 and 76.4 +/- 2.1 mu g/mL, respectively). The best antioxidant recovery was achieved with particles of... 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica.
Ključne reči:
Antimicrobial activity / Antioxidant activity / Flavonoids / HPLC / PolyphenolsIzvor:
Journal of Applied Research on Medicinal and Aromatic Plants, 2021, 25Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200003 (Institut za proučavanje lekovitog bilja 'Dr Josif Pančić', Beograd) (RS-MESTD-inst-2020-200003)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-MESTD-inst-2020-200287)
DOI: 10.1016/j.jarmap.2021.100328
ISSN: 2214-7861
WoS: 000697676700016
Scopus: 2-s2.0-85108383789
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Jovanović, Aleksandra AU - Đorđević, Verica AU - Petrović, Predrag AU - Pljevljakusic, Dejan S. AU - Zdunic, Gordana M. AU - Savikin, Katarina P. AU - Bugarski, Branko PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4794 AB - The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 +/- 0.1 mg gallic acid equivalents (GAE)/L and 16.8 +/- 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucumnide and salvianolic acid I (185.2 +/- 7.3, 141.6 +/- 3.4 and 76.4 +/- 2.1 mu g/mL, respectively). The best antioxidant recovery was achieved with particles of 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica. T2 - Journal of Applied Research on Medicinal and Aromatic Plants T1 - The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme VL - 25 DO - 10.1016/j.jarmap.2021.100328 ER -
@article{ author = "Jovanović, Aleksandra and Đorđević, Verica and Petrović, Predrag and Pljevljakusic, Dejan S. and Zdunic, Gordana M. and Savikin, Katarina P. and Bugarski, Branko", year = "2021", abstract = "The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 +/- 0.1 mg gallic acid equivalents (GAE)/L and 16.8 +/- 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucumnide and salvianolic acid I (185.2 +/- 7.3, 141.6 +/- 3.4 and 76.4 +/- 2.1 mu g/mL, respectively). The best antioxidant recovery was achieved with particles of 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica.", journal = "Journal of Applied Research on Medicinal and Aromatic Plants", title = "The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme", volume = "25", doi = "10.1016/j.jarmap.2021.100328" }
Jovanović, A., Đorđević, V., Petrović, P., Pljevljakusic, D. S., Zdunic, G. M., Savikin, K. P.,& Bugarski, B.. (2021). The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme. in Journal of Applied Research on Medicinal and Aromatic Plants, 25. https://doi.org/10.1016/j.jarmap.2021.100328
Jovanović A, Đorđević V, Petrović P, Pljevljakusic DS, Zdunic GM, Savikin KP, Bugarski B. The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme. in Journal of Applied Research on Medicinal and Aromatic Plants. 2021;25. doi:10.1016/j.jarmap.2021.100328 .
Jovanović, Aleksandra, Đorđević, Verica, Petrović, Predrag, Pljevljakusic, Dejan S., Zdunic, Gordana M., Savikin, Katarina P., Bugarski, Branko, "The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme" in Journal of Applied Research on Medicinal and Aromatic Plants, 25 (2021), https://doi.org/10.1016/j.jarmap.2021.100328 . .